Mutant huntingtin impairs neurodevelopment in human brain organoids through CHCHD2-mediated neurometabolic failure.

Autor: Lisowski P; Quantitative Stem Cell Biology, Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany.; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; Department of Psychiatry and Psychotherapy, Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité - Universitätsmedizin, Berlin, Germany.; Department of Molecular Biology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec n/Warsaw, Poland., Lickfett S; Faculty of Mathematics and Natural Sciences, Heinrich Heine University, Düsseldorf, Germany.; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital Düsseldorf, Heinrich Heine University, Düsseldorf, Germany.; Institute of Anatomy II, Heinrich-Heine-University, Düsseldorf, Germany., Rybak-Wolf A; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; Organoid Platform, Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany., Menacho C; Faculty of Mathematics and Natural Sciences, Heinrich Heine University, Düsseldorf, Germany.; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital Düsseldorf, Heinrich Heine University, Düsseldorf, Germany., Le S; Faculty of Mathematics and Natural Sciences, Heinrich Heine University, Düsseldorf, Germany.; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital Düsseldorf, Heinrich Heine University, Düsseldorf, Germany., Pentimalli TM; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; Laboratory for Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany.; Charité - Universitätsmedizin, Berlin, Germany., Notopoulou S; Institute of Applied Biosciences (INAB), Centre For Research and Technology Hellas (CERTH), Thessaloniki, Greece., Dykstra W; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; Department of Translational Neuroscience, University Medical Center Utrecht Brain Center, Utrecht, The Netherlands., Oehler D; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty and University Hospital Düsseldorf, Cardiovascular Research Institute Düsseldorf (CARID), Düsseldorf, Germany., López-Calcerrada S; Instituto de Investigación Hospital 12 de Octubre (i + 12), Madrid, Spain., Mlody B; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; Centogene, Rostock, Germany., Otto M; Quantitative Stem Cell Biology, Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany.; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Wu H; Institute of Molecular Medicine, Medical School, Hamburg, Germany., Richter Y; Cell Biology, University of Bremen, Bremen, Germany., Roth P; Quantitative Stem Cell Biology, Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany.; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Anand R; Institute of Biochemistry and Molecular Biology I, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University, Düsseldorf, Germany., Kulka LAM; Institute of Physiological Chemistry, Martin-Luther-University, Halle-Wittenberg, Germany., Meierhofer D; Quantitative RNA Biology, Max Planck Institute for Molecular Genetics, Berlin, Germany., Glazar P; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; Laboratory for Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany.; Quantitative RNA Biology, Max Planck Institute for Molecular Genetics, Berlin, Germany., Legnini I; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; Laboratory for Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany.; Human Technopole, Milan, Italy., Telugu NS; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Hahn T; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Neuendorf N; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Miller DC; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Böddrich A; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Polzin A; Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty and University Hospital Düsseldorf, Cardiovascular Research Institute Düsseldorf (CARID), Düsseldorf, Germany., Mayatepek E; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital Düsseldorf, Heinrich Heine University, Düsseldorf, Germany., Diecke S; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; German Center for Cardiovascular Research (DZHK), Berlin, Germany., Olzscha H; Institute of Molecular Medicine, Medical School, Hamburg, Germany.; Institute of Physiological Chemistry, Martin-Luther-University, Halle-Wittenberg, Germany., Kirstein J; Cell Biology, University of Bremen, Bremen, Germany.; Leibniz Institute on Aging - Fritz-Lipmann Institute, Jena, Germany., Ugalde C; Instituto de Investigación Hospital 12 de Octubre (i + 12), Madrid, Spain.; Centro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), Madrid, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, Spain., Petrakis S; Institute of Applied Biosciences (INAB), Centre For Research and Technology Hellas (CERTH), Thessaloniki, Greece., Cambridge S; Institute of Anatomy II, Heinrich-Heine-University, Düsseldorf, Germany.; Dr. Senckenberg Anatomy, Anatomy II, Goethe-University, Frankfurt, Germany., Rajewsky N; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; Laboratory for Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany.; German Center for Cardiovascular Research (DZHK), Berlin, Germany.; NeuroCure Cluster of Excellence, Berlin, Germany.; National Center for Tumor Diseases (NCT), German Cancer Consortium (DKTK), Berlin, Germany.; German Center for Neurodegenerative Diseases (DZNE), Berlin, Germany., Kühn R; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Wanker EE; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Priller J; Department of Psychiatry and Psychotherapy, Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité - Universitätsmedizin, Berlin, Germany.; German Center for Neurodegenerative Diseases (DZNE), Berlin, Germany.; Department of Psychiatry and Psychotherapy; School of Medicine and Health, Technical University of Munich and German Center for Mental Health (DZPG), Munich, Germany.; University of Edinburgh and UK Dementia Research Institute, Edinburgh, UK., Metzger JJ; Quantitative Stem Cell Biology, Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany. jakob.metzger@mdc-berlin.de.; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany. jakob.metzger@mdc-berlin.de., Prigione A; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany. alessandro.prigione@hhu.de.; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital Düsseldorf, Heinrich Heine University, Düsseldorf, Germany. alessandro.prigione@hhu.de.

