Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Thilo H. Pohle"'
Autor:
Yusuf B. Johari, Joseph M. Scarrott, Thilo H. Pohle, Ping Liu, Ayda Mayer, Adam J. Brown, David C. James
Publikováno v:
Biotechnology Journal. 17:2200062
Expression of recombinant genes in HEK293 cells is frequently utilized for production of recombinant proteins and viral vectors. These systems frequently employ the cytomegalovirus (CMV) promoter to drive recombinant gene transcription. However, the
Autor:
Tuck Seng Wong, Mark J. Dickman, Stephen R.P. Jaffé, Joseph M. Scarrott, David C. James, Yusuf B. Johari, Kang Lan Tee, Théo Mozzanino, Adam J. Brown, Thilo H. Pohle, Abayomi Oluwanbe Johnson, Philip J. Jackson, Ravishankar Sargur, Sheetal Maisuria, Amina Bhayat-Cammack
We describe scalable and cost-efficient production of full length, His-tagged SARS-CoV-2 spike glycoprotein trimer by CHO cells that can be used to detect SARS-CoV-2 antibodies in patient sera at high specificity and sensitivity. Transient production
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7b19657ef5d01713ee1a5599e682e8f
https://doi.org/10.1101/2020.08.07.20169441
https://doi.org/10.1101/2020.08.07.20169441
Autor:
Mark J. Dickman, Théo Mozzanino, Abayomi Oluwanbe Johnson, Tuck Seng Wong, Thilo H. Pohle, Giulia Lambiase, Kang Lan Tee, Ravishankar Sargur, Yusuf B. Johari, Sheetal Maisuria, David C. James, Adam J. Brown, Amina Bhayat-Cammack, Philip J. Jackson, Joseph M. Scarrott, Stephen R.P. Jaffé
Publikováno v:
Biotechnology and Bioengineering
We describe scalable and cost‐efficient production of full length, His‐tagged severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) spike glycoprotein trimer by Chinese hamster ovary (CHO) cells that can be used to detect SARS‐CoV‐