Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Thilak Reddy Enugala"'
Autor:
Shruthi Sridhar, Alberto Zavarise, Tiila-Riikka Kiema, Subhadra Dalwani, Tor Eriksson, Yannick Hajee, Thilak Reddy Enugala, Rik K. Wierenga, Mikael Widersten
Publikováno v:
IUCrJ, Vol 10, Iss 4, Pp 437-447 (2023)
The Fe2+-dependent E. coli enzyme FucO catalyzes the reversible interconversion of short-chain (S)-lactaldehyde and (S)-1,2-propanediol, using NADH and NAD+ as cofactors, respectively. Laboratory-directed evolution experiments have been carried out p
Externí odkaz:
https://doaj.org/article/02f505bc50b84530816ebbb6260ee660
Eight related alcohol dehydrogenases that had been originally isolated by laboratory evolution of ADH-A from Rhodococcus ruber DSM44541 for modified substrate scopes, were together with their parent wild-type, subjected to biochemical characterizatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b9d87bd7de27e33e887a146a3e9ace0
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-416371
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-416371
Autor:
Thilak Reddy Enugala, Mikael Widersten, Derar Al-Smadi, Sarah Engel, Candice Gautier, Huan Ma
Publikováno v:
Biochemistry. 57:5877-5885
d-Fructose 6-phosphate aldolase (FSA) catalyzes the asymmetric cross-aldol addition of phenylacetaldehyde and hydroxyacetone. We conducted structure-guided saturation mutagenesis of noncatalytic active-site residues to produce new FSA variants, with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0e902ea6496b33a0bcbbbf27cc6af50
https://doi.org/10.1021/acs.biochem.8b00814
https://doi.org/10.1021/acs.biochem.8b00814
Publikováno v:
Synlett. 29:1187-1190
Methods for the synthesis of phenylacetaldehydes (oxidation, one-carbon chain extension) were compared by using the synthesis of 4-methoxyphenylacetaldehyde as a model example. Oxidations of 4-methoxyphenylethanol with activated DMSO (Swern oxidation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::516550a3da47fbb47c5ae33bacef9c0b
https://doi.org/10.1055/s-0036-1591963
https://doi.org/10.1055/s-0036-1591963
Autor:
Dirk Maurer, Thao Chu, Thilak Reddy Enugala, Mikael Widersten, Emil Hamnevik, Robin Löfgren, Doreen Dobritzsch
Publikováno v:
Biochemistry. 57(7)
Laboratory evolution of alcohol dehydrogenase produced enzyme variants with improved turnover numbers with a vicinal 1,2-diol and its corresponding hydroxyketone. Crystal structure and transient kinetics analysis aids in rationalizing the new functio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bf3117f7637377e712826c9e6cb6a53
https://pubmed.ncbi.nlm.nih.gov/29384657
https://pubmed.ncbi.nlm.nih.gov/29384657
Publikováno v:
ChemBioChem. 16:2595-2598
Aldolases are potentially important biocatalysts for asymmetric synthesis of polyhydroxylated compounds. Fructose 6-phosphate aldolase (FSA) is of particular interest by virtue of its unusually relaxed dependency on phosphorylated substrates. FSA has
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ccb34b1a9d6624d8af3d36fec7daaab
https://doi.org/10.1002/cbic.201500466
https://doi.org/10.1002/cbic.201500466