Spatial separation of Xist RNA and polycomb proteins revealed by superresolution microscopy

Autor: Cerase A, Smeets D, Amy Tang, Gdula M, Kraus F, Spivakov M, Moindrot B, Leleu M, Tattermusch A, Demmerle J, Tb, Nesterova, Green C, Ap, Otte, Schermelleh L, Brockdorff N

Publikováno v: Europe PubMed Central

In female mammals, one of the two X chromosomes is transcriptionally silenced to equalize X-linked gene dosage relative to XY males, a process termed X chromosome inactivation. Mechanistically, this is thought to occur via directed recruitment of chr

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Xist-mediated silencing requires additive functions of SPEN and Polycomb together with differentiation-dependent recruitment of SmcHD1.

Autor: Bowness JS; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Nesterova TB; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Wei G; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Rodermund L; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Almeida M; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Coker H; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Carter EJ; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Kadaster A; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Brockdorff N; Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address: neil.brockdorff@bioch.ox.ac.uk.

Publikováno v: Cell reports [Cell Rep] 2022 May 17; Vol. 39 (7), pp. 110830.

Systematic allelic analysis defines the interplay of key pathways in X chromosome inactivation.

Autor: Nesterova TB; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Wei G; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Coker H; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Pintacuda G; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.; Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA, 02138, USA., Bowness JS; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Zhang T; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Almeida M; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Bloechl B; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Moindrot B; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.; I2BC Paris-Sud University, Gif-Sur-Yvette, France., Carter EJ; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Alvarez Rodrigo I; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.; Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK., Pan Q; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Bi Y; Ludwig Institute for Cancer Research, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7FZ, UK., Song CX; Ludwig Institute for Cancer Research, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7FZ, UK., Brockdorff N; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK. neil.brockdorff@bioch.ox.ac.uk.

Publikováno v: Nature communications [Nat Commun] 2019 Jul 16; Vol. 10 (1), pp. 3129. Date of Electronic Publication: 2019 Jul 16.

hnRNPK Recruits PCGF3/5-PRC1 to the Xist RNA B-Repeat to Establish Polycomb-Mediated Chromosomal Silencing.

Autor: Pintacuda G; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Wei G; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Roustan C; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Kirmizitas BA; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Solcan N; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Cerase A; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Castello A; Posttranscriptional Networks in Infection and Cell Cycle Progression, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Mohammed S; Proteomics Technology Development and Application, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Moindrot B; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Nesterova TB; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Brockdorff N; Developmental Epigenetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address: neil.brockdorff@bioch.ox.ac.uk.

Publikováno v: Molecular cell [Mol Cell] 2017 Dec 07; Vol. 68 (5), pp. 955-969.e10.

The nuclear matrix protein CIZ1 facilitates localization of Xist RNA to the inactive X-chromosome territory.

Autor: Ridings-Figueroa R; Department of Biology, University of York, York YO10 5DD, United Kingdom., Stewart ER; Department of Biology, University of York, York YO10 5DD, United Kingdom., Nesterova TB; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Coker H; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Pintacuda G; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Godwin J; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Wilson R; Department of Biology, University of York, York YO10 5DD, United Kingdom., Haslam A; Department of Biology, University of York, York YO10 5DD, United Kingdom., Lilley F; Department of Biology, University of York, York YO10 5DD, United Kingdom., Ruigrok R; Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, Leeds LS2 9JT, United Kingdom., Bageghni SA; Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, Leeds LS2 9JT, United Kingdom., Albadrani G; Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, Leeds LS2 9JT, United Kingdom.; Princess Nourah Bint Abdulrahman University (PNU), Riyadh, Kingdom of Saudi Arabia., Mansfield W; Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, United Kingdom., Roulson JA; Leeds Institute of Molecular Medicine (LIMM), University of Leeds, Leeds LS9 7TF, United Kingdom., Brockdorff N; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Ainscough JFX; Department of Biology, University of York, York YO10 5DD, United Kingdom.; Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, Leeds LS2 9JT, United Kingdom., Coverley D; Department of Biology, University of York, York YO10 5DD, United Kingdom.

Publikováno v: Genes & development [Genes Dev] 2017 May 01; Vol. 31 (9), pp. 876-888. Date of Electronic Publication: 2017 May 25.