Zobrazeno 1 - 10
of 253
pro vyhledávání: '"Tetrameric protein"'
Autor:
Gabriel J. Fuente‐Gómez, Alexis C. Miranda, Creighton L. Kellum, Elizabeth E. Howell, Michael R. Duff
Publikováno v:
Protein Science. 30:477-484
R67 dihydrofolate reductase (R67 DHFR) is a plasmid-encoded enzyme that confers resistance to the antibacterial drug trimethoprim. R67 DHFR is a tetramer with a single active site that is unusual as both cofactor and substrate are recognized by symme
Publikováno v:
Drug Design, Development and Therapy. 14:1057-1081
Transthyretin (TTR) is a tetrameric protein, and its dissociation, aggregation, deposition, and misfolding are linked to several human amyloid diseases. As the main transporter for thyroxine (T4) in plasma and cerebrospinal fluid, TTR contains two T4
Publikováno v:
International journal of biological macromolecules. 191
Wild-type human transthyretin (TTR) is a tetrameric protein that transports thyroxine and retinol in the blood and brain. However, a number of mutations or aging leads to destabilization of the quaternary structure of TTR, which results in dissociati
Autor:
Valérie Nicolas, Hana Valenta, Yasmina Bousmah, Utkarsh Singh, Fabienne Merola, Marie Erard, Giulia Bertolin, Hélène Pasquier, Marc Tramier
Yellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostabi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fd86dd495bf146b4feedf3edea9a0ce
https://doi.org/10.1101/2021.04.27.441613
https://doi.org/10.1101/2021.04.27.441613
Autor:
Tarick J. El-Baba, David E. Clemmer
Publikováno v:
Int J Mass Spectrom
Variable-temperature nano-electrospray ionization coupled with ion mobility spectrometry-mass spectrometry is used to investigate the thermal denaturation of the tetrameric protein concanavalin A. As the solution temperature is increased, changes in
Publikováno v:
High Pressure Research. 39:218-224
High pressure native polyacrylamide gel electrophoresis has been designed to visualize the dissociation/association process of protein complexes. This paper reports this methodology in more quantitative way by inspecting pressure dissociation of pig
Autor:
Guilherme M. Lima, Susmita Sarkar, Mirat Sojitra, Gisele Monteiro, Alexey Atrazhev, Revathi Reddy, Matthew S. Macauley, Ratmir Derda
Publikováno v:
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Universidade de São Paulo (USP)
instacron:USP
Phage display links phenotype of displayed polypeptides with DNA sequence in phage genome and offers a universal method for discovery of proteins with novel properties. Injection of phage-displayed libraries in living organisms further provides a uni
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee171cb2a1767227674b97525ca7a42b
Publikováno v:
Acta biochimica Polonica 68(1), 29-31 (2021). doi:10.18388/abp.2020_5501
Acta biochimica Polonica 68(1), 29-31 (2021). doi:10.18388/abp.2020_5501
Protein crystallographers are well aware of the trap of crystallizing E. coli proteins instead of the macromolecule of interest if heterologous recombinant protein expressi
Protein crystallographers are well aware of the trap of crystallizing E. coli proteins instead of the macromolecule of interest if heterologous recombinant protein expressi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e2a314c3ff10b46023d892f8a157f49
Akademický článek
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Publikováno v:
Frontiers in Microbiology, Vol 11 (2020)
Frontiers in Microbiology
Frontiers in Microbiology
High level expression of recombinant proteins in bacteria often results in their aggregation into inclusion bodies. Formation of inclusion bodies poses a major bottleneck in high-throughput recovery of recombinant protein. These aggregates have amylo