Výsledky vyhledávání - "Terence D Allen"

NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes

Autor: Christopher J. Hutchison, Juliet A. Ellis, Georgia Salpingidou, Elena Kiseleva, Anja Golebiewska, Sheona P Drummond, Ryszard Rzepecki, Kasia Fusiek, Carol E. Lyon, Martin W. Goldberg, Birgit Lane, Carl Smythe, Terence D Allen

Publikováno v: Journal of Cell Science. 121:706-716

In vertebrates, the nuclear envelope (NE) assembles and disassembles during mitosis. As the NE is a complex structure consisting of inner and outer membranes, nuclear pore complexes (NPCs) and the nuclear lamina, NE assembly must be a controlled and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3018bfbf45484f907460e1310e7d64a
https://doi.org/10.1242/jcs.019968

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A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM)

Autor: K. N. Morozova, Elena Kiseleva, Fiona Gardiner, Terence D Allen, Martin W. Goldberg, Sandra A Rutherford, Stephen M Murray, Sheona P Drummond

Publikováno v: Nature Protocols. 2:1943-1953

This protocol details methods for the isolation of yeast nuclei from budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe), immuno-gold labeling of proteins and visualization by field emission scanning electron micros

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9065eb678df4125afa7180933158f4b3
https://doi.org/10.1038/nprot.2007.251

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A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM)

Autor: Fiona Gardiner, Terence D Allen, Helen S Sanderson, Martin W. Goldberg, Sandra A Rutherford, Stephen M Murray, Sheona P Drummond, Elena Kiseleva

Publikováno v: Nature Protocols. 2:1166-1172

This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African clawed toad Xenopus laevis, immunogold labeling of component proteins and subsequent visualization by field-emission scanning electron microscopy (FESE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f5b288a6ba4889c3dd12a1693649573
https://doi.org/10.1038/nprot.2007.137

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Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM)

Autor: Sheona P Drummond, Terence D Allen, Sandra A Rutherford, Stephen M Murray, Martin W. Goldberg, Fiona Gardiner, Elena Kiseleva, Helen S Sanderson

Publikováno v: Nature Protocols. 2:1173-1179

This protocol details methods for the generation of cell-free extracts and DNA templates from the eggs and sperm chromatin, respectively, of the clawed toad Xenopus laevis. We have used this system with scanning electron microscopy (SEM), as detailed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aeca550488d33b917295640361d567c4
https://doi.org/10.1038/nprot.2007.138

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Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells

Autor: Sheona P Drummond, Terence D Allen, Sandra A Rutherford, Stephen M Murray, Fiona Gardiner, Martin W. Goldberg, Elena Kiseleva

Publikováno v: Nature Protocols. 2:1180-1184

Our previous work characterizing the biogenesis and structural integrity of the nuclear envelope and nuclear pore complexes (NPCs) has been based on amphibian material but has recently progressed into the analysis of tissue-culture cells. This protoc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85bf3bc3aea9ab97c7e61fdc06e355ab
https://doi.org/10.1038/nprot.2007.139

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Actin- and protein-4.1-containing filaments link nuclear pore complexes to subnuclear organelles inXenopusoocyte nuclei

Autor: Sheona P Drummond, Katherine L. Wilson, Sandra A Rutherford, Elena Kiseleva, Martin W. Goldberg, Terence D Allen

Publikováno v: Journal of Cell Science. 117:2481-2490

We imaged the interiors of relatively intact Xenopus oocyte nuclei by field emission scanning electron microscopy (feSEM) and visualized a network of filaments that attach to nuclear pore complexes and extend throughout the nucleus. Within the nucleu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51c7f70c8eb06730233ca993c77808ba
https://doi.org/10.1242/jcs.01098

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Electrochemical Monitoring of Anticancer Compounds on the Human Ovarian Carcinoma Cell Line A2780 and Its Adriamycin- and Cisplatin-Resistant Variants

Autor: L. C. Tetlow, Terence D Allen, David E. Woolley, Robert David Eden, David Gearey, Timothy H Ward, Daman J. Adlam

Publikováno v: Experimental Cell Research. 273:65-72

A novel electrochemical technique which detects and monitors real-time changes in cell behavior in vitro has been used to examine the effects of recognized anticancer drugs on the human ovarian carcinoma cell line A2780 and its adriamycin (A2780adr)-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be673710b923375fa90e60ca1371acbd
https://doi.org/10.1006/excr.2001.5428

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The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins

Autor: Helen Pickersgill, Tobias C. Walther, Maarten Fornerod, Terence D Allen, Iain W. Mattaj, Martin W. Goldberg

Publikováno v: The EMBO Journal. 20:5703-5714

The nuclear pore complex (NPC) is a large proteinaceous structure through which bidirectional transport of macromolecules across the nuclear envelope (NE) takes place. Nup153 is a peripheral NPC component that has been implicated in protein and RNP t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d647172566da6909b386e07c8df25259
https://doi.org/10.1093/emboj/20.20.5703

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Ran alters nuclear pore complex conformation

Autor: Laura A Cotter, Steven Bagley, Paul Clarke, Elena Kiseleva, Martin W. Goldberg, Mike F. Hughes, Terence D Allen, Sandra A Rutherford

Publikováno v: Journal of Molecular Biology. 300:519-529

Transport across the nuclear membranes occurs through the nuclear pore complex (NPC), and is mediated by soluble transport factors including Ran, a small GTPase that is generally GDP-bound during import and GTP-bound for export. The dynamic nature of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7786118694f89530ff3b30a9ffc06a2b
https://doi.org/10.1006/jmbi.2000.3891

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