Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Taryn L. O'Loughlin"'
Autor:
Ichiro Matsumura, Taryn L. O'Loughlin
Publikováno v:
Combinatorial Chemistry & High Throughput Screening. 9:313-320
Our long-term goal is to direct the evolution of novel protease variants. To this end we have engineered a new type of protease-activated reporter enzyme. Many protease-activated enzymes evolved in nature, but the introduction of novel regulatory mec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae9f82904e8ee5ac60d769235d1392f5
https://doi.org/10.2174/138620706776843219
https://doi.org/10.2174/138620706776843219
Publikováno v:
Protein engineering, designselection : PEDS. 19(10)
Natural selection generally produces specific and efficient enzymes. In contrast, directed evolution experiments usually produce enzyme variants with broadened substrate specificity or enhanced catalytic promiscuity. Some proteins may be more evolvab
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd102edb2b991b18ba5735b1c917d75c
https://pubmed.ncbi.nlm.nih.gov/16868005
https://pubmed.ncbi.nlm.nih.gov/16868005
Publikováno v:
Molecular biology and evolution. 23(4)
Our goal is to understand how enzymes adapt to utilize novel substrates. We and others have shown that directed evolution tends to generate enzyme variants with broadened substrate specificity. Broad-specificity enzymes are generally deleterious to l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58213cf4271dc919039875ba047768d8
https://pubmed.ncbi.nlm.nih.gov/16423863
https://pubmed.ncbi.nlm.nih.gov/16423863
Publikováno v:
The Journal of biological chemistry. 280(42)
The dominant paradigm of protein engineering is structure-based site-directed mutagenesis. This rational approach is generally more effective for the engineering of local properties, such as substrate specificity, than global ones such as allostery.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::454c6315f9a39e383e11f8a5d84d06aa
https://pubmed.ncbi.nlm.nih.gov/16118206
https://pubmed.ncbi.nlm.nih.gov/16118206