Fluorescence anisotropy assays for high throughput screening of compounds binding to lipid II, PBP1b, FtsW and MurJ

Autor: Adrien Boes, Samir Olatunji, Tamimount Mohammadi, Eefjan Breukink, Mohammed Terrak

Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)

Abstract Lipid II precursor and its processing by a flippase and peptidoglycan polymerases are considered key hot spot targets for antibiotics. We have developed a fluorescent anisotropy (FA) assay using a unique and versatile probe (fluorescent lipi

Externí odkaz: https://doaj.org/article/01e5e5b8ff56424899107e86065805e5

Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers.

Autor: Yao Liu, Elisabete C C M Moura, Jonas M Dörr, Stefan Scheidelaar, Michal Heger, Maarten R Egmond, J Antoinette Killian, Tamimount Mohammadi, Eefjan Breukink

Publikováno v: PLoS ONE, Vol 13, Iss 11, p e0206692 (2018)

As an integral membrane protein, purification and characterization of phospho-N- acetylmuramyl- pentapeptide translocase MraY have proven difficult. Low yield and concerns of retaining stability and activity after detergent solubilization have hamper

Externí odkaz: https://doaj.org/article/8c3f1fcc5d7a4a379f1551a30d646bc7

The essential peptidoglycan glycosyltransferase MurG forms a complex with proteins involved in lateral envelope growth as well as with proteins involved in cell division in Escherichia coli

Autor: Muriel Crouvoisier, Dominique Mengin-Lecreulx, Aneta Karczmarek, Ahmed Bouhss, Tamimount Mohammadi, Tanneke den Blaauwen

Publikováno v: Molecular Microbiology
Molecular Microbiology, 65(4), 1106-1121. Wiley-Blackwell

In Escherichia coli many enzymes including MurG are directly involved in the synthesis and assembly of peptidoglycan. MurG is an essential glycosyltransferase catalysing the last intracellular step of peptidoglycan synthesis. To elucidate its role du

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bb01b9cf6aaf7a2ed5e65554ee1bcca
https://dare.uva.nl/personal/pure/en/publications/the-essential-peptidoglycan-glycosyltransferase-murg-forms-a-complex-with-proteins-involved-in-lateral-envelope-growth-as-well-as-with-proteins-involved-in-cell-division-in-escherichia-coli(70a32b51-c964-418f-890a-338c6d90b76f).html

Removal of contaminating DNA from commercial nucleic acid extraction kit reagents

Autor: Christina M. J. E. Vandenbroucke-Grauls, Tamimount Mohammadi, Paul H. M. Savelkoul, Henk W. Reesink

Publikováno v: Journal of microbiological methods, 61(2), 285-288. Elsevier
Mohammadi, T, Reesink, H W, Vandenbroucke-Grauls, C M J E & Savelkoul, P H M 2005, ' Removal of contaminating DNA from commercial nucleic acid extraction kit reagents ', Journal of Microbiological Methods, vol. 61, no. 2, pp. 285-288 . https://doi.org/10.1016/j.mimet.2004.11.018
Journal of Microbiological Methods, 61(2), 285-288. Elsevier

Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d53b3d0cf0a1f8a2e94009ece2517c4
https://doi.org/10.1016/j.mimet.2004.11.018

Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membrane

Autor: Tamimount, Mohammadi, Vincent, van Dam, Robert, Sijbrandi, Thierry, Vernet, André, Zapun, Ahmed, Bouhss, Marlies, Diepeveen-de Bruin, Martine, Nguyen-Distèche, Ben, de Kruijff, Eefjan, Breukink

Publikováno v: The EMBO journal. 30(8)

Bacterial cell growth necessitates synthesis of peptidoglycan. Assembly of this major constituent of the bacterial cell wall is a multistep process starting in the cytoplasm and ending in the exterior cell surface. The intracellular part of the pathw

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2c2162ca952c48b1558934a20cd5fab1
https://pubmed.ncbi.nlm.nih.gov/21494274