Výsledky vyhledávání - "Thermococcus hydrothermalis"

Tyrosine 39 of GH13 α-amylase from Thermococcus hydrothermalis contributes to its thermostability

Autor: Viera Horváthová, Štefan Janeček, Andrej Godány, Katarína Majzlová, Barbora Vidová

Publikováno v: Biologia. 65:408-415

The presented work is focused on the naturally thermostable α-amylase from the archaebacterium Thermococcus hydrothermalis. From the evolutionary point of view, the archaeal α-amylases are most closely related to plant α-amylases. In a wider sense

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ec936bd4f1c24f21e09a9ff4757fa95c
https://doi.org/10.2478/s11756-010-0030-x

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α-Amylase from Thermococcus hydrothermalis: Re-cloning aimed at the improved expression and hydrolysis of corn starch

Autor: Viera Horváthová, Andrej Godány, Štefan Janeček, Ernest Šturdík

Publikováno v: Enzyme and Microbial Technology. 39:1300-1305

A gene encoding the thermostable α-amylase from hyperthermophilic archaeon Thermococcus hydrothermalis was cloned in Escherichia coli . In an effort to achieve the improved expression, four DNA primers (three reverse and one forward) were designed t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::938d566d142039db510b5b99759c7905
https://doi.org/10.1016/j.enzmictec.2006.03.016

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Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis

Autor: Richard Zona, Michael J. O’Donohue, Florent Chang-Pi-Hin, Štefan Janeček

Publikováno v: European Journal of Biochemistry. 271:2863-2872

Fifty-nine amino acid sequences belonging to family 57 (GH-57) of the glycoside hydrolases were collected using the CAZy server, Pfam database and blast tools. Owing to the sequence heterogeneity of the GH-57 members, sequence alignments were perform

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https://doi.org/10.1111/j.1432-1033.2004.04144.x

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Thermococcus gammatolerans sp. nov., a hyperthermophilic archaeon from a deep-sea hydrothermal vent that resists ionizing radiation

Autor: Stéphane L'Haridon, Erwan Corre, Patrick Forterre, Edmond Jolivet, Daniel Prieur

Publikováno v: International Journal of Systematic and Evolutionary Microbiology
International Journal of Systematic and Evolutionary Microbiology, 2003, 53 (3), pp.847-851. ⟨10.1099/ijs.0.02503-0⟩

Enrichments for anaerobic organotrophic hyperthermophiles were performed with hydrothermal chimney samples collected at the Guaymas Basin (27 degrees 01' N, 111 degrees 24' W). Positive enrichments were submitted to gamma-irradiation at a dose of 30

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af2a77f532fa0eeb5f208b9c45d7008a
https://doi.org/10.1099/ijs.0.02503-0

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[Untitled]

Autor: Michael J. O’Donohue, Florent Chang-Pi-Hin, Marta Erra-Pujada, Philippe Debeire, Francis Duchiron

Publikováno v: Biotechnology Letters. 23:1273-1277

A pullulanase type II was produced in Escherichia coli using the relevant gene from Thermococcus hydrothermalis. This protein was purified and its pullulanolytic and amylolytic activities were characterised. The optimum temperature and Ca2+ concentra

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https://doi.org/10.1023/a:1010597619811

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[Untitled]

Autor: Hubert Gantelet, Francis Duchiron

Publikováno v: Biotechnology Letters. 21:71-75

A crude preparation of thermostable pullulanase from Thermococcus hydrothermalis produced glucose and maltose syrups from starches. The use of pullulanase reduced the saccharification reaction time up to 37.5%. In the case of maltose syrup production

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7e1c57b3917fcab76ea26409c48abe6d
https://doi.org/10.1023/a:1005453303688

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Purification and properties of a thermoactive and thermostable pullulanase from Thermococcushydrothermalis , a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent

Autor: H. Gantelet, F. Duchiron

Publikováno v: Applied Microbiology and Biotechnology. 49:770-777

The extremely thermophilic archaeon Thermococcus hydrothermalis, isolated from a deep-sea hydrothermal vent in the East Pacific Rise at 21°N, produced an extracellular pullulanase. This enzyme was purified 97-fold to homogeneity from cell-free cultu

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https://doi.org/10.1007/s002530051245

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A Single Step High Temperature Hydrolysis of Wheat Starch

Autor: Francis Duchiron, Estelle Legin, Alain Copinet

Publikováno v: Starch - Stärke. 50:84-89

Hydrolysis of native starch at 90°C by the α-amylase from Bacillus licheniformis and the α-glucosidase of Thermococcus hydrothermalis, an archaeum, has been investigated. The archaeal enzyme is optimally active at 110°C and pH 5.5. At 96°C, the

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https://doi.org/10.1002/(sici)1521-379x(199803)50:2/3<84::aid-star84>3.0.co

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[Untitled]

Autor: Francis Duchiron, Alain Copinet, Estelle Legin

Publikováno v: Biotechnology Letters. 20:363-367

A cell extract of Thermococcus hydrothermalis, grown for 6 h, gave α-glucosidase activity at 14.9 U/l, degrading oligosaccharides and maltose. α-Amylase, α-glucosidase and pullulanase activities were detected at 289 U/l, 13.5 U/l and 30 U/l respec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1c6caef061258e5cb8b8e5e72a7e5451
https://doi.org/10.1023/a:1005375213196

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