CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury.

Autor: Marcus Keatinge, Themistoklis M Tsarouchas, Tahimina Munir, Nicola J Porter, Juan Larraz, Davide Gianni, Hui-Hsin Tsai, Catherina G Becker, David A Lyons, Thomas Becker

Publikováno v: PLoS Genetics, Vol 17, Iss 4, p e1009515 (2021)

Zebrafish exhibit robust regeneration following spinal cord injury, promoted by macrophages that control post-injury inflammation. However, the mechanistic basis of how macrophages regulate regeneration is poorly understood. To address this gap in un

Externí odkaz: https://doaj.org/article/ebb1d8e5ba6e43a49ae28bb01edfb043

Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish

Autor: Daniel Wehner, Themistoklis M. Tsarouchas, Andria Michael, Christa Haase, Gilbert Weidinger, Michell M. Reimer, Thomas Becker, Catherina G. Becker

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-17 (2017)

Following spinal injury in zebrafish, non-neural cells establish an extracellular matrix to promote axon re-growth but how this is regulated is unclear. Here, the authors show that Wnt/β-catenin signaling in fibroblast-like cells at a lesion activat

Externí odkaz: https://doaj.org/article/c3ca8d8280e24c85b351e0dcdd875413

CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury

Autor: David A. Lyons, Themistoklis M. Tsarouchas, Catherina G. Becker, Thomas Becker, Hui-Hsin Tsai, Juan Larraz, Davide Gianni, Tahimina Munir, Marcus Keatinge, Nicola J. Porter

Publikováno v: PLoS Genetics, Vol 17, Iss 4, p e1009515 (2021)
PLoS Genetics

Zebrafish exhibit robust regeneration following spinal cord injury, promoted by macrophages that control post-injury inflammation. However, the mechanistic basis of how macrophages regulate regeneration is poorly understood. To address this gap in un

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c9f2e00cacb7fb7e9f1d67c3a37a637
https://doaj.org/article/ebb1d8e5ba6e43a49ae28bb01edfb043

A Unique Macrophage Subpopulation Signals Directly to Progenitor Cells to Promote Regenerative Neurogenesis in the Zebrafish Spinal Cord

Autor: Ana-Maria Oprişoreanu, Leonardo Cavone, Neil C. Henderson, Soe Sandi, Marcus Keatinge, Thomas Becker, Beth E. P. Henderson, Erika A. Aguzzi, Themistoklis M. Tsarouchas, Daniel Wehner, Tess McCann, Louisa K. Drake, Jathurshan Selvarajah, Elisa Pedersen, Catherina G. Becker, Ross Dobie

Publikováno v: SSRN Electronic Journal.

During ontogeny, neural stem cells in the spinal cord cease production of neurons. Spinal cord injury re-initiates neurogenesis in anamniotes (amphibians and fishes), but not in mammals. It is unclear whether regenerative neurogenesis from spinal pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::20f929dd242911c3c7656968d0fd0e13
https://doi.org/10.2139/ssrn.3712669

Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish

Autor: Gianna W. Maurer, Catherina G. Becker, Jochen Ohnmacht, Antón Barreiro-Iglesias, Daniel Wehner, Dirk Sieger, Yujie Yang, Themistoklis M. Tsarouchas, Thomas Becker

Publikováno v: Development (Cambridge, England)
Ohnmacht, J, Yang, Y, Maurer, G W, Barreiro-Iglesias, A, Tsarouchas, T M, Wehner, D, Sieger, D, Becker, C G & Becker, T 2016, ' Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish ', Development, vol. 143, no. 9, pp. 1464-1474 . https://doi.org/10.1242/dev.129155
Development, 143(9), 1464-1474. Cambridge, United Kingdom: Company of Biologists (2016).

In adult zebrafish, relatively quiescent progenitor cells show lesion-induced generation of motor neurons. Developmental motor neuron generation from the spinal motor neuron progenitor domain (pMN) sharply declines at 48 hours post-fertilisation (hpf

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::378c5fe87287b1c2cfd2fd216e1416f7
http://europepmc.org/articles/PMC4986163