Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Tamara Miljuš"'
Publikováno v:
Bio-Protocol, Vol 10, Iss 1 (2020)
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants
Externí odkaz:
https://doaj.org/article/f8fc9d549834442c91e594c150503448
Autor:
Franziska M. Heydenreich, Tamara Miljuš, Rolf Jaussi, Roger Benoit, Dalibor Milić, Dmitry B. Veprintsev
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Abstract Site-directed scanning mutagenesis is a powerful protein engineering technique which allows studies of protein functionality at single amino acid resolution and design of stabilized proteins for structural and biophysical work. However, crea
Externí odkaz:
https://doaj.org/article/1b11ce312b1949debc1d11e568ec22af
Autor:
Thais Gazzi, Benjamin Brennecke, Kenneth Atz, Claudia Korn, David Sykes, Gabriel Forn-Cuni, Patrick Pfaff, Roman C. Sarott, Matthias V. Westphal, Yelena Mostinski, Leonard Mach, Malgorzata Wasinska-Kalwa, Marie Weise, Bradley L. Hoare, Tamara Miljuš, Maira Mexi, Nicolas Roth, Eline J. Koers, Wolfgang Guba, André Alker, Arne C. Rufer, Eric A. Kusznir, Sylwia Huber, Catarina Raposo, Elisabeth A. Zirwes, Anja Osterwald, Anto Pavlovic, Svenja Moes, Jennifer Beck, Matthias Nettekoven, Irene Benito-Cuesta, Teresa Grande, Faye Drawnel, Gabriella Widmer, Daniela Holzer, Tom van der Wel, Harpreet Mandhair, Michael Honer, Jürgen Fingerle, Jörg Scheffel, Johannes Broichhagen, Klaus Gawrisch, Julián Romero, Cecilia J. Hillard, Zoltan V. Varga, Mario van der Stelt, Pal Pacher, Jürg Gertsch, Christoph Ullmer, Peter J. McCormick, Sergio Oddi, Herman P. Spaink, Mauro Maccarrone, Dmitry B. Veprintsev, Erick M. Carreira, Uwe Grether, Marc Nazaré
Publikováno v:
Gazzi, Thais; Brennecke, Benjamin; Atz, Kenneth; Korn, Claudia; Sykes, David; Forn-Cuni, Gabriel; Pfaff, Patrick; Sarott, Roman C; Westphal, Matthias V; Mostinski, Yelena; Mach, Leonard; Wasinska-Kalwa, Malgorzata; Weise, Marie; Hoare, Bradley L; Miljuš, Tamara; Mexi, Maira; Roth, Nicolas; Koers, Eline J; Guba, Wolfgang; Alker, André; ... (2022). Detection of cannabinoid receptor type 2 in native cells and zebrafish with a highly potent, cell-permeable fluorescent probe. Chemical Science, 13(19), pp. 5539-5545. The Royal Society of Chemistry 10.1039/d1sc06659e
Chemical Science, 13(19), 5539-5545
Chemical Science
Chemical Science, 13(19), 5539-5545
Chemical Science
Despite its essential role in the (patho)physiology of several diseases, CB2R tissue expression profiles and signaling mechanisms are not yet fully understood. We report the development of a highly potent, fluorescent CB2R agonist probe employing str
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69e61ac61b6e5b29da1ceeb545f2c056
http://hdl.handle.net/11697/192023
http://hdl.handle.net/11697/192023
Publikováno v:
Physiological reviews. 101(3)
G protein-coupled receptors (GPCRs) regulate many cellular and physiological processes, responding to a diverse range of extracellular stimuli including hormones, neurotransmitters, odorants, and light. Decades of biochemical and pharmacological stud
Autor:
Mark Rogers-Evans, Matthias Nettekoven, Michel Bouvier, Uwe Grether, Franziska M. Heydenreich, Thais Gazzi, Anja Osterwald, Marc Nazaré, Atsushi Kimbara, Tamara Miljuš, Christoph Ullmer, Christian Le Gouill, Dmitry B. Veprintsev, Arne C. Rufer, Elisabeth A. Zirwes, Wolfgang Guba, Jürgen Fingerle
Cannabinoid CB1 and CB2 receptors are members of the G protein-coupled receptor family, which is the largest class of membrane proteins in the human genome. As part of the endocannabinoid system, they have many regulatory functions in the human body.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a5b2aa7bcf5fc2433f62078972394e5
https://doi.org/10.1101/2020.11.09.375162
https://doi.org/10.1101/2020.11.09.375162
