Flagella disruption in Bacillus subtilis increases amylase production yield

Autor: Annaleigh Ohrt Fehler, Thomas Beuchert Kallehauge, Adrian Sven Geissler, Enrique González-Tortuero, Stefan Ernst Seemann, Jan Gorodkin, Jeppe Vinther

Publikováno v: Microbial Cell Factories, Vol 21, Iss 1, Pp 1-11 (2022)

Abstract Background Bacillus subtilis is a Gram-positive bacterium used as a cell factory for protein production. Over the last decades, the continued optimization of production strains has increased yields of enzymes, such as amylases, and made comm

Externí odkaz: https://doaj.org/article/04ef93e9455741768687ee8b644328e0

BSGatlas: a unified

Autor: Adrian Sven, Geissler, Christian, Anthon, Ferhat, Alkan, Enrique, González-Tortuero, Line Dahl, Poulsen, Thomas Beuchert, Kallehauge, Anne, Breüner, Stefan Ernst, Seemann, Jeppe, Vinther, Jan, Gorodkin

Publikováno v: Microbial Genomics

A large part of our current understanding of gene regulation in Gram-positive bacteria is based on Bacillus subtilis , as it is one of the most well studied bacterial model systems. The rapid growth in data concerning its molecular and genomic biolog

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::98caddc191271fc7b28d6c2e53c4ea53
https://pubmed.ncbi.nlm.nih.gov/33539279

The BSGatlas: An enhanced annotation of genes and transcripts for the Bacillus subtilis genome with improved information access

Autor: Jan Gorodkin, Enrique González-Tortuero, Jeppe Vinther, Thomas Beuchert Kallehauge, Stefan E. Seemann, Line Dahl Poulsen, Christian Anthon, Adrian Sven Geissler

The genome of Bacillus subtilis continues to provide exiting genomic insights. However, the growing collective genomic knowledge about this micro-organism is spread across multiple annotation resources. Thus, the full annotation is not directly acces

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e05f434a331eae921a3130834088e28d

Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion

Autor: Shangzhong Li, Lise Marie Grav, Bernhard O. Palsson, Michael J. Betenbaugh, Amir Feizi, Deniz Baycin Hizal, Gyun Min Lee, Nathan E. Lewis, Daniel Ley, Jens Nielsen, Bjørn G. Voldborg, Jahir M. Gutierrez, Helene Faustrup Kildegaard, Thomas Beuchert Kallehauge, Hooman Hefzi

Publikováno v: Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature communications, vol 11, iss 1
Gutierrez, J M, Feizi, A, Li, S, Kallehauge, T B, Hefzi, H, Grav, L M, Ley, D, Baycin Hizal, D, Betenbaugh, M J, Voldborg, B G, Kildegaard, H F, Min Lee, G, Palsson, B O, Nielsen, J & Lewis, N E 2020, ' Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion ', Nature Communications, vol. 11, no. 1, 68 . https://doi.org/10.1038/s41467-019-13867-y

In mammalian cells, >25% of synthesized proteins are exported through the secretory pathway. The pathway complexity, however, obfuscates its impact on the secretion of different proteins. Unraveling its impact on diverse proteins is particularly impo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06a1d2e239503ede991b1043373b042b
https://pubmed.ncbi.nlm.nih.gov/31896772

Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion

Autor: Thomas Beuchert Kallehauge, Tae Kwang Ha, Shangzhong Li, Mikael Rørdam Andersen, Lasse Ebdrup Pedersen, Nathan E. Lewis, Gyun Min Lee, Helene Faustrup Kildegaard, Daniel Ley

Publikováno v: Beuchert Kallehauge, T, Li, S, Pedersen, L E, Kwang Ha, T, Ley, D, Andersen, M R, Kildegaard, H F, Min Lee, G & Lewis, N E 2017, ' Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion ', Scientific Reports, vol. 7, 40388 . https://doi.org/10.1038/srep40388
Scientific reports, vol 7, iss 1
Scientific Reports
Beuchert Kallehauge, T, Li, S, Pedersen, L E, Kwang Ha, T, Ley, D, Andersen, M R, Kildegaard, H F, Min Lee, G & Lewis, N E 2017, ' Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion ' Scientific Reports, vol 7, 40388 . DOI: 10.1038/srep40388

Recombinant protein production coopts the host cell machinery to provide high protein yields of industrial enzymes or biotherapeutics. However, since protein translation is energetically expensive and tightly controlled, it is unclear if highly expre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d7fe3d53523abb3eb5858101dba355f