Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Thomas G. Spring"'
Autor:
Tracey L. Colpitts, Stephen D. Stroupe, J. J. Russell, Shelley R. Holets-McCormack, Thomas G. Spring
Publikováno v:
Clinical Chemistry. 50:1921-1929
Background: Conventional methods for conjugation of macromolecules, such as antibodies and reporter groups, typically yield a mixture ranging from unconjugated starting materials to large aggregates. We explored the use of a solid-phase process to al
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2310efe9e18d3dcc6e7ad63a661ea43
https://doi.org/10.1373/clinchem.2004.036681
https://doi.org/10.1373/clinchem.2004.036681
Autor:
Stephen D. Stroupe, John C. Russell, Tracey L. Colpitts, and Andrew D. Vogt, Sie-Ting Wong, Thomas G. Spring, Shelley R. Holets-McCormack
Publikováno v:
Bioconjugate Chemistry. 13:958-965
We have developed a solid-phase procedure for protein-protein conjugation that gives greater control over product size and composition than previous methods. Conjugates are assembled by sequential addition of activated proteins to the support under c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d368653b989c70e68040179bbadce24f
https://doi.org/10.1021/bc0255438
https://doi.org/10.1021/bc0255438
Autor:
David M. Finley, Thomas G. Spring, Helen Xie, Kevin R. Rupprecht, Sergey Y. Tetin, Beth C. Trudeau, Joan E. Peart, Sylvia C. Saldana, Elaine Brate, Shelley R. Holets-McCormack, Carol Ramsay, Jonathan Grote, Phil Wang, Pan F. Ozaeta, David Pacenti, Ryan E. Piktel
Publikováno v:
Clinical biochemistry. 43(13-14)
Objective : We investigated the mechanism by which the ARCHITECT cyclosporine (CsA) chemiluminescent microparticle immunoassay (CMIA) eliminates cross-reactivity to CsA metabolites AM1 and AM9, despite its use of a monoclonal antibody which shows cro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bcf234d062071b3a3524ede36d18370
https://pubmed.ncbi.nlm.nih.gov/20599875
https://pubmed.ncbi.nlm.nih.gov/20599875
Autor:
Sai-Hung Yu, Thomas G. Spring
Publikováno v:
Analytical Biochemistry. 85:287-290
A convenient method to store polyacrylamide gel slabs is described. The method involves sealing the wet gels between transparent plastic sheets using a kitchen-type bag sealer. The sealed gels are suitable for long-term storage, photography, and dens
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fae3de970fe0f8b02a4290a5a0b6616c
https://doi.org/10.1016/0003-2697(78)90302-0
https://doi.org/10.1016/0003-2697(78)90302-0
Autor:
Sai-Hung Yu, Thomas G. Spring
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 492:20-28
Chromatographically isolated subfractions of calf thymus H1 histone have been covalently coupled to agarose beads and tested for their ability to form complexes with the non-histone proteins HMG1 and HMG2 (High Mobility Group proteins, Walker, J.M.,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4abae8298979cbc76401752cc03b99d9
https://doi.org/10.1016/0005-2795(77)90210-0
https://doi.org/10.1016/0005-2795(77)90210-0
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 563:313-319
A simple and rapid method is described for the purification of supercoiled PM2 DNA by affinity chromatography on columns of H1 histone covalently coupled to agarose. The method does not require the use of intercalating agents or ultracentrifugation p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4251455317ea3f6838d4cd8b88b2516f
https://doi.org/10.1016/0005-2787(79)90050-9
https://doi.org/10.1016/0005-2787(79)90050-9
Autor:
Thomas G. Spring, Finn Wold
Publikováno v:
Biochemistry. 10:4655-4660
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::43ea2748e1813fd0b151f4745a7d2f79
https://doi.org/10.1021/bi00801a010
https://doi.org/10.1021/bi00801a010
Autor:
Finn Wold, Thomas G. Spring
Publikováno v:
Biochemistry. 10:4649-4654
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1f29094e4fb820946885a0ca73440d7e
https://doi.org/10.1021/bi00801a009
https://doi.org/10.1021/bi00801a009
Autor:
Thomas G. Spring, Finn Wold
Publikováno v:
Journal of Biological Chemistry. 246:6797-6802
Enolase has been purified from aqueous extracts of Escherichia coli acetone powder by (a) heat treatment, (b) fractionation with acetone, (c) TEAE-cellulose chromatography, (d) Sephadex G-100 chromatography, and (e) crystallization. The purified, cry
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::775a1f4a30cfecc8097b0cc1869facd8
https://doi.org/10.1016/s0021-9258(19)45916-4
https://doi.org/10.1016/s0021-9258(19)45916-4
Autor:
Thomas G. Spring, R. David Cole
Publisher Summary This chapter describes the techniques for chromatographic fractionation of histones. There are two chromatographic methods applicable to histones: gel filtration and ion exchange. Neither technique, even with more recent improvement
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07bffbcddf74023b8c4d20c0baf4b588
https://doi.org/10.1016/s0091-679x(08)60102-8
https://doi.org/10.1016/s0091-679x(08)60102-8
