Actin Microfilaments Facilitate the Retrograde Transport from the Golgi Complex to the Endoplasmic Reticulum in Mammalian Cells

Autor: Ferran Valderrama, Teresa Babia, Jaime Renau-Piqueras, Holger Barth, Juan M. Duran, Gustavo Egea

Publikováno v: Traffic. 2:717-726

The morphology and subcellular positioning of the Golgi complex depend on both microtubule and actin cytoskeletons. In contrast to microtubules, the role of actin cytoskeleton in the secretory pathway in mammalian cells has not been clearly establish

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1ea8e84f8e90a53e83b78675a7bb0add
https://doi.org/10.1034/j.1600-0854.2001.21006.x

Morphological changes in the Golgi complex correlate with actin cytoskeleton rearrangements

Autor: M. A. De Matteis, Teresa Babia, Ferran Valderrama, Gustavo Egea, Alberto Luini, A. Di Campli

Publikováno v: Cell Motility and the Cytoskeleton. 43:334-348

In this report we have studied the morphological changes of the Golgi complex (GC) that specifically accompany F-actin reorganizations. In starved rat RBL-2H3 tumor mast cells, the GC, that was visualized at immunofluorescence level with antibodies r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b9afd4221c6dec98b67808443bf4643
https://doi.org/10.1002/(sici)1097-0169(1999)43:4<334::aid-cm6>3.0.co

PDMP blocks brefeldin A-induced retrograde membrane transport from Golgi to ER

Autor: Jan Willem Kok, Gustavo Egea, Catalin M. Filipeanu, Adriaan Nelemans, Dick Hoekstra, Teresa Babia

Publikováno v: Journal of Cell Biology, 142(1), 25-38. ROCKEFELLER UNIV PRESS
Recercat. Dipósit de la Recerca de Catalunya
instname
The Journal of Cell Biology
Dipòsit Digital de la UB
Universidad de Barcelona

In this study, we show that an inhibitor of sphingolipid biosynthesis, d,l-threo-1-phenyl-2- decanoylamino-3-morpholino-1-propanol (PDMP), inhibits brefeldin A (BFA)-induced retrograde membrane transport from Golgi to endoplasmic reticulum (ER). If B

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7735d2f6c2fedc3518b6a1f9bebe145c
https://hdl.handle.net/11370/51b8c91c-407a-496c-a585-716ad20cdc8b

The golgi-associated COPI-coated buds and vesicles contain beta/gamma -actin

Autor: Teresa Babia, José Ballesta, Ana Luna, Holger Barth, Jaime Renau-Piqueras, Ferran Valderrama, José A. Martínez-Menárguez, Christine Chaponnier, Gustavo Egea

Publikováno v: Proceedings of the National Academy of Sciences, Vol. 97, No 4 (2000) pp. 1560-1565

It has been shown previously that the morphology and subcellular positioning of the Golgi complex is controlled by actin microfilaments. To further characterize the association between actin microfilaments and the Golgi complex, we have used the Clos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71f966e10ecac1e6956f39d16fe95cb8
https://pubmed.ncbi.nlm.nih.gov/10677499

Immunofluorescence and Immunoelectron Microscopy of Microinjected and Transfected Cultured Cells

Autor: Roser Pagan, Teresa Babia, Roser Buscà, Senén Vilaró, Gustavo Egea, Manuel Reina, Inmaculada Ayala, Ferran Valderrama

Publikováno v: Microinjection and Transgenesis ISBN: 9783540618959

Immunofluorescence microscopy is the most common method to analyze expression and localization of a given protein in cells that have been microinjected or transfected previously with the appropriate DNA constructs. It offers the advantage that it is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::de4f765576103c4367702ce4d473db99
https://doi.org/10.1007/978-3-642-80343-7_3