Combinatorial single-cell profiling of all major chromatin types with MAbID

Autor: Silke J.A. Lochs, Robin H. van der Weide, Kim L. de Luca, Tessy Korthout, Ramada E. van Beek, Hiroshi Kimura, Jop Kind

Gene expression programs result from the collective activity of many regulatory factors. To obtain insight into the mechanisms that govern gene regulation, it is imperative to study their combined mode of action and interconnectivity. However, it has

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::48ceea3792b260d90cd336cf2c83a74f
https://doi.org/10.1101/2023.01.18.524584

Direct screening for chromatin status on DNA barcodes in yeast delineates the regulome of H3K79 methylation by Dot1

Autor: Hanneke Vlaming, Thom M Molenaar, Tibor van Welsem, Deepani W Poramba-Liyanage, Desiree E Smith, Arno Velds, Liesbeth Hoekman, Tessy Korthout, Sjoerd Hendriks, AF Maarten Altelaar, Fred van Leeuwen

Publikováno v: eLife, Vol 5 (2016)

Given the frequent misregulation of chromatin in cancer, it is important to understand the cellular mechanisms that regulate chromatin structure. However, systematic screening for epigenetic regulators is challenging and often relies on laborious ass

Externí odkaz: https://doaj.org/article/e595d7f77f3849ec8660431a9e2c8ef7

The histone methyltransferase DOT1L prevents antigen-independent differentiation and safeguards epigenetic identity of CD8(+) T cells

Autor: Teun van den Brand, Tibor van Welsem, Mir Farshid Alemdehy, Joke M. M. den Haan, Sjoerd Hendriks, Jannie Borst, Fred van Leeuwen, Muhammad Aslam, Tessy Korthout, Elzo de Wit, Heinz Jacobs, Cesare Lancini, Eliza Mari Kwesi-Maliepaard, Chelsea M. McLean, Tomasz Ahrends, Hanneke Vlaming, Dieke van Dinther

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 117(34), 20706-20716. National Academy of Sciences
Kwesi-Maliepaard, E M, Aslam, M A, Alemdehy, M F, Van Den Brand, T V, McLean, C, Vlaming, H, van Welsem, T, Korthout, T, Lancini, C, Hendriks, S, Ahrends, T, van Dinther, D, den Haan, J M M, Borst, J, de Wit, E, van Leeuwen, F & Jacobs, H 2020, ' The histone methyltransferase DOT1L prevents antigen-independent differentiation and safeguards epigenetic identity of CD8 + T cells ', Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 34, pp. 20706-20716 . https://doi.org/10.1073/pnas.1920372117
Proceedings of the National Academy of Sciences, 117(34), 20706-20716. NATL ACAD SCIENCES

Significance Cytotoxic CD8+ T cells control infectious diseases as well as cancer. Memory T cells are a subset of CD8+ T cells that have previously encountered and rapidly responded to a specific antigen. Here we identified the epigenetic writer DOT1

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15f4e0b0e06a07eb8799ed142a9ff0b7
https://hdl.handle.net/1887/3184225

Conserved crosstalk between histone deacetylation and H3K79 methylation generates DOT1L-dose dependency in HDAC1-deficient thymic lymphoma

Autor: Tessy Korthout, A. F. Maarten Altelaar, Eliza Mari Kwesi-Maliepaard, Liesbeth Hoekman, Sjoerd Hendriks, Sander Palit, Thierry Schmidlin, Heinz Jacobs, Mir Farshid Alemdehy, Jan Hermen Dannenberg, Sjoerd Klarenbeek, Fred van Leeuwen, Cesare Lancini, Tibor van Welsem, Chelsea M. McLean, Hanneke Vlaming, Thom M Molenaar

Publikováno v: EMBO Journal, 38(14). Nature Publishing Group
The EMBO Journal
EMBO journal, 38(14):e101564. Wiley-Blackwell

DOT1L methylates histone H3K79 and is aberrantly regulated in MLL‐rearranged leukemia. Inhibitors have been developed to target DOT1L activity in leukemia, but cellular mechanisms that regulate DOT1L are still poorly understood. We have identified

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::114523b516214375ff4414868b0783ab
https://hdl.handle.net/1874/386558

Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism

Autor: Haico van Attikum, Kirsten van Harten, Iris J. E. Stulemeijer, Trey Ideker, Rohith Srivas, Fred van Leeuwen, Su Ming Sun, Farid El Oualid, Hanneke Vlaming, Tineke L. Lenstra, Dominique Morais, Tibor van Welsem, Huib Ovaa, Frank C. P. Holstege, Tessy Korthout, Reggy Ekkebus, Deepani W. Poramba-Liyanage, Thom M Molenaar

Publikováno v: Nucleic Acids Research
Nucleic acids research, 46(21), 11251-11261. Oxford University Press
Nucleic Acids Research, 46(21), 11251-11261

The histone methyltransferase Dot1 is conserved from yeast to human and methylates lysine 79 of histone H3 (H3K79) on the core of the nucleosome. H3K79 methylation by Dot1 affects gene expression and the response to DNA damage, and is enhanced by mon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca968c97e6ee05a0158c1e40575b5132
https://hdl.handle.net/1887/86410