Publikováno v: Nature communications [Nat Commun] 2024 Aug 22; Vol. 15 (1), pp. 7027. Date of Electronic Publication: 2024 Aug 22.

Phosphatidylinositol 3,5-bisphosphate facilitates axonal vesicle transport and presynapse assembly.

Autor: Rizalar FS; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany., Lucht MT; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany., Petzoldt A; Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany., Kong S; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany., Sun J; Department of Chemistry, University of Illinois Chicago, Chicago, IL 60607, USA., Vines JH; School of Biosciences, University of Sheffield, Firth Court Western Bank, Sheffield S10 2TN, UK., Telugu NS; Max-Delbrück-Centrum für Molekulare Medizin (MDC), Technology Platform Pluripotent Stem Cells, 13125 Berlin, Germany., Diecke S; Max-Delbrück-Centrum für Molekulare Medizin (MDC), Technology Platform Pluripotent Stem Cells, 13125 Berlin, Germany., Kaas T; Carl-Ludwig Institute of Physiology, Faculty of Medicine, Leipzig University, 04103 Leipzig, Germany., Bullmann T; Carl-Ludwig Institute of Physiology, Faculty of Medicine, Leipzig University, 04103 Leipzig, Germany., Schmied C; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany., Löwe D; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany., King JS; School of Biosciences, University of Sheffield, Firth Court Western Bank, Sheffield S10 2TN, UK., Cho W; Department of Chemistry, University of Illinois Chicago, Chicago, IL 60607, USA., Hallermann S; Carl-Ludwig Institute of Physiology, Faculty of Medicine, Leipzig University, 04103 Leipzig, Germany., Puchkov D; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany., Sigrist SJ; Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany., Haucke V; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany.; Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany.; NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.

Publikováno v: Science (New York, N.Y.) [Science] 2023 Oct 13; Vol. 382 (6667), pp. 223-230. Date of Electronic Publication: 2023 Oct 12.

High-content analysis of neuronal morphology in human iPSC-derived neurons.

Autor: Lickfett S; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Department of Anatomy II, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany., Menacho C; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany., Zink A; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany., Telugu NS; Max Delbrück Center for Molecular Medicine (MDC) and Berlin Institute of Health (BIH), 13125 Berlin, Germany., Beller M; Institute for Mathematical Modeling of Biological Systems, Heinrich Heine University, 40225 Düsseldorf, Germany., Diecke S; Max Delbrück Center for Molecular Medicine (MDC) and Berlin Institute of Health (BIH), 13125 Berlin, Germany., Cambridge S; Department of Anatomy II, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Dr. Senckenbergische Anatomie, Goethe-Universität Frankfurt, 60590 Frankfurt am Main, Germany., Prigione A; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany. Electronic address: alessandro.prigione@hhu.de.

