Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Tatsuyuki Yoshii"'
Publikováno v:
STAR Protocols, Vol 3, Iss 2, Pp 101451- (2022)
Summary: Here, we describe a protocol for the translational regulation of transfected messenger RNAs (mRNAs) using light in mammalian cells. We detail the steps for photocaged ligand synthesis, template DNA preparation, and mRNA synthesis. We describ
Externí odkaz:
https://doaj.org/article/17a3ccfc560b47e2984cdbe9345db45a
Publikováno v:
Bioorganicmedicinal chemistry letters. 72
Light-mediated control of protein localization in living cells is a powerful approach for manipulating and probing complex biological systems. By incorporating a classical 6-nitroveratryloxycarbonyl (NVOC) caging group into the inner plasma membrane
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0fec15a912d1ed791ffeff013f1da06
https://pubmed.ncbi.nlm.nih.gov/35738351
https://pubmed.ncbi.nlm.nih.gov/35738351
Autor:
Shunsuke Sawada, Akinobu Nakamura, Kentaro Noma, Choji Oki, Andrew K. Rudd, Shinya Tsukiji, Neal K. Devaraj, Tatsuyuki Yoshii, Keiko Kuwata
Publikováno v:
ACS Chemical Biology. 15:837-843
Inducing protein translocation to the plasma membrane (PM) is an important approach for manipulating diverse signaling molecules/pathways in living cells. We previously devised a new chemogenetic system, in which a protein fused to Escherichia coli d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2bf0b3cce5734ba0a41d6902ed588fa9
https://doi.org/10.1021/acschembio.0c00014
https://doi.org/10.1021/acschembio.0c00014
Autor:
Shunsuke Sawada, Shinya Tsukiji, Akinobu Nakamura, Fubito Nakatsu, Keiko Kuwata, Tatsuyuki Yoshii
Publikováno v:
Chemical Communications. 56:15422-15425
Organelle-localizable small-molecule ligands are valuable tools for spatiotemporally controlling protein localization and signaling processes in living cells. Here, we present synthetic ligands that specifically localize to the Golgi surface. The lig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fce487a720d6513d0402d528e2b3b1c0
https://doi.org/10.1039/d0cc06908f
https://doi.org/10.1039/d0cc06908f
Autor:
Akinobu Nakamura, Tatsuyuki Yoshii, Keiko Kuwata, Rei Watahiki, Shinya Tsukiji, Choji Oki, Toshiaki Furuta, Kai Tahara
Publikováno v:
ACS chemical biology. 16(8)
Manipulating subcellular protein localization using light is a powerful approach for controlling signaling processes with high spatiotemporal precision. The most widely used strategy for this is based on light-induced protein heterodimerization. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a79f0bfed45c0eaf31ec8111a1b81ca8
https://pubmed.ncbi.nlm.nih.gov/34339163
https://pubmed.ncbi.nlm.nih.gov/34339163
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2312
Chemical control of protein localization is a powerful approach for manipulating mammalian cellular processes. Self-localizing ligand-induced protein translocation (SLIPT) is an emerging platform that enables control of protein localization in living
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bbfaf4f973a104c9e5e85ee86366f4af
https://pubmed.ncbi.nlm.nih.gov/34228294
https://pubmed.ncbi.nlm.nih.gov/34228294
Autor:
Eri Shinoda, Keiko Kuwata, Tatsuyuki Yoshii, Shinya Tsukiji, Shunsuke Sawada, Akinobu Nakamura, Rika Katahira
Publikováno v:
Biochemistry. 59:205-211
The Self-localizing Ligand-Induced Protein Translocation(SLIPT) system is an emerging platform that controls protein localization in living cells using synthetic self-localizing ligands (SLs). Here, we report a chemogenetic SLIPT system for inducing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93749ef00e520bf7936da67e5ec2d2d9
https://doi.org/10.1021/acs.biochem.9b00807
https://doi.org/10.1021/acs.biochem.9b00807
Publikováno v:
Journal of the American Chemical Society. 143(17)
Compartmentation of proteins into biomolecular condensates or membraneless organelles formed by phase separation is an emerging principle for the regulation of cellular processes. Creating synthetic condensates that accommodate specific intracellular
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3044370918f15bf87b1c2d1f6a00752e
https://pubmed.ncbi.nlm.nih.gov/33890764
https://pubmed.ncbi.nlm.nih.gov/33890764
Autor:
Shinya Tsukiji, Tatsuyuki Yoshii, Yuka Hatano, Sachio Suzuki, Atsuta-Tsunoda K, Akinobu Nakamura, Kazuhiro Aoki
Publikováno v:
Cell chemical biology. 29(9)
Chemogenetic methods that enable the rapid translocation of specific signaling proteins in living cells using small molecules are powerful tools for manipulating and interrogating intracellular signaling networks. However, existing techniques rely on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abded5a86b3996ff9b99cbb63d5d49f9
https://pubmed.ncbi.nlm.nih.gov/35835118
https://pubmed.ncbi.nlm.nih.gov/35835118
Publikováno v:
Methods in Molecular Biology ISBN: 9781071614402
Chemical control of protein localization is a powerful approach for manipulating mammalian cellular processes. Self-localizing ligand-induced protein translocation (SLIPT) is an emerging platform that enables control of protein localization in living
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::092ad31c99823febac4f417c933c9682
https://doi.org/10.1007/978-1-0716-1441-9_14
https://doi.org/10.1007/978-1-0716-1441-9_14
