Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Tarmo Ruusala"'
Autor:
Tarmo Ruusala, Donald M. Crothers
Publikováno v:
Proceedings of the National Academy of Sciences. 89:4903-4907
The lac repressor associates with its operator at a rate faster than conventional diffusion allows, either because of one-dimensional diffusion of a captured repressor along the DNA (sliding) or because the tetrameric repressor can be rapidly transfe
Autor:
Tarmo Ruusala
Publikováno v:
Current Genetics. 20:379-383
The mating type of fission yeast is determined by the mat1 locus on chromosome II. The sequence content of this locus, and hence the mating type, is switched in a strictly regular pattern by transposition from one of two unexpressed mating type seque
Publikováno v:
Yeast. 7:745-755
The fission yeast, Schizosaccharomyces pombe, expresses one of two alternative mating types. They are specified by one of two determinants (M or P) present at the mat1 locus. In addition, silent copies of M and P are present on the same chromosome. I
Publikováno v:
Nucleic acids research. 21(4)
We show that in fission yeast two DNA fragments at the silent P mating type locus provide plasmids with the capability of autonomous replication. Bacterial vectors containing these sequences replicate in a polymeric form in fission yeast very much li
Trans-acting factors and properly positioned DNA elements repress mating-type genes in fission yeast
Publikováno v:
Current genetics. 21(4-5)
Repression of the mating-type P genes at the silent mat2-P locus in fission yeast is dependent on four cis-acting DNA elements, two on each side of the coding sequences. The mechanism by which these elements exert their influence on the mating-type p
Autor:
Tarmo Ruusala, Karl Ekwall
Publikováno v:
Nucleic Acids Research. 19:1150-1150
Publikováno v:
Biochimie. 68:261-273
Summary A new method (T. Ruusala et al., 1982, EMBO J. 1, 75–78, 741–748) for analyzing kinetic proofreading in translation is described. An in vitro system is arranged so that its rate of polypeptide synthesis is determined by the release rate o
Publikováno v:
Biochimie. 69:131-136
We have examined the interpretation that streptomycin kills a bacterial culture by initiating the so-called error catastrophe [8]. In particular, we asked whether the increased translational error rate induced by the antibiotic gives rise to an autoc
Autor:
Tarmo Ruusala, Jaroslav Weiser
Publikováno v:
FEBS Letters. (1):7-11
The method of purification of elongation factor Ts from Streptomyces aureofaciens is described. Purified elongation factors Ts from S. aureofaciens and Escherichia coli were tested in cross-reactivity studies with elongation factors Tu from both spec
Publikováno v:
The EMBO journal. 1(1)
The kinetic parameters which characterize the interaction between elongation factor Tu (EF-Tu) and elongation factor Ts (EF-Ts) have been determined in a poly(uridylic acid)-primed translation system. The EF-Ts catalyzed release of GDP from EF-Tu was