Phagemid-based capsid system for CRISPR-Cas13a antimicrobials targeting methicillin-resistant Staphylococcus aureus.

Autor: Li FY; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Tan XE; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Shimamori Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Kiga K; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan.; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan., Veeranarayanan S; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Watanabe S; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Nishikawa Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan.; EIKEN CHEMICAL CO.,LTD., Shimotsuga District, Tochigi, 329-0114, Japan., Aiba Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Sato'o Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Miyanaga K; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Sasahara T; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Hossain S; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Thitiananpakorn K; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Kawaguchi T; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Nguyen HM; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Yeo Syin Lian A; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Sultana S; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Alessa O; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Kumwenda G; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Sarangi J; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Revilleza JEC; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Baranwal P; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Faruk MO; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Hidaka Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Thu M; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Arbaah M; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Batbold A; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Maniruzzaman; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Liu Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Duyen HTM; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Sugano T; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Tergel N; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan., Shimojyo T; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan., Cui L; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke city, Tochigi, 329-0498, Japan. longzhu@jichi.ac.jp.

Publikováno v: Communications biology [Commun Biol] 2024 Sep 13; Vol. 7 (1), pp. 1129. Date of Electronic Publication: 2024 Sep 13.

Efficient synthesis of CRISPR-Cas13a-antimicrobial capsids against MRSA facilitated by silent mutation incorporation.

Autor: Shimamori Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Tan XE; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Li FY; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Nishikawa Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan.; EIKEN CHEMICAL CO., LTD., Nogi, Shimotsuga District, Tochigi, 329-0114, Japan., Watanabe S; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Sasahara T; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Miyanaga K; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Aiba Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Veeranarayanan S; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Thitiananpakorn K; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Nguyen HM; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Batbold A; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Nayanjin T; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Lian AYS; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Hossain S; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Kawaguchi T; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Alessa O; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Kumwenda G; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Sarangi J; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Revilleza JEC; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Baranwal P; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Arbaah M; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Maniruzzaman; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Yi L; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Duyen HTM; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Sugano T; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Sultana S; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Faruk MO; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Hidaka Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Thu M; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan., Shimojyo T; EIKEN CHEMICAL CO., LTD., Nogi, Shimotsuga District, Tochigi, 329-0114, Japan., Kiga K; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan.; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan., Cui L; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke City, Tochigi, 329-0498, Japan. longzhu@jichi.ac.jp.

Publikováno v: Scientific reports [Sci Rep] 2024 Jul 13; Vol. 14 (1), pp. 16225. Date of Electronic Publication: 2024 Jul 13.

The Association Between Onset of Staphylococcal Non-menstrual Toxic Shock Syndrome With Inducibility of Toxic Shock Syndrome Toxin-1 Production.

Autor: Taki Y; Division of Bacteriology, Jichi Medical School, Tochigi, Japan.; Department of Gastroenterological Surgery, Shizuoka General Hospital, Shizuoka, Japan., Watanabe S; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Sato'o Y; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Tan XE; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Ono HK; Department of Zoonoses, School of Veterinary Medicine, Kitasato University, Towada, Japan., Kiga K; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Aiba Y; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Sasahara T; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Azam AH; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Thitiananpakorn K; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Veeranarayanan S; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Li FY; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Zhang Y; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Kawaguchi T; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Hossain S; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Maniruzzaman; Division of Bacteriology, Jichi Medical School, Tochigi, Japan., Hu DL; Department of Zoonoses, School of Veterinary Medicine, Kitasato University, Towada, Japan., Cui L; Division of Bacteriology, Jichi Medical School, Tochigi, Japan.

Publikováno v: Frontiers in microbiology [Front Microbiol] 2022 Mar 14; Vol. 13, pp. 765317. Date of Electronic Publication: 2022 Mar 14 (Print Publication: 2022).

Identification and characterization of mutations responsible for the β-lactam resistance in oxacillin-susceptible mecA-positive Staphylococcus aureus.

Autor: Boonsiri T; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Watanabe S; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Tan XE; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Thitiananpakorn K; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Narimatsu R; School of Medicine, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Sasaki K; School of Medicine, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Takenouchi R; School of Medicine, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Sato'o Y; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Aiba Y; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Kiga K; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Sasahara T; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Taki Y; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Li FY; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Zhang Y; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Azam AH; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Kawaguchi T; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan., Cui L; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan. longzhu@jichi.ac.jp.

Publikováno v: Scientific reports [Sci Rep] 2020 Oct 09; Vol. 10 (1), pp. 16907. Date of Electronic Publication: 2020 Oct 09.

Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria.

Autor: Kiga K; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Tan XE; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Ibarra-Chávez R; Institute of Infection, Immunity & Inflammation, University of Glasgow, Glasgow, G12 8TA, UK., Watanabe S; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Aiba Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Sato'o Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Li FY; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Sasahara T; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Cui B; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Kawauchi M; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Boonsiri T; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Thitiananpakorn K; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Taki Y; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Azam AH; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan., Suzuki M; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Penadés JR; Institute of Infection, Immunity & Inflammation, University of Glasgow, Glasgow, G12 8TA, UK., Cui L; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Tochigi, Japan. longzhu@jichi.ac.jp.

Publikováno v: Nature communications [Nat Commun] 2020 Jun 10; Vol. 11 (1), pp. 2934. Date of Electronic Publication: 2020 Jun 10.