Zobrazeno 1 - 10
of 66
pro vyhledávání: '"Thomas A Kirkland"'
Autor:
Ce Shi, Michael P Killoran, Mary P Hall, Paul Otto, Monika G Wood, Ethan Strauss, Lance P Encell, Thomas Machleidt, Keith V Wood, Thomas A Kirkland
Publikováno v:
PLoS ONE, Vol 15, Iss 12, p e0243747 (2020)
Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings. Solutions of detection reagent, containing a mixture of a substrate and luciferase enzyme that
Externí odkaz:
https://doaj.org/article/4549be00272c490db5ba97c1d7c02863
Autor:
Bryan Labra, Kshitij Parag-Sharma, John J. Powers, Sonal Srivastava, Joel R. Walker, Thomas A. Kirkland, Caroline K. Brennan, Jennifer A. Prescher, Antonio L. Amelio
Publikováno v:
iScience, Vol 27, Iss 9, Pp 110655- (2024)
Summary: The ability to visualize and track multiple biological processes in vivo in real time is highly desirable. Bioluminescence imaging (BLI) has emerged as an attractive modality for non-invasive cell tracking, with various luciferase reporters
Externí odkaz:
https://doaj.org/article/8200a627020a4d0fa1f45a1065516ab8
Autor:
Mariano Carossino, Devin Kenney, Aoife K. O’Connell, Paige Montanaro, Anna E. Tseng, Hans P. Gertje, Kyle A. Grosz, Maria Ericsson, Bertrand R. Huber, Susanna A. Kurnick, Saravanan Subramaniam, Thomas A. Kirkland, Joel R. Walker, Kevin P. Francis, Alexander D. Klose, Neal Paragas, Markus Bosmann, Mohsan Saeed, Udeni B. R. Balasuriya, Florian Douam, Nicholas A. Crossland
Publikováno v:
Viruses, Vol 14, Iss 3, p 535 (2022)
Animal models recapitulating COVID-19 are critical to enhance our understanding of SARS-CoV-2 pathogenesis. Intranasally inoculated transgenic mice expressing human angiotensin-converting enzyme 2 under the cytokeratin 18 promoter (K18-hACE2) represe
Externí odkaz:
https://doaj.org/article/f6b43f0802ad4855911f2d783877a9ee
Autor:
Yichi Su, Joel R. Walker, Mary P. Hall, Mark A. Klein, Xiang Wu, Lance P. Encell, Kerriann M. Casey, Lan Xiang Liu, Guosong Hong, Michael Z. Lin, Thomas A. Kirkland
Publikováno v:
Nature Chemical Biology.
Bioluminescence imaging (BLI) allows non-invasive visualization of cells and biochemical events in vivo and thus has become an indispensable technique in biomedical research. However, BLI in the central nervous system remains challenging because luci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2a4b82cbe780fecad5eb6fd27e347880
https://doi.org/10.1038/s41589-023-01265-x
https://doi.org/10.1038/s41589-023-01265-x
Autor:
Yan Wu, Joel R. Walker, Michael Westberg, Lin Ning, Michelle Monje, Thomas A. Kirkland, Michael Z. Lin, Yichi Su
Aberrant kinase activity contributes to the pathogenesis of brain cancers, neurodegeneration, and neuropsychiatric diseases, but identifying kinase inhibitors that function in the brain is challenging. Drug levels in blood do not predict efficacy in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dd566f99bf85c4eb7a188fdb202ff45
https://doi.org/10.1101/2022.12.30.522361
https://doi.org/10.1101/2022.12.30.522361
Autor:
Stuart K Forsyth, Emily Jost, Monika G. Wood, Valerie T Ressler, Dan Lazar, Kris Zimmermann, Connor Fitzgerald, Thomas A. Kirkland, Mary P. Hall, Thomas Machleidt, Thomas P. Smith, Kincaid Virginia, Keith V. Wood, Robin Hurst, Lance P. Encell, Melanie Dart
Publikováno v:
Analytical Chemistry. 93:5177-5184
Enzyme-linked immunosorbent assays (ELISAs) are used extensively for the detection and quantification of biomolecules in clinical diagnostics as well as in basic research. Although broadly used, the inherent complexities of ELISAs preclude their util
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f25f1b91446dc05c2258e15579e5bb58
https://doi.org/10.1021/acs.analchem.0c05074
https://doi.org/10.1021/acs.analchem.0c05074
Autor:
Rachel Friedman Ohana, Keith V. Wood, Robin Hurst, Kristopher Zimmerman, Michael M. Rosenblatt, Sergiy Levin, Thomas Machleidt, Thomas A. Kirkland
Publikováno v:
ACS Chemical Biology. 16:404-413
Identification of physiologically relevant targets for lead compounds emerging from drug discovery screens is often the rate-limiting step toward understanding their mechanism of action and potential for undesired off-target effects. To this end, we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::829a63b86a7cd4cbc4d592003531612a
https://doi.org/10.1021/acschembio.0c00987
https://doi.org/10.1021/acschembio.0c00987
Autor:
Mary P. Hall, Mark A. Kay, Yunhee Park, Joel R. Walker, Lan Xiang Liu, Louai Labanieh, Robbie G. Majzner, Namdoo Kim, Michael Z. Lin, Lance P. Encell, Jennifer R. Cochran, Kerriann M. Casey, David C. Wang, Robin Hurst, Yichi Su, Thomas A. Kirkland, Feijie Zhang, Crystal L. Mackall, Thomas P. Smith
Publikováno v:
Nature Methods. 17:852-860
Sensitive detection of two biological events in vivo has long been a goal in bioluminescence imaging. Antares, a fusion of the luciferase NanoLuc to the orange fluorescent protein CyOFP, has emerged as a bright bioluminescent reporter with orthogonal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c6045ec5f0ff65f5b482789293d1def
https://doi.org/10.1038/s41592-020-0889-6
https://doi.org/10.1038/s41592-020-0889-6
Autor:
Michelle E. Boursier, Thomas Machleidt, Christopher T. Eggers, Keith V. Wood, Robin Hurst, Kris Zimmerman, Thomas A. Kirkland, Braeden L. Butler, Rachel Friedman Ohana, Sergiy Levin
Publikováno v:
J Biol Chem
G protein-coupled receptors (GPCRs) are prominent targets to new therapeutics for a range of diseases. Comprehensive assessments of their cellular interactions with bioactive compounds, particularly in a kinetic format, are imperative to the developm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7589f7a061f6ba269a4ec6f01033956
https://doi.org/10.1074/jbc.ra119.011952
https://doi.org/10.1074/jbc.ra119.011952
Autor:
Gloria I. Perez, David Broadbent, Ahmed A. Zarea, Benedikt Dolgikh, Matthew P. Bernard, Alicia Withrow, Amelia McGill, Victoria Toomajian, Lukose K. Thampy, Jack Harkema, Joel R. Walker, Thomas A. Kirkland, Michael H. Bachmann, Jens Schmidt, Masamitsu Kanada
Publikováno v:
Advanced genetics (Hoboken, N.J.). 3(1)
Cancer cells produce heterogeneous extracellular vesicles (EVs) as mediators of intercellular communication. This study focuses on a novel method to image EV subtypes and their biodistribution in vivo. A red-shifted bioluminescence resonance energy t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d20749ab551f5169f3961907ababcfc
https://pubmed.ncbi.nlm.nih.gov/36619349
https://pubmed.ncbi.nlm.nih.gov/36619349