Výsledky vyhledávání - "Taku Arakawa"

Gene expression analysis in colorectal cancer using practical DNA array filter

Autor: Norihiro Nishimura, Mikio Shiomi, Taku Arakawa, Kiyotaka Okuno, Kenji Minami, Masayuki Yasutomi, Kazuki Ueda, Jin-ichi Hida

Publikováno v: Diseases of the Colon & Rectum. 44:295-299

PURPOSE: We examined the usability of a newly developed, compact-sized DNA array filter for studying the gene expression pattern of individual colorectal cancer. METHODS: Complementary DNA probes were prepared from mRNA extracted from colonic cancer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6afc6a463e1c29d8ea6b1bce637560b
https://doi.org/10.1007/bf02234309

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Construction and characterization of N-terminally truncated DNA polymerase from Thermus thermophilus

Autor: Hideyuki Komatsubara, Keiko Tanaka, Masanori Oka, Yasufumi Shima, Bunsei Kawakami, Taku Arakawa, Shigenori Emi, Hiroaki Inoue, Seiji Negoro, Itaru Urabe, Akihisa Hasegawa, Katsunori Ikeda, Tetsuya Yomo

Publikováno v: Journal of Fermentation and Bioengineering. 81:504-510

Various plasmids harboring the truncated DNA polymerase gene ( polA ) from Thermus thermophilus HB8 ( Tth polymerase) were constructed. The most thermostable Tth Δ NF 2 polymerase [the gene product of polA Δ NF 2, which lacked a 751-bp region (regi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c688d2cbfb4ee98baaa50ab2c006abf3
https://doi.org/10.1016/0922-338x(96)81470-x

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A Novel Method for Generating Nested Deletions Using the in Vitro Bacteriophage T3 DNA Packaging System

Autor: Osamu Ohara, Masanori Oka, Miyo Terao Morita, Hisao Fujisawa, Ayako Tanaka, Taku Arakawa, Hisako Kato, Yutaka Kawarabayasi

Publikováno v: DNA Research. 1:289-296

To sequence a DNA segment inserted into a cosmid vector under the directed sequencing strategy, we established a simple and rapid method for generating nested deletions which uses the in vitro packaging system of bacteriophage T3 DNA. The principle i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60d41261528c2397a9ee570031592e86
https://doi.org/10.1093/dnares/1.6.289

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Reductive and nucleophilic activation products of dynemicin A with methyl thioglycolate. A rational mechanism for DNA cleavage of the thiol-activated dynemicin A

Autor: Yukio Sugiura, Masataka Konishi, Taku Arakawa, Hiroaki Ohkuma, Takashi Shiraki, Motonari Uesugi

Publikováno v: Biochemistry. 30:2989-2992

The reaction products of methyl thioglycolate with dynemicin A, dynemicin H and dynemicin S, were isolated by HPLC purification and identified spectroscopically. The major product, dynemicin H (C30H23NO9), was determined to be a C-8 hydrogen analogue

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7c54a7c3f4036c0f977169338838909
https://doi.org/10.1021/bi00226a001

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Application of N-terminally truncated DNA polymerase from Thermus thermophilus (delta Tth polymerase) to DNA sequencing and polymerase chain reactions: comparative study of delta Tth and wild-type Tth polymerases

Autor: Itaru Urabe, Hideyuki Komatsubara, Yusaku Tatsumi, Seiji Negoro, Hiroaki Inoue, Bunsei Kawakami, Boonsri Jongsareejit, Taku Arakawa, Masanori Oka, Yasufumi Shima, Katsunori Ikeda, Tetsuya Yomo, Shigenori Emi, Keiko Tanaka

Publikováno v: DNA research : an international journal for rapid publication of reports on genes and genomes. 3(2)

N-Terminally truncated DNA polymerase from Thermus thermophilus (delta Tth polymerase) lacking 5'-3' exonuclease activity was used for DNA sequencing and polymerase chain reaction (PCR). In contrast to the high background of the sequencing ladder obs

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9752092ad9b184ce7b94a5c5caa8692
https://pubmed.ncbi.nlm.nih.gov/8804860

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