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Construction, heterological expression and a simple purification of the BP region of the pneumococcal surface protein A fused in different orientations to the chemotaxis adaptor protein CheW from Thermotoga petrophila

Autor: Dmitry V. Grishin, Nikita G. Sidorov, Olga K. Parfenova, Roman V. Kurkin, Ekaterina Y. Kasap

Publikováno v: Electronic Journal of Biotechnology, Vol 71, Iss , Pp 19-27 (2024)

Background: The important challenge to the biotechnology is to find new effective fusion partners that enable to improve solubility, expression, and optimize the subsequent fine purification of the target protein. Results: The most invariant part of

Externí odkaz: https://doaj.org/article/7f3983c60ba24c1ab65f9f6e49715cb6

High-resolution structure of a modular hyperthermostable endo-β-1,4-mannanase from Thermotoga petrophila: The ancillary immunoglobulin-like module is a thermostabilizing domain

Autor: da Silva, Viviam M., Cabral, Aline D., Sperança, Marcia A., Squina, Fabio M., Muniz, João Renato C., Martin, Lydie, Nicolet, Yvain, Garcia, Wanius

Publikováno v: In BBA - Proteins and Proteomics August 2020 1868(8)

Kinetics and thermodynamics of catalysis and thermal inactivation of a novel α-amylase (Tp-AmyS) from Thermotoga petrophila.

Autor: Hameed, Uzma¹ (AUTHOR) uzmahameed@gmail.com, Ul-Haq, Ikram¹ (AUTHOR)

Publikováno v: Biocatalysis & Biotransformation. Jun2020, Vol. 38 Issue 3, p227-233. 7p. 4 Charts, 5 Graphs.

[Construction and characterization of a recombinant mutant homolog of the CheW protein from Thermotoga petrophila RKU-1].

Autor: Grishin DV; Institute of Biomedical Chemistry, Moscow, Russia., Zhdanov DD; Institute of Biomedical Chemistry, Moscow, Russia., Gladilina JA; Institute of Biomedical Chemistry, Moscow, Russia., Pokrovsky VS; Institute of Biomedical Chemistry, Moscow, Russia., Podobed OV; Institute of Biomedical Chemistry, Moscow, Russia., Pokrovskaya MV; Institute of Biomedical Chemistry, Moscow, Russia., Aleksandrova SS; Institute of Biomedical Chemistry, Moscow, Russia., Milyushkina AL; Institute of Biomedical Chemistry, Moscow, Russia., Vigovskiy MA; Institute of Biomedical Chemistry, Moscow, Russia., Sokolov NN; Institute of Biomedical Chemistry, Moscow, Russia.

Publikováno v: Biomeditsinskaia khimiia [Biomed Khim] 2018 Jan; Vol. 64 (1), pp. 53-60.