Výsledky vyhledávání - "Thomas A Kunkel"

Akademický článek

High fidelity DNA ligation prevents single base insertions in the yeast genome

Autor: Jessica S. Williams, Scott. A. Lujan, Mercedes E. Arana, Adam B. Burkholder, Percy P. Tumbale, R. Scott Williams, Thomas A. Kunkel

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-12 (2024)

Abstract Finalization of eukaryotic nuclear DNA replication relies on DNA ligase 1 (LIG1) to seal DNA nicks generated during Okazaki Fragment Maturation (OFM). Using a mutational reporter in Saccharomyces cerevisiae, we previously showed that mutatio

Externí odkaz: https://doaj.org/article/b31b217e5a9e48d9b498d08517dfc52e

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Akademický článek

Increased and imbalanced dNTP pools symmetrically promote both leading and lagging strand replication infidelity.

Autor: Robert J Buckland, Danielle L Watt, Balasubramanyam Chittoor, Anna Karin Nilsson, Thomas A Kunkel, Andrei Chabes

Publikováno v: PLoS Genetics, Vol 10, Iss 12, p e1004846 (2014)

The fidelity of DNA replication requires an appropriate balance of dNTPs, yet the nascent leading and lagging strands of the nuclear genome are primarily synthesized by replicases that differ in subunit composition, protein partnerships and biochemic

Externí odkaz: https://doaj.org/article/4ccd5cff9c9f41b1864a275563c41488

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Akademický článek

Mismatch repair balances leading and lagging strand DNA replication fidelity.

Autor: Scott A Lujan, Jessica S Williams, Zachary F Pursell, Amy A Abdulovic-Cui, Alan B Clark, Stephanie A Nick McElhinny, Thomas A Kunkel

Publikováno v: PLoS Genetics, Vol 8, Iss 10, p e1003016 (2012)

The two DNA strands of the nuclear genome are replicated asymmetrically using three DNA polymerases, α, δ, and ε. Current evidence suggests that DNA polymerase ε (Pol ε) is the primary leading strand replicase, whereas Pols α and δ primarily p

Externí odkaz: https://doaj.org/article/4ffe57f571794dcaa7c510f291e09901

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Akademický článek

The major roles of DNA polymerases epsilon and delta at the eukaryotic replication fork are evolutionarily conserved.

Autor: Izumi Miyabe, Thomas A Kunkel, Antony M Carr

Publikováno v: PLoS Genetics, Vol 7, Iss 12, p e1002407 (2011)

Coordinated replication of eukaryotic genomes is intrinsically asymmetric, with continuous leading strand synthesis preceding discontinuous lagging strand synthesis. Here we provide two types of evidence indicating that, in fission yeast, these two b

Externí odkaz: https://doaj.org/article/dfb36560d8a845ea839967e4cf30f3e6

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Akademický článek

Unexpected role for Helicobacter pylori DNA polymerase I as a source of genetic variability.

Autor: María-Victoria García-Ortíz, Stéphanie Marsin, Mercedes E Arana, Didier Gasparutto, Raphaël Guérois, Thomas A Kunkel, J Pablo Radicella

Publikováno v: PLoS Genetics, Vol 7, Iss 6, p e1002152 (2011)

Helicobacter pylori, a human pathogen infecting about half of the world population, is characterised by its large intraspecies variability. Its genome plasticity has been invoked as the basis for its high adaptation capacity. Consistent with its smal

Externí odkaz: https://doaj.org/article/5ec3512f6b754b0d8935bb17ddd4e756

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Akademický článek

Mismatch repair-independent increase in spontaneous mutagenesis in yeast lacking non-essential subunits of DNA polymerase ε.

Autor: Anna Aksenova, Kirill Volkov, Jaroslaw Maceluch, Zachary F Pursell, Igor B Rogozin, Thomas A Kunkel, Youri I Pavlov, Erik Johansson

Publikováno v: PLoS Genetics, Vol 6, Iss 11, p e1001209 (2010)

Yeast DNA polymerase ε (Pol ε) is a highly accurate and processive enzyme that participates in nuclear DNA replication of the leading strand template. In addition to a large subunit (Pol2) harboring the polymerase and proofreading exonuclease activ

Externí odkaz: https://doaj.org/article/71e5bfc8f21d485e8204bf7838102d4d

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Akademický článek

Characterization of a natural mutator variant of human DNA polymerase lambda which promotes chromosomal instability by compromising NHEJ.

Autor: Gloria Terrados, Jean-Pascal Capp, Yvan Canitrot, Miguel García-Díaz, Katarzyna Bebenek, Tomas Kirchhoff, Alberto Villanueva, François Boudsocq, Valérie Bergoglio, Christophe Cazaux, Thomas A Kunkel, Jean-Sébastien Hoffmann, Luis Blanco

Publikováno v: PLoS ONE, Vol 4, Iss 10, p e7290 (2009)

BACKGROUND:DNA polymerase lambda (Pollambda) is a DNA repair polymerase, which likely plays a role in base excision repair (BER) and in non-homologous end joining (NHEJ) of DNA double-strand breaks (DSB). PRINCIPAL FINDINGS:Here, we described a novel

Externí odkaz: https://doaj.org/article/b2febc014bdc4b8cb41bb39952ea8e56

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Akademický článek

Molecular basis for processing of topoisomerase 1-triggered DNA damage by Apn2/APE2

Autor: Jessica S. Williams, Jessica L. Wojtaszek, Denise C. Appel, Juno Krahn, Bret D. Wallace, Evan Walsh, Thomas A. Kunkel, R. Scott Williams

Publikováno v: Cell Reports, Vol 41, Iss 1, Pp 111448- (2022)

Summary: Topoisomerase 1 (Top1) incises DNA containing ribonucleotides to generate complex DNA lesions that are resolved by APE2 (Apn2 in yeast). How Apn2 engages and processes this DNA damage is unclear. Here, we report X-ray crystal structures and

Externí odkaz: https://doaj.org/article/167a08d4af08424fa4b6a4e6dfa5bd64

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