Toward Single-Molecule Optical Mapping of the Epigenome

Autor: Ron R. Lin, Robin Samuel, Michal Levy-Sakin, Anna Gottfried, Elmar Weinhold, Assaf Grunwald, Thomas Dertinger, Sam On Ho, Soohong Kim, Xavier Michalet, Shimon Weiss, Younggyu Kim, Yuval Ebenstein, Natalie R. Gassman, Andrew S. Kim, Ryan A. Colyer, Josh Antelman, SangYoon Chung

Publikováno v: Levy-Sakin, Michal; Grunwald, Assaf; Kim, Soohong; Gassman, Natalie R; Gottfried, Anna; Antelman, Josh; et al.(2014). Toward single-molecule optical mapping of the epigenome.. ACS nano, 8(1), 14-26. doi: 10.1021/nn4050694. UCLA: Retrieved from: http://www.escholarship.org/uc/item/2467z7cj
ACS nano, vol 8, iss 1

The past decade has seen an explosive growth in the utilization of single-molecule techniques for the study of complex systems. The ability to resolve phenomena otherwise masked by ensemble averaging has made these approaches especially attractive fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd4a12753f5134882032b38815a3407c
https://doi.org/10.1021/nn4050694

Widely accessible method for superresolution fluorescence imaging of living systems

Autor: Jin Zhang, Peter Dedecker, Gary C. H. Mo, Thomas Dertinger

Publikováno v: Proceedings of the National Academy of Sciences. 109:10909-10914

Superresolution fluorescence microscopy overcomes the diffraction resolution barrier and allows the molecular intricacies of life to be revealed with greatly enhanced detail. However, many current superresolution techniques still face limitations and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc7e29b8ce048e55a84d31f16232f085
https://doi.org/10.1073/pnas.1204917109

Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)

Autor: Thomas Dertinger, Jörg Enderlein, Ryan A. Colyer, Gopal Iyer, Shimon Weiss

Publikováno v: Proceedings of the National Academy of Sciences. 106:22287-22292

Super-resolution optical microscopy is a rapidly evolving area of fluorescence microscopy with a tremendous potential for impacting many fields of science. Several super-resolution methods have been developed over the last decade, all capable of over

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e29ab47f4bb42e5d815cd492d5f88059
https://doi.org/10.1073/pnas.0907866106

Comparison of optical saturation effects in conventional and dual-focus fluorescence correlation spectroscopy

Autor: Thomas Dertinger, Felix Koberling, Jörg Enderlein, Anastasia Loman

Publikováno v: Chemical Physics Letters. 459:18-21

We present diffusion measurements of the fluorescent dye Cy5 in aqueous solutions using conventional fluorescence correlation spectroscopy (FCS) and the recently introduced dual-focus fluorescence correlation spectroscopy (2fFCS). We study the sensit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f07cd477971de1ce9f1d420b688d9673
https://doi.org/10.1016/j.cplett.2008.05.018

Performance of Fluorescence Correlation Spectroscopy for Measuring Diffusion and Concentration

Autor: Thomas Dertinger, Ingo Gregor, Digambara Patra, U. Benjamin Kaupp, Jörg Enderlein

Publikováno v: ChemPhysChem. 6:2324-2336

Fluorescence correlation spectroscopy (FCS) has become an important tool for measuring diffusion, concentration, and molecular interactions of cellular components. The interpretation of FCS data critically depends on the measurement set-up. Here, we

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd5a61fc944f98ccf5cf43cad681328a
https://doi.org/10.1002/cphc.200500414