Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Th.H. Rozijn"'
Publikováno v:
Virology. 63:168-175
The replication of adenovirus type 5 DNA occurs by discontinuous chain propagation via short pieces of DNA. These pieces accumulate if the infected cells are treated with hydroxyurea. They have a sedimentation coefficient of 11 S corresponding to a m
Publikováno v:
Virology. 140:197-200
The DNAs of the two fastidious adenovirus species 40 and 41 (Ad40 and Ad41) have been studied by restriction endonuclease cleavage, CsCl density gradient centrifugation, and liquid-phase hybridization. For Ad40 DNA, cleavage maps were constructed for
Structure and organization of the left-terminal DNA regions of fastidious adenovirus types 40 and 41
Publikováno v:
Gene. 58:109-126
To determine whether differences in structure and organization of the transforming regions of Ad40 and Ad41 could explain the fastidious growth of these viruses, we have sequenced these regions and analyzed the structure of the corresponding mRNAs. T
Publikováno v:
Gene. 17:263-270
The hamster cell line BHK268-C31 contains two large viral inserts which both include sequences from the left-hand end of adenovirus type 5 (Ad5) DNA. One of these viral inserts has been cloned in the λ vector Charon 4A. Electron microscopic analysis
Publikováno v:
Virology. 72:99-109
The localization and fate of parental adenovirus type 5 (Ad5) DNA in KB cells at the time viral DNA replication occurs has been investigated by electron-microscope autoradiography and biochemical methods. At 18 hr after infection the majority of the
Publikováno v:
Experimental Cell Research, 70(1), 140. Elsevier
High resolution autoradiography of isolated yeast nuclei has been used to investigate the distribution of newly formed RNA in the nucleus. The nuclei were isolated after incubation of spheroplasts with 3H-uracil for 5 min. Most grains (80%) are locat
Publikováno v:
Experimental Cell Research. 80:313-321
A fraction rich in dense crescent material, as judged by electron microscopy, has been isolated from yeast nuclei. Double-labelling experiments with 14C-uracil and 3H-Me-methionine show that nearly all of the 37S and 28S ribosomal precursor RNA is co
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 195:473-483
1. A deoxyribonucleoprotein complex was isolated from Saccharomyces cerevisiae. It is composed of 36% DNA, 4% RNA and 60% protein. About 70% of the protein is acid-extractable. The complex sediments as a single band with a s°20,w of 27 S. 2. The yea
Autor:
Th.H. Rozijn, G.J.M. Tonino
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects. 91:105-112
1. 1. A method for isolating pure cell-nuclei from the yeast Saccharomyces carlsbergensis is described. The yield of nuclei ranged from 70 to 80% as judged by the recovery of deoxyribonucleic acid. 2. 2. The morphology of the nuclei was dependent upo
Publikováno v:
Experimental Cell Research. 71:33-40
In vivo methylated precursors of ribosomal RNA in yeast have been characterized on acrylamide gels. The initial ribosomal precursor in the yeast nucleus is a 37S RNA component, which is processed to a nuclear 28S RNA. Both the 37S and the 28S RNA com