Zobrazeno 1 - 10
of 120
pro vyhledávání: '"Tan Inoue"'
Publikováno v:
PLoS ONE, Vol 10, Iss 3, p e0120576 (2015)
An affinity resin-based pull-down method is convenient for the purification of biochemical materials. However, its use is difficult for the isolation of a molecular complex fully loaded with multiple components from a reaction mixture containing the
Externí odkaz:
https://doaj.org/article/2a3d290257c8490e9e436d5234814eb2
Publikováno v:
Biochemical and Biophysical Research Communications. 466:388-392
RNA-protein complexes (RNPs) are useful for constructing functional nano-objects because a variety of functional proteins can be displayed on a designed RNA scaffold. Here, we report circular permutations of an RNA-binding protein L7Ae based on the t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8203593b949a81a70a92cdd388453c14
https://doi.org/10.1016/j.bbrc.2015.09.035
https://doi.org/10.1016/j.bbrc.2015.09.035
Publikováno v:
FEBS Letters. 589:2424-2428
Multifunctional molecular complexes are valuable tools with a variety of applications. We have developed an RNA–protein complex (RNP) containing three different proteins attached to the tips of a triangular RNA scaffold. We designed and constructed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75828389fe45d1dceff457cb55f09eab
https://doi.org/10.1016/j.febslet.2015.07.005
https://doi.org/10.1016/j.febslet.2015.07.005
Publikováno v:
Nucleic Acids Research
The three-dimensional (3D) structures of many biomacromolecules have been solved to reveal the functions of these molecules. However, these 3D structures have rarely been applied to constructing efficient molecular devices that function in living cel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0243ac999c9c65390d582b7153146603
http://europepmc.org/articles/PMC3467064
http://europepmc.org/articles/PMC3467064
Autor:
Hirohide Saito, Kei Endo, Karin Hayashi, Yoshihiko Fujita, James A. Stapleton, Masahiro Takinoue, Tan Inoue
Publikováno v:
ACS Synthetic Biology
Feedback regulation plays a crucial role in dynamic gene expression in nature, but synthetic translational feedback systems have yet to be demonstrated. Here we use an RNA/protein interaction-based synthetic translational switch to create a feedback
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0746f3b91fa726eb14e6dcba9eb90322
http://t2r2.star.titech.ac.jp/cgi-bin/publicationinfo.cgi?q_publication_content_number=CTT100724602
http://t2r2.star.titech.ac.jp/cgi-bin/publicationinfo.cgi?q_publication_content_number=CTT100724602
Publikováno v:
Biomacromolecules. 12:3945-3951
AzoTAB, a photosensitive azobenzene cationic surfactant, which phototriggers translation activity through light-regulated condensation of mRNA, is added to a translation solution containing several mRNAs, which can be selectively silenced by specific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b23d0e11b7be3fa4083b48ac88cf32e7
https://doi.org/10.1021/bm200962s
https://doi.org/10.1021/bm200962s
Publikováno v:
Journal of Molecular Evolution. 73:221-229
RNA can function both as an informational molecule and as a catalyst in living organisms. This duality is the premise of the RNA world hypothesis. However, one flaw in the hypothesis that RNA was the most essential molecule in primitive life is that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6814ed37a473113c82f4ba3fce202d9e
https://doi.org/10.1007/s00239-011-9471-2
https://doi.org/10.1007/s00239-011-9471-2
Publikováno v:
Archives of Biochemistry and Biophysics. 490:163-170
The DSL ribozyme is a class of artificial ligase ribozymes with a highly modular architecture, which catalyzes template-directed RNA ligation on a helical substrate module that can be either covalently connected (cis-DSL) or physically separated (tra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0999abdc2cf472691d62ae160788516d
https://doi.org/10.1016/j.abb.2009.08.020
https://doi.org/10.1016/j.abb.2009.08.020
Publikováno v:
Nucleic Acids Research
Naturally occurring proteins in cellular networks often share peptide motifs. These motifs have been known to play a pivotal role in protein interactions among the components of a network. However, it remains unknown how these motifs have contributed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0dff713bf37eb4b4a5befbf0e4fb0c6
https://doi.org/10.1093/nar/gkm692
https://doi.org/10.1093/nar/gkm692
Autor:
Hirohisa Ohno, Tan Inoue
Publikováno v:
ACS nano. 9(5)
RNA nanotechnology has been established by employing the molecular architecture of RNA structural motifs. Here, we report two designed RNA-protein complexes (RNPs) composed of ribosomal protein L1 (RPL1) and its RNA-binding motif that are square-shap
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77d3f5571b4fb118a0e10c94e72bfcfe
https://pubmed.ncbi.nlm.nih.gov/25933202
https://pubmed.ncbi.nlm.nih.gov/25933202