Zobrazeno 1 - 10
of 112
pro vyhledávání: '"Tatsuji Seki"'
Publikováno v:
Plant Biotechnology. 29:489-493
Suspension-cultured plant cells are feasible bioreactors for the production of pharmaceutical proteins. Posttranslational modification, such as glycosylation, is important for in vivo biological and physiological roles of proteins. However, little is
Publikováno v:
Journal of Bioscience and Bioengineering. 111:471-477
Human CMP-N-acetylneuraminic acid (NeuAc) synthase (hCSS) and α2,6-sialyltransferase (hST) participate in the sialylation of N-linked glycans in mammalian cells. hCSS synthesizes CMP-NeuAc, which hST uses as a donor substrate to transfer NeuAc to th
Publikováno v:
Glycobiology. 20:736-751
The core oligosaccharide Glc(3)Man(9)GlcNAc(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (ER). The first step of this assembly pathway on the
Publikováno v:
Journal of Bioscience and Bioengineering. 107:165-172
The production of antibodies using plants as bioreactors has been realized. Because sugar chain structures on recombinant proteins are a cause of concern, remodeling technology is highly promising. Localizing recombinant proteins in the endoplasmic r
Publikováno v:
Mycoscience. 49:258-265
The molecular phylogeny of nuclear LSU rDNA sequences (D1/D2 domain), ITS regions, and beta-tubulin gene (tub2) showed that the seven strains of Shiraia-like fungi obtained from fresh bamboo tissues as endophytes were closely related to Shiraia bambu
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 72:1416-1429
A polyphasic study was performed to determine the taxonomic position of strain EK05(T) isolated from a root-outgrowth of Entada koshunensis, a legume available in Okinawa, Japan. Phylogenetic analysis of the 16S rRNA gene showed that the strain belon
Autor:
Seiji Ishiyama, Toshiki Tamura, Hideki Sezutsu, Tatsuji Seki, Kazuei Mita, Kazuhito Fujiyama, Akihiro Usami, Takahiro Okada
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 71:1626-1635
Beta-N-acetylglucosaminidase is a major glycosidase involved in several physiological processes, such as fertilization, metamorphosis, glycoconjugate degradation, and glycoprotein biosynthesis in insects. A search using the Bombyx mori cDNA database
Publikováno v:
Enzyme and Microbial Technology. 40:1221-1227
The gene encoding sucrose isomerase ( pal I NK33) was cloned from Klebsiella pneumoniae strain NK33-98-8. The gene was over-expressed in Escherichia coli BL21 (DE3) pLysS and its enzyme product (PalI NK33) was purified and characterized. PalI NK33 co
Publikováno v:
Plant Biotechnology. 24:255-259
The oligosaccharide structures of total glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells are determined. The N-linked sugar chains released by hydrazinolysis were labeled with 2-aminopyridine, and analyzed by a combination of HPLC
Publikováno v:
Archives of Microbiology. 186:327-337
In the present study, the transcriptional properties of the nitrogen fixation gene cluster of Hbt. chlorum, a strictly anaerobic, gram-positive, phototrophic bacterium, were explored. The cluster consisted of eleven genes in the same orientation in t