Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Thomas Averell Houze"'
Autor:
Thomas Averell Houze, Bengt Gustavsson
Publikováno v:
BioTechniques, Vol 21, Iss 5, Pp 774-778 (1996)
Externí odkaz:
https://doaj.org/article/e69e983d97b24ce1a55562c82052ce46
Publikováno v:
Tumor Biology. 18:53-68
We describe a new, simple and reliable semiautomated strategy for quantifying mRNA from archival specimens by using oligo(dT)25 paramagnetic beads and the reverse-transcriptase polymerase chain reaction (PCR) coupled with quantitative digital image a
Autor:
Thomas Averell Houze, Bengt Gustavsson
Publikováno v:
BioTechniques. 21:1074-1082
We describe a novel approach for the isolation and quantification of mRNA from archival specimens by using oligo(dT)25 paramagnetic beads and the reverse transcription polymerase chain reaction (RTPCR). A preliminary study is conducted to evaluate ex
Autor:
Bengt Gustavsson, Thomas Averell Houze
Publikováno v:
BioTechniques. 21(5)
Publikováno v:
Cell biology international. 20(9)
The secretion of interferon-gamma (IFN-gamma) by natural killer (NK) cells following in vitro stimulation with interleukin-2 (IL-2) is inhibited by co-incubation with autologous monocytes at a transcriptional level by more than sixty-fold. In this st
Autor:
O Strannegård, Markus Hansson, Svante Hermodsson, Kristoffer Hellstrand, Cecil Czerkinsky, Thomas Averell Houze, A. Asea
Publikováno v:
Clinical and experimental immunology. 105(2)
Monocytes, recovered from human peripheral blood by counter-current centrifugal elutriation, effectively inhibit the production of IFN-γ by CD3−/56+ NK cells in response to IL-2. This study aimed at defining the nature of the inhibitory signal, pa
Autor:
Goran Hansson, Bengt Gustavsson, Per-Anders Larsson, Thomas Averell Houze, Lars Larsson, Alexzander Asea
Publikováno v:
Scopus-Elsevier
We describe a simplified and reliable polymerase chain reaction (PCR) method to quantify thymidylate synthase (TS) gene expression levels from clinical human tumor biopsy samples as small as 100 mg using the beta-actin housekeeping gene as a referenc