Zobrazeno 1 - 10
of 66
pro vyhledávání: '"Takuya Nishigaki"'
Autor:
Julio C. Chávez, Gabriela Carrasquel-Martínez, Sandra Hernández-Garduño, Arturo Matamoros Volante, Claudia L. Treviño, Takuya Nishigaki, Alberto Darszon
Publikováno v:
Cells, Vol 13, Iss 10, p 865 (2024)
As in most cells, intracellular pH regulation is fundamental for sperm physiology. Key sperm functions like swimming, maturation, and a unique exocytotic process, the acrosome reaction, necessary for gamete fusion, are deeply influenced by pH. Sperm
Externí odkaz:
https://doaj.org/article/e52fa18d2dd749298c48152739ed3527
Autor:
Francisco Romero, Takuya Nishigaki
Publikováno v:
Zoological Letters, Vol 5, Iss 1, Pp 1-12 (2019)
Abstract Background CatSper is a sperm-specific calcium ion (Ca2+) channel, which regulates sperm flagellar beating by tuning cytoplasmic Ca2+ concentrations. Although this Ca2+ channel is essential for mammalian fertilization, recent bioinformatics
Externí odkaz:
https://doaj.org/article/6a39945beb4b446e8a8dde42c160d987
Autor:
Daniel A Priego-Espinosa, Alberto Darszon, Adán Guerrero, Ana Laura González-Cota, Takuya Nishigaki, Gustavo Martínez-Mekler, Jorge Carneiro
Publikováno v:
PLoS Computational Biology, Vol 16, Iss 3, p e1007605 (2020)
Intracellular calcium ([Ca2+]i) is a basic and ubiquitous cellular signal controlling a wide variety of biological processes. A remarkable example is the steering of sea urchin spermatozoa towards the conspecific egg by a spatially and temporally orc
Externí odkaz:
https://doaj.org/article/3a4225ee9dc14cdc9897a77c2c9f34dc
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 7 (2020)
Intracellular pH (pHi) plays a crucial role in mammalian sperm physiology. However, it is a challenging task to acquire quantitative single sperm pHi images due to their small size and beating flagella. In this study, we established a robust pHi imag
Externí odkaz:
https://doaj.org/article/0032df5d0f24414da3fa8b7c53a4d032
Publikováno v:
BioTechniques, Vol 65, Iss 4, Pp 211-218 (2018)
Fluorescence (or Förster) resonance energy transfer (FRET) is a straightforward and sensitive technique to evaluate molecular interactions. However, most of the popular FRET pairs suffer cross-excitation of the acceptor, which could lead to false po
Externí odkaz:
https://doaj.org/article/6b7da8b72f064114a9729b4828ab93cd
Publikováno v:
BioTechniques, Vol 41, Iss 2, Pp 191-197 (2006)
Excited fluorophores produce reactive oxygen species that are toxic toward many live cells (phototoxicity) and accelerate bleaching of the fluorophores during the course of extended or repeated measurements (photobleaching). We recently developed an
Externí odkaz:
https://doaj.org/article/e896a17712c844aabbcbac14fcd81f3b
Autor:
Sandra Hernández-Garduño, Julio C Chávez, Arturo Matamoros-Volante, Yoloxochitl Sánchez-Guevara, Paulina Torres, Claudia L Treviño, Takuya Nishigaki
Publikováno v:
Reproduction (Cambridge, England). 164(4)
In brief Hyperpolarization of the membrane potential is a crucial step for mammalian sperm maturation. This work demonstrates that this membrane potential change likely activates a sperm-specific sodium/proton exchanger to induce alkalization in mous
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fbf6947900585d119e015fe7aa2b15e
https://pubmed.ncbi.nlm.nih.gov/35900329
https://pubmed.ncbi.nlm.nih.gov/35900329
Autor:
César Arcos-Hernández, Esteban Suárez-Delgado, León D. Islas, Francisco Romero, Ignacio López-González, Hui-wang Ai, Takuya Nishigaki
Publikováno v:
Protein Expression and Purification. 201:106172
Heterologous expression systems have been used as a powerful experimental strategy to study the function of many proteins, particularly ion transporters. For this experiment, it is fundamental to prepare an expression vector encoding a protein of int
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b561b05344f81844c2ec4d3ff0267d7
https://doi.org/10.1016/j.pep.2022.106172
https://doi.org/10.1016/j.pep.2022.106172
Autor:
Hernández-Garduño, Sandra, Chávez, Julio C., Matamoros-Volante, Arturo, Sánchez-Guevara, Yoloxochitl, Torres, Paulina, Treviño, Claudia L., Takuya Nishigaki
Publikováno v:
Reproduction; Oct2022, Vol. 164 Issue 4, p125-134, 10p
Autor:
Maricela García-Castañeda, Guillermo Avila, Teresa Mancilla-Percino, Juan A.M. De La Rosa, Takuya Nishigaki, Juan Carlos Gomora
Publikováno v:
Molecular pharmacology. 98(3)
Amino acid-derived isoindolines are synthetic compounds that were created with the idea of investigating their biological actions. The amino acid moiety was included on the grounds that it may help to avoid toxic effects. Recently, the isoindoline MD
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4cacbdb834b3247cd672df93d37714e
https://pubmed.ncbi.nlm.nih.gov/32587097
https://pubmed.ncbi.nlm.nih.gov/32587097