Targeted genomic enrichment and sequencing of CyHV-3 from carp tissues confirms low nucleotide diversity and mixed genotype infections

Autor: Saliha Hammoumi, Tatiana Vallaeys, Ayi Santika, Philippe Leleux, Ewa Borzym, Christophe Klopp, Jean-Christophe Avarre

Publikováno v: PeerJ, Vol 4, p e2516 (2016)

Koi herpesvirus disease (KHVD) is an emerging disease that causes mass mortality in koi and common carp, Cyprinus carpio L. Its causative agent is Cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus (KHV). Although data on the pathogenesis

Externí odkaz: https://doaj.org/article/79294bda4f1d457fb6c07422178200b2

Solution-based targeted genomic enrichment for precious DNA samples

Autor: Shearer Aiden, Hildebrand Michael S, Smith Richard JH

Publikováno v: BMC Biotechnology, Vol 12, Iss 1, p 20 (2012)

Abstract Background Solution-based targeted genomic enrichment (TGE) protocols permit selective sequencing of genomic regions of interest on a massively parallel scale. These protocols could be improved by: 1) modifying or eliminating time consuming

Externí odkaz: https://doaj.org/article/2320a0b8a6a04b8a8ff4181d5aa17f17

Genomic DNA Pooling Strategy for Next-Generation Sequencing-Based Rare Variant Discovery in Abdominal Aortic Aneurysm Regions of Interest—Challenges and Limitations

Autor: Wigard P. Kloosterman, Magdalena Harakalova, Annette F. Baas, Jelena Medic, Isaac J. Nijman, Michal Mokry, Ivo Renkens, Jan D. Blankensteijn, Edwin Cuppen

Publikováno v: Journal of Cardiovascular Translational Research, 4(3), 271-280. Springer New York
Harakalova, M, Nijman, I J, Medic, J, Mokry, M, Renkens, I, Blankensteijn, J D, Kloosterman, W, Baas, A F & Cuppen, E 2011, ' Genomic DNA Pooling Strategy for Next-Generation Sequencing-Based Rare Variant Discovery in Abdominal Aortic Aneurysm Regions of Interest-Challenges and Limitations ', Journal of Cardiovascular Translational Research, vol. 4, no. 3, pp. 271-280 . https://doi.org/10.1007/s12265-011-9263-5
Journal of Cardiovascular Translational Research

The costs and efforts for sample preparation of hundreds of individuals, their genomic enrichment for regions of interest, and sufficient deep sequencing bring a significant burden to next-generation sequencing-based experiments. We investigated whet

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abc2e38bd2b900549e2a301e3f211ae1
https://doi.org/10.1007/s12265-011-9263-5

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