Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Thomas Gundinger"'
Publikováno v:
Fermentation, Vol 8, Iss 4, p 181 (2022)
Auto-inducible promoter systems have been reported to increase soluble product formation in the periplasm of E. coli compared to inducer-dependent systems. In this study, we investigated the phosphate (PO4)-sensitive phoA expression system (pAT) for
Externí odkaz:
https://doaj.org/article/375faaa2ed994ec8b098374fada0458b
Publikováno v:
Pharmaceutics, Vol 12, Iss 4, p 382 (2020)
Protein freeze-thawing is frequently used to stabilize and store recombinantly produced proteins after different unit operations in upstream and downstream processing. However, freeze-thawing is often accompanied by product damage and, hence, loss of
Externí odkaz:
https://doaj.org/article/52bb2cb79e1244fa9184a2bb7259c2bf
Autor:
Barbara Danneels, Magali Tanghe, Henk-Jan Joosten, Thomas Gundinger, Oliver Spadiut, Ingeborg Stals, Tom Desmet
Publikováno v:
PLoS ONE, Vol 12, Iss 5, p e0178446 (2017)
Lytic polysaccharide monooxygenases (LPMOs) have changed our understanding of lignocellulosic degradation dramatically over the last years. These metalloproteins catalyze oxidative cleavage of recalcitrant polysaccharides and can act on the C1 and/or
Externí odkaz:
https://doaj.org/article/9d405abd7ef249a29644c1e4ea1bfb51
Publikováno v:
Journal of Chromatography B. 1083:242-248
Antibody fragments (Fabs) represent a highly interesting class of biopharmaceuticals with a broad range of medical applications. They bind antigens comparable to full length monoclonal antibodies, but are smaller in size allowing increased tissue pen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::272b3640833bd0f95e2c82a22461f2b5
https://doi.org/10.1016/j.jchromb.2018.02.044
https://doi.org/10.1016/j.jchromb.2018.02.044
Autor:
Oliver Spadiut, Thomas Gundinger
Publikováno v:
Protein expression and purification. 165
Primary recovery of recombinant proteins from E. coli often describes a major challenge in downstream processing. After product release, the target protein usually accounts for only a small amount of total protein and has to be separated from a compl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::866620928d64916488b8c9ec9096047e
https://pubmed.ncbi.nlm.nih.gov/31560987
https://pubmed.ncbi.nlm.nih.gov/31560987
Autor:
Thomas, Gundinger, Oliver, Spadiut
Publikováno v:
Journal of biotechnology
Horseradish peroxidase (HRP) is used in various biotechnological and medical applications. Since its isolation from plant provides several disadvantages, the bacterium Escherichia coli was tested as recombinant expression host in former studies. Howe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e5872309154b9c9fdb938995b68e625f
https://pubmed.ncbi.nlm.nih.gov/28288816
https://pubmed.ncbi.nlm.nih.gov/28288816
Autor:
Oliver Spadiut, Tom Desmet, Henk-Jan Joosten, Magali Tanghe, Barbara Danneels, Thomas Gundinger, Ingeborg Stals
Publikováno v:
PLOS ONE
PLoS ONE
PLoS ONE, Vol 12, Iss 5, p e0178446 (2017)
PLoS ONE
PLoS ONE, Vol 12, Iss 5, p e0178446 (2017)
Lytic polysaccharide monooxygenases ( LPMOs) have changed our understanding of lignocellulosic degradation dramatically over the last years. These metalloproteins catalyze oxidative cleavage of recalcitrant polysaccharides and can act on the C1 and/o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::602b485809e38d7939696f8887f03e89
https://doi.org/10.1371/journal.pone.0178446
https://doi.org/10.1371/journal.pone.0178446