Sensitive protein stability assays for membrane proteins are crucial for developing purification protocols, for structural and biophysical characterisation and drug discovery. Here, we describe a novel high-throughput 384-well FRET-based thermostabil
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d5c40c19803545d4e4a557244691a161
https://doi.org/10.1101/2020.07.07.191957
https://doi.org/10.1101/2020.07.07.191957
Publikováno v:
Bio Protoc
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc3239a6525c420fbeeacf6e10e81b37
https://europepmc.org/articles/PMC7842796/
https://europepmc.org/articles/PMC7842796/
Autor:
Natasha L. Grimsey, Dmitry B. Veprintsev, Roman C. Sarott, Teresa Grande, Patrick Pfaff, Jürg Gertsch, Pal Pacher, Erick M. Carreira, Christoph Ullmer, Hermon Asmelash, Pattarin Hompluem, Svenja Moes, Daniela Holzer, Catarina Raposo, William R. Drobyski, Sylwia Huber, Jennifer Beck, Uwe Grether, Marc Nazaré, Claudia Korn, Zoltán Varga, Eline J. Koers, Jacopo Piovesan, Irene Benito-Cuesta, Klaus Gawrisch, Matthias Westphal, Benjamin Brennecke, Gabriella Widmer, Yurii Saroz, Eric Kusznir, Tom van der Wel, Anto Pavlovic, Kenneth Atz, Man C Vong, Tamara Miljuš, Peter J. McCormick, Mauro Maccarrone, Harpreet Mandhair, Samuel Ruiz de Martín Esteban, Sergio Oddi, Yelena Mostinski, Julián Romero, Elisabeth A. Zirwes, Anja Osterwald, Jürgen Fingerle, Cecilia J. Hillard, David A. Sykes, Alexei Yeliseev, Faye M. Drawnel, Mario van der Stelt, Marie Weise, Arne C. Rufer, Cheng-Yin Yuan, Thais Gazzi, Nicolas J. Roth, Michael Honer, Wolfgang Guba
Publikováno v:
Journal of the American Chemical Society
Journal of the American Chemical Society, 142(40), 16953-16964
Journal of the American Chemical Society, 142(40), 16953-16964
Pharmacological modulation of cannabinoid type 2 receptor (CB2R) holds promise for the treatment of numerous conditions, including inflammatory diseases, autoimmune disorders, pain, and cancer. Despite the significance of this receptor, researchers l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1774cb215365081fabff5fd4b151b22f
http://hdl.handle.net/11697/158602
http://hdl.handle.net/11697/158602
Publikováno v:
Methods in Molecular Biology ISBN: 9781071603727
G protein-coupled receptors (GPCRs) are versatile membrane proteins involved in the regulation of many physiological processes and pathological conditions, making them interesting pharmacological targets. In order to study their structure and functio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::09483e4c2c1c0585447632aceced4675
https://doi.org/10.1007/978-1-0716-0373-4_8
https://doi.org/10.1007/978-1-0716-0373-4_8
Autor:
Faye M. Drawnel, Jürgen Fingerle, Cecilia J. Hillard, David A. Sykes, Erick M. Carreira, Yurii Saroz, Christoph Ullmer, Natasha L. Grimsey, Daniela Holzer, Maira Mexi, Anja Osterwald, Dmitry B. Veprintsev, Patrick Pfaff, André Anker, Irene Benito-Cuesta, Matthias Westphal, Klaus Gawrisch, Benjamin Brennecke, Marie Weise, Julián Romero, Jennifer Beck, Anto Pavlovic, Gabriella Widmer, Kenneth Atz, Peter J. McCormick, Harpreet Mandhair, Michael Honer, Uwe Grether, Wolfgang Guba, Pal Pacher, Svenja Moes, Sylwia Huber, Bradley L. Hoare, Arne C. Rufer, Yelena Mostinski, Mario van der Stelt, Jürg Gertsch, Mauro Maccarrone, Claudia Korn, Roman C. Sarott, Elisabeth A. Zirwes, Catarina Raposo, Zoltán Varga, Tamara Miljuš, Thais Gazzi, Tom van der Wel, Teresa Grande, Eric Kusznir, Sergio Oddi, Marc Nazaré
Cannabinoid type 2 receptor (CB2R) is a fundamental part of the endocannabinoid signaling system (eCB system), and is known to play an important role in tissue injury, inflammation, cancer and pain. In stark contrast to its significance, the underlyi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e3cac06b852c3e7933e52745f95a639
https://doi.org/10.26434/chemrxiv.10283027.v1
https://doi.org/10.26434/chemrxiv.10283027.v1