Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Tanya Sukonnik"'
Autor:
James Ellis, Philippe Leboulch, Tanya Sukonnik, Bruno Dalle, Rob Pawliuk, Peter Pasceri, Ozan Alkan, Shuyuan Yao, Joel E. Rubin
Publikováno v:
Molecular Therapy. 11:591-599
beta-Globin transgenes regulated by the locus control region (LCR) are dominantly silenced by linked bacterial reporter genes in transgenic mice. Enhanced green fluorescent protein (eGFP) from jellyfish is an alternative reporter used in retrovirus v
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5e76fb94e24ea0cd2e5ac19a4ad9bcd
https://doi.org/10.1016/j.ymthe.2004.11.012
https://doi.org/10.1016/j.ymthe.2004.11.012
Publikováno v:
Molecular Therapy. 10:27-36
Retrovirus silencing in stem cells produces silent or variegated provirus. Additional memory and extinction mechanisms act during differentiation. Here we show that retrovirus is silent or variegated in mouse embryonic stem (ES) cells that are de nov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3232aac8459f19571e95f0c012111b7
https://doi.org/10.1016/j.ymthe.2004.04.007
https://doi.org/10.1016/j.ymthe.2004.04.007
Autor:
Tanya Sukonnik, Rikki R. Bharadwaj, James Ellis, Félix Recillas-Targa, Peter Pasceri, Adam G. West, Cameron S. Osborne, Dylan Pannell, Shuyuan Yao
Publikováno v:
Nucleic Acids Research. 31:5317-5323
Silencing of retrovirus vectors poses a significant obstacle to genetic manipulation of stem cells and their use in gene therapy. We describe a mammalian silencer blocking assay using insulator elements positioned between retrovirus silencer elements
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::564906d1cc5f9589887e851f19d1aa69
https://doi.org/10.1093/nar/gkg742
https://doi.org/10.1093/nar/gkg742
Retrovirus vector silencing is de novo methylase independent and marked by a repressive histone code
Autor:
Dylan Pannell, James Ellis, Angelo Karaiskakis, Shuyuan Yao, Howard D. Lipshitz, Peter Pasceri, Tanya Sukonnik, Cameron S. Osborne, Masaki Okano, En Li
Publikováno v:
The EMBO Journal. 19:5884-5894
Retrovirus vectors are de novo methylated and transcriptionally silent in mammalian stem cells. Here, we identify epigenetic modifications that mark retrovirus‐silenced transgenes. We show that murine stem cell virus (MSCV) and human immunodeficien
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e174edc8aa42a246e246bc5f0c817014
https://doi.org/10.1093/emboj/19.21.5884
https://doi.org/10.1093/emboj/19.21.5884
Publikováno v:
Molecular therapy : the journal of the American Society of Gene Therapy. 15(4)
Transgene variegation is caused by epigenetic switching between expressing and silent states. gamma-retrovirus vectors can be variegated in stem cells, but the dynamics of epigenetic remodeling during transgene variegation are unknown. Here, we measu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f803901a060a7e682e6abe90884805ae
https://pubmed.ncbi.nlm.nih.gov/17285140
https://pubmed.ncbi.nlm.nih.gov/17285140
Autor:
James Ellis, Peter Pasceri, Rakesh Singal, Cameron S. Osborne, Gordon D. Ginder, Tanya Sukonnik
Transcriptional silencing of retrovirus vectors in primitive stem cells is a major obstacle to current gene therapy approaches to the treatment of blood diseases (5, 50, 52). Due to the difficulty in purifying hematopoietic stem cells and infecting t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d391f22ee716bab174bc98207ce5f20d
https://europepmc.org/articles/PMC112606/
https://europepmc.org/articles/PMC112606/
Publikováno v:
Molecular Therapy. 9:S154-S155
We show here that retrovirus and lentivirus vectors are subject to silencing in mouse ES cells producing single-copy proviruses that are silent, variegated or low expressing at best. The mechanism of retrovirus silencing was studied by infecting wild
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e44fd7a4c1a5100b2c16dbeb6aaef0ed
https://doi.org/10.1016/j.ymthe.2004.06.354
https://doi.org/10.1016/j.ymthe.2004.06.354
Publikováno v:
Molecular Therapy. 11:S33
Top of pageAbstract Epigenetic programming is mitotically heritable and affects gene expression without altering DNA sequence. The transition from active to inactive gene expression is referred to as gene silencing. Transgenes are often subject to pa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::24054249d149c9cfbe14ce7f9934c26f
https://doi.org/10.1016/j.ymthe.2005.06.057
https://doi.org/10.1016/j.ymthe.2005.06.057