Publikováno v: STAR protocols [STAR Protoc] 2022 Aug 06; Vol. 3 (3), pp. 101567. Date of Electronic Publication: 2022 Aug 06 (Print Publication: 2022).

Methods for Automated Single Cell Isolation and Sub-Cloning of Human Pluripotent Stem Cells.

Autor: Vallone VF; Charité-Universitätsmedizin Berlin, Berlin, Germany.; Berlin Institute of Health (BIH), BIH Stem Cell Core Facility, Berlin, Germany., Telugu NS; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; BIH Stem Cell Core Facility, Berlin Institute of Health (BIH), Berlin, Germany., Fischer I; Charité-Universitätsmedizin Berlin, Berlin, Germany.; Berlin Institute of Health (BIH), BIH Stem Cell Core Facility, Berlin, Germany., Miller D; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; BIH Stem Cell Core Facility, Berlin Institute of Health (BIH), Berlin, Germany.; DZHK (German Centre for Cardiovascular Research), partner site Berlin, Berlin, Germany., Schommer S; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; BIH Stem Cell Core Facility, Berlin Institute of Health (BIH), Berlin, Germany., Diecke S; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; BIH Stem Cell Core Facility, Berlin Institute of Health (BIH), Berlin, Germany.; DZHK (German Centre for Cardiovascular Research), partner site Berlin, Berlin, Germany., Stachelscheid H; Charité-Universitätsmedizin Berlin, Berlin, Germany.; Berlin Institute of Health (BIH), BIH Stem Cell Core Facility, Berlin, Germany.

Publikováno v: Current protocols in stem cell biology [Curr Protoc Stem Cell Biol] 2020 Dec; Vol. 55 (1), pp. e123.

CPAP promotes timely cilium disassembly to maintain neural progenitor pool.

Autor: Gabriel E; Center for Molecular Medicine and Institute for Biochemistry I of the University of Cologne, Cologne, Germany., Wason A; Center for Molecular Medicine and Institute for Biochemistry I of the University of Cologne, Cologne, Germany., Ramani A; Center for Molecular Medicine and Institute for Biochemistry I of the University of Cologne, Cologne, Germany., Gooi LM; Center for Molecular Medicine and Institute for Biochemistry I of the University of Cologne, Cologne, Germany., Keller P; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany., Pozniakovsky A; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany., Poser I; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany., Noack F; DFG-Research Center and Cluster of Excellence for Regenerative Therapies, TU-Dresden, Dresden, Germany., Telugu NS; Center for Physiology and Pathophysiology, Institute for Neurophysiology Medical Faculty University of Cologne, Cologne, Germany., Calegari F; DFG-Research Center and Cluster of Excellence for Regenerative Therapies, TU-Dresden, Dresden, Germany., Šarić T; Center for Physiology and Pathophysiology, Institute for Neurophysiology Medical Faculty University of Cologne, Cologne, Germany., Hescheler J; Center for Physiology and Pathophysiology, Institute for Neurophysiology Medical Faculty University of Cologne, Cologne, Germany., Hyman AA; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany., Gottardo M; Department of Life Sciences, University of Siena, Siena, Italy., Callaini G; Department of Life Sciences, University of Siena, Siena, Italy., Alkuraya FS; Department of Genetics, King Faisal Specialist Hospital and Research Center Alfasial University, Riyadh, Saudi Arabia Department of Anatomy and Cell Biology, College of Medicine Alfasial University, Riyadh, Saudi Arabia., Gopalakrishnan J; Center for Molecular Medicine and Institute for Biochemistry I of the University of Cologne, Cologne, Germany jay.gopalakrishnan@uni-koeln.de.

Publikováno v: The EMBO journal [EMBO J] 2016 Apr 15; Vol. 35 (8), pp. 803-19. Date of Electronic Publication: 2016 Feb 29.