Behavioral Assays to Study Oxygen and Carbon Dioxide Sensing in Caenorhabditis elegans

Autor: Teresa Rojo Romanos, Leelee Ng, Manuel Zimmer, Roger Pocock

Publikováno v: Bio-Protocol, Vol 8, Iss 1 (2018)

Animals use behavioral strategies to seek optimal environments. Population behavioral assays provide a robust means to determine the effect of genetic perturbations on the ability of animals to sense and respond to changes in the environment. Here, w

Externí odkaz: https://doaj.org/article/3d405d22e23c42f3b9c9157b14415a09

EGL-13/SoxD specifies distinct O2 and CO2 sensory neuron fates in Caenorhabditis elegans.

Autor: Jakob Gramstrup Petersen, Teresa Rojo Romanos, Vaida Juozaityte, Alba Redo Riveiro, Ingrid Hums, Lisa Traunmüller, Manuel Zimmer, Roger Pocock

Publikováno v: PLoS Genetics, Vol 9, Iss 5, p e1003511 (2013)

Animals harbor specialized neuronal systems that are used for sensing and coordinating responses to changes in oxygen (O2) and carbon dioxide (CO2). In Caenorhabditis elegans, the O2/CO2 sensory system comprises functionally and morphologically disti

Externí odkaz: https://doaj.org/article/40fa7dc3516a4b30b6b648f313aaea6b

Correction of a Factor VIII genomic inversion with designer-recombinases

Autor: Felix Lansing, Liliya Mukhametzyanova, Teresa Rojo-Romanos, Kentaro Iwasawa, Masaki Kimura, Maciej Paszkowski-Rogacz, Janet Karpinski, Tobias Grass, Jan Sonntag, Paul Martin Schneider, Ceren Günes, Jenna Hoersten, Lukas Theo Schmitt, Natalia Rodriguez-Muela, Ralf Knöfler, Takanori Takebe, Frank Buchholz

Publikováno v: Nature Communications 13(1), 422 (2022). doi:10.1038/s41467-022-28080-7
Nature Communications
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)

Despite advances in nuclease-based genome editing technologies, correcting human disease-causing genomic inversions remains a challenge. Here, we describe the potential use of a recombinase-based system to correct the 140 kb inversion of the F8 gene

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdb9b5cf670a12c5b81e8e93ee0407cf
https://doi.org/10.1038/s41467-022-28080-7

A heterodimer of evolved designer-recombinases precisely excises a human genomic DNA locus

Autor: Jan Sonntag, Felix Lansing, Teresa Rojo Romanos, Paul Martin Schneider, Lukas Theo Schmitt, Maciej Paszkowski-Rogacz, Frank Buchholz

Publikováno v: Nucleic Acids Research

Site-specific recombinases (SSRs) such as the Cre/loxP system are useful genome engineering tools that can be repurposed by altering their DNA-binding specificity. However, SSRs that delete a natural sequence from the human genome have not been repor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18c6e8dc67ef6ec845598085d10855f5

LIN-32/Atonal Controls Oxygen Sensing Neuron Development in Caenorhabditis elegans

Autor: Roger Pocock, David Pladevall-Morera, Leelee Ng, Teresa Rojo Romanos, Stine Thestrup Hansen, Kasper Langebeck-Jensen

Publikováno v: Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Romanos, T R, Pladevall-Morera, D, Langebeck-Jensen, K, Hansen, S, Ng, L & Pocock, R 2017, ' LIN-32/Atonal Controls Oxygen Sensing Neuron Development in Caenorhabditis elegans ', Scientific Reports, vol. 7, no. 1, 7294 . https://doi.org/10.1038/s41598-017-07876-4

Development of complex nervous systems requires precisely controlled neurogenesis. The generation and specification of neurons occur through the transcriptional and post-transcriptional control of complex regulatory networks. In vertebrates and inver

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db60a9d413c88974302f24a94caf0c91
https://doi.org/10.1038/s41598-017-07876-4