Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Theresa L, Kern"'
Autor:
Ramesh K. Jha, Jeremy M. Bingen, Christopher W. Johnson, Theresa L. Kern, Payal Khanna, Daniel S. Trettel, Charlie E.M. Strauss, Gregg T. Beckham, Taraka Dale
Publikováno v:
Metabolic Engineering Communications, Vol 6, Iss , Pp 33-38 (2018)
Robust fluorescence-based biosensors are emerging as critical tools for high-throughput strain improvement in synthetic biology. Many biosensors are developed in model organisms where sophisticated synthetic biology tools are also well established. H
Externí odkaz:
https://doaj.org/article/46ccefbd10164a439fdf1445d0b98b92
Autor:
Naresh Pandey, Steffi A. Davison, Malathy Krishnamurthy, Daniel S. Trettel, Chien-Chi Lo, Shawn Starkenburg, Katherine L. Wozniak, Theresa L. Kern, Sean D. Reardon, Clifford J. Unkefer, Scott P. Hennelly, Taraka Dale
Publikováno v:
ACS synthetic biology. 11(10)
Engineered microbes can be used for producing value-added chemicals from renewable feedstocks, relieving the dependency on nonrenewable resources such as petroleum. These microbes often are composed of synthetic metabolic pathways; however, one major
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e76748d6c16d70d22106556b8dd28af
https://pubmed.ncbi.nlm.nih.gov/36130255
https://pubmed.ncbi.nlm.nih.gov/36130255
Autor:
Taraka Dale, Jeremy M. Bingen, Scott P. Hennelly, Christopher W. Johnson, Ramesh K. Jha, Niju Narayanan, Gregg T. Beckham, Naresh Pandey, Theresa L. Kern, Charlie E. M. Strauss
Publikováno v:
ACS Synthetic Biology. 8:775-786
Product inhibition is a frequent bottleneck in industrial enzymes, and testing mutations to alleviate product inhibition via traditional methods remains challenging as many variants need to be tested against multiple substrate and product concentrati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8eca4454f164ead9b1c6524a3ed89ed0
https://doi.org/10.1021/acssynbio.8b00465
https://doi.org/10.1021/acssynbio.8b00465
Autor:
Christopher W. Johnson, Payal Khanna, Jeremy M. Bingen, Taraka Dale, Gregg T. Beckham, Theresa L. Kern, Ramesh K. Jha, Daniel S. Trettel, Charlie E. M. Strauss
Publikováno v:
Metabolic Engineering Communications, Vol 6, Iss, Pp 33-38 (2018)
Robust fluorescence-based biosensors are emerging as critical tools for high-throughput strain improvement in synthetic biology. Many biosensors are developed in model organisms where sophisticated synthetic biology tools are also well established. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fc9c1f00b315b6b7449a837025f52b5
https://doi.org/10.1016/j.meteno.2018.03.001
https://doi.org/10.1016/j.meteno.2018.03.001
Autor:
Emily N. Schmidt, David T. Fox, Andrew T. Koppisch, Kellan B. Finney, Charlie E. M. Strauss, Gustavo M. Canales, Ramesh K. Jha, Theresa L. Kern, Lucas B. Harrington
Publikováno v:
ACS Synthetic Biology. 6:120-129
Thermostabilization of an enzyme with complete retention of catalytic efficiency was demonstrated on recombinant 3-dehydroshikimate dehydratase (DHSase or wtAsbF) from Bacillus thuringiensis serovar konkukian 97–27 (hereafter, B. thuringiensis 97
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fe75e630e46b8b4c3806cd3cfc18bef
https://doi.org/10.1021/acssynbio.6b00159
https://doi.org/10.1021/acssynbio.6b00159
Autor:
Robert Jedrzejczak, Charlie E. M. Strauss, Andrzej Joachimiak, Youngchang Kim, Theresa L. Kern, Christine Tesar, Ramesh K. Jha
Publikováno v:
Nucleic Acids Research
A whole-cell biosensor utilizing a transcription factor (TF) is an effective tool for sensitive and selective detection of specialty chemicals or anthropogenic molecules, but requires access to an expanded repertoire of TFs. Using homology modeling a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0f6e6b3d6685f1a697c200452714514
https://doi.org/10.1093/nar/gkw687
https://doi.org/10.1093/nar/gkw687
Autor:
Sanjana Apte, Michael Zakrewsky, Amrita Banerjee, David T. Fox, Mattie R. Jones, Andrew T. Koppisch, Rico E. Del Sesto, Theresa L. Kern, Samir Mitragotri
Publikováno v:
Advanced Healthcare Materials. 5:1282-1289
Antiseptic agents are the primary arsenal to disinfect skin and prevent pathogens spreading within the host as well as into the surroundings; however the Food and Drug Administration published a report in 2015 requiring additional validation of nearl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66d3ff25156f9d8cff76ba17254a1053
https://doi.org/10.1002/adhm.201600086
https://doi.org/10.1002/adhm.201600086
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 83:1327-1340
Structure-based rational mutagenesis for engineering protein functionality has been limited by the scarcity and difficulty of obtaining crystal structures of desired proteins. On the other hand, when high-throughput selection is possible, directed ev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cf51d5bf76bace12c66a01de72ce8885
https://doi.org/10.1002/prot.24828
https://doi.org/10.1002/prot.24828
Autor:
Ramesh K, Jha, Jeremy M, Bingen, Christopher W, Johnson, Theresa L, Kern, Payal, Khanna, Daniel S, Trettel, Charlie E M, Strauss, Gregg T, Beckham, Taraka, Dale
Publikováno v:
Metabolic Engineering Communications
Robust fluorescence-based biosensors are emerging as critical tools for high-throughput strain improvement in synthetic biology. Many biosensors are developed in model organisms where sophisticated synthetic biology tools are also well established. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cccf4814ff4ad2bf52d01c8da67c046c
https://pubmed.ncbi.nlm.nih.gov/29765865
https://pubmed.ncbi.nlm.nih.gov/29765865
Publikováno v:
Nucleic Acids Research
We created a single cell sorting system to screen for enzyme activity in Escherichia coli producing 3,4 dihydroxy benzoate (34DHB). To do so, we engineered a transcription factor regulon controlling the expression of green fluorescent protein (GFP) f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fb8ceaee0f1423aeb62a8b8ef7205c2
https://doi.org/10.1093/nar/gku444
https://doi.org/10.1093/nar/gku444