Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Theresa D. Kennedy"'
Publikováno v:
European Journal of Biochemistry. 209:971-976
A cDNA library was prepared from the bulk mRNA of mature wheat embryos and screened with mixed 32P-labeled oligonucleotide probes that encoded parts of the partial amino-acid sequence for the Zn-containing Ec protein. Each DNA insert in 11 positives
Autor:
Theresa D. Kennedy, François Bernier, William J. Hurkman, Judith Frégeau, Andrew C. Cuming, Byron G. Lane, Nicholas C. Carpita, Ella Dratewka-Kos
Publikováno v:
European Journal of Biochemistry. 209:961-969
Nascent synthesis and accumulation of germin and its mRNA mark the onset of renewed growth when wheat embryos are germinated in water. Germin is a water-soluble, pepsin-resistant protein that is not found in immature embryos, or in mature embryos bef
Autor:
C Pyne, T. Vaughan, F. Altomare, Theresa D. Kennedy, Ella Dratewka-Kos, D. Walters, J.R. Munro, F. Bernier, Byron G. Lane, R. Shafai
Publikováno v:
Scopus-Elsevier
By screening approximately 10(6) plaques in a wheat DNA library with a "full-length" germin cDNA probe, two genomic clones were detected. When digested with EcoRI, one clone yielded a 2.8-kilobase pair fragment (gf-2.8) and the other yielded a 3.8-ki
Publikováno v:
Journal of Biological Chemistry. 265:12503-12512
Germin was previously shown to contain covalently bonded and adventitious glycans. The object of the present investigation was to characterize the two types of glycan. The presence of N- but not O-glycans in germin is indicated by the biosynthesis of
Publikováno v:
Canadian Journal of Biochemistry. 60:389-397
The most prominent methionine-labeled protein made when cell-free systems are programmed with bulk mRNA from dry wheat embryos has been identified with what may be the most abundant protein in dry wheat embryos. The protein has been brought to purity
Autor:
Byron G. Lane, Theresa D. Kennedy
Publikováno v:
Canadian Journal of Biochemistry and Cell Biology. 62:321-328
Various techniques have been used to characterize the rapidly migrating electrophoretic components (RMEC) in an RNA fraction which is subject to conspicuous terminal labeling (mostly adenosine) when wheat embryos are pulse labeled with 3H-labeled nuc
Publikováno v:
Journal of Biological Chemistry. 256:5802-5809
After treatment at a microsomal nuclease concentration too low to reduce the endogenous amino acid-incorporating activity of freshly prepared reticulocyte lysate, there is little, if any, intact 26 S RNA left in the ribosomes of either wheat germ or
Autor:
Sandra D'Agostino, Theresa D. Kennedy, Jeanne Orr, Anna Jaikaran, Robert Kajioka, Byron G. Lane, Zbyszko Grzelczak
Publikováno v:
Biochemistry and Cell Biology. 64:1025-1037
(1) The electrophoretic band patterns (sodium dodecyl sulfate (SDS) – polyacrylamide) for either isotopically labeled or dye-stained proteins in a wash fraction obtained by rinsing germinated wheat embryos with any of a variety of buffers are diffe
Publikováno v:
Biochemistry and Cell Biology. 65:1001-1005
The partial amino-acid sequence of the wheat-germ Ec protein has been published previously. Computer analysis failed to show a significant relation between the amino-acid sequence of Ec and the sequences of other proteins stored in the data base of t
Publikováno v:
Canadian Journal of Biochemistry. 57:1170-1175
Commercially milled wheat germ is shown to be a convenient source material for facile recovery of mass (milligram) quantities of highly purified poly(A)-rich RNA. This poly(A)-rich RNA is efficiently translated in a nuclease-treated extract of rabbit