Zobrazeno 1 - 10
of 245
pro vyhledávání: '"Takehiko Ogawa"'
Autor:
Hideaki Nakagiri, Takehiko Ogawa, Naohiro Ikeda, Shimpei Terasaka, Yuko Nukada, Masaaki Miyazawa
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-15 (2024)
Abstract Recently, it was reported that a testicular organ culture system (TOCS) using polydimethylsiloxane (PDMS) chips with excellent oxygen permeability and biocompatibility, called the PDMS-chip ceiling (PC) method, enables improved spermatogenes
Externí odkaz:
https://doaj.org/article/4da99f6c044f4c5b919224fa23c0662e
Autor:
Takehiko OGAWA, Takafumi MATSUMURA, Tatsuma YAO, Hiroshi KIMURA, Kiyoshi HASHIMOTO, Yu ISHIKAWA-YAMAUCHI, Takuya SATO
Publikováno v:
The Journal of Reproduction and Development, Vol 70, Iss 1, Pp 1-9 (2023)
Incorporation of bovine serum-derived albumin formulation (AlbuMAX) into a basic culture medium, MEMα, enables the completion of in vitro spermatogenesis through testicular tissue culture in mice. However, this medium was not effective in other anim
Externí odkaz:
https://doaj.org/article/3934235b85474ecd9faf744d9bf2c0a3
Autor:
Kiyoshi Hashimoto, Hisakazu Odaka, Yu Ishikawa-Yamauchi, Shino Nagata, Hiroko Nakamura, Hiroshi Kimura, Takuya Sato, Kazuhide Makiyama, Takehiko Ogawa
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)
Abstract The classical organ culture method, in which tissue is placed at the gas‒liquid interphase, is effective at inducing mouse spermatogenesis. However, due to reginal variations in the supply of oxygen and nutrients within a tissue, the progr
Externí odkaz:
https://doaj.org/article/c00ed94f705a4ea883a1835418e61435
Autor:
Takafumi Matsumura, Kumiko Katagiri, Tatsuma Yao, Yu Ishikawa-Yamauchi, Shino Nagata, Kiyoshi Hashimoto, Takuya Sato, Hiroshi Kimura, Takashi Shinohara, Makoto Sanbo, Masumi Hirabayashi, Takehiko Ogawa
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)
Abstract An in vitro spermatogenesis method using mouse testicular tissue to produce fertile sperm was established more than a decade ago. Although this culture method has generally not been effective in other animal species, we recently succeeded in
Externí odkaz:
https://doaj.org/article/840c25e053eb49ef8f12a549efe24686
Autor:
Kodai Hirano, Yuta Nonami, Yoshiaki Nakamura, Toshiyuki Sato, Takuya Sato, Kei-ichiro Ishiguro, Takehiko Ogawa, Shosei Yoshida
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-16 (2022)
High temperatures lead to DNA double-strand break accumulation and asynapsis that trigger the meiotic checkpoint and apoptosis of spermatocytes in ex vivo cultures, providing a rationale for the heat sensitivity of sperm production in mammals.
Externí odkaz:
https://doaj.org/article/ed394f52d2104958a154c11d3fdc3dc3
Autor:
Xuemin Feng, Takafumi Matsumura, Yuki Yamashita, Takuya Sato, Kiyoshi Hashimoto, Hisakazu Odaka, Yoshinori Makino, Yuki Okada, Hiroko Nakamura, Hiroshi Kimura, Teruo Fujii, Takehiko Ogawa
Publikováno v:
PLoS ONE, Vol 18, Iss 4, p e0283773 (2023)
Mouse spermatogenesis, from spermatogonial stem cell proliferation to sperm formation, can be reproduced in vitro by culturing testis tissue masses of neonatal mice. However, it remains to be determined whether this method is also applicable when tes
Externí odkaz:
https://doaj.org/article/dcc75a52154e4a718a7ad83316e22ce4
Autor:
Takafumi Matsumura, Takuya Sato, Takeru Abe, Hiroyuki Sanjo, Kumiko Katagiri, Hiroshi Kimura, Teruo Fujii, Hiromitsu Tanaka, Masumi Hirabayashi, Takehiko Ogawa
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract In vitro spermatogenesis (IVS) using air–liquid interphase organ culture method is possible with mouse testis tissues. The same method, however, has been hardly applicable to animals other than mice, only producing no or limited progressio
Externí odkaz:
https://doaj.org/article/92ad44ad0b874881b0c56413d201536a
Autor:
Hisanori Fukunaga, Kiichi Kaminaga, Takuya Sato, Karl T. Butterworth, Ritsuko Watanabe, Noriko Usami, Takehiko Ogawa, Akinari Yokoya, Kevin M. Prise
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
Abstract Microbeam radiotherapy (MRT) is based on a spatial fractionation of synchrotron X-ray microbeams at the microscale level. Although the tissue-sparing effect (TSE) in response to non-uniform radiation fields was recognized more than one centu
Externí odkaz:
https://doaj.org/article/4359fc8125a84c17ae1d481fc0cd10d4
Autor:
Mitsuru Komeya, Hiroyuki Yamanaka, Hiroyuki Sanjo, Masahiro Yao, Hiroko Nakamura, Hiroshi Kimura, Teruo Fujii, Takuya Sato, Takehiko Ogawa
Publikováno v:
Reproductive Medicine and Biology, Vol 18, Iss 4, Pp 362-369 (2019)
Abstract Purpose Mouse in vitro spermatogenesis is possible with classical organ culture methods, by placing the testis tissue at the interphase between culture medium and air. In this condition, however, a tissue piece tends to round up to be compac
Externí odkaz:
https://doaj.org/article/316480873e3045f5b49ab7f686b0864a
Time-course microarray transcriptome data of in vitro cultured testes and age-matched in vivo testes
Publikováno v:
Data in Brief, Vol 33, Iss , Pp 106482- (2020)
In vitro spermatogenesis, which produces fertile spermatozoa, has been successfully performed using an organ culture method from murine tissue. Here, we provide a dataset of time-course microarray transcriptome data of in vitro cultured neonate murin
Externí odkaz:
https://doaj.org/article/917cf30c4fe040b5b668b1fadba56152