Zobrazeno 1 - 10
of 400
pro vyhledávání: '"Takeharu Nagai"'
Autor:
Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki, Shota Yamashita, Arata Nagai, Kohki Okabe, Fan Li, Fei Yin, Keita Tominaga, Omer Faruk Bicer, Ryohei Noma, Bahareh Kiani, Olga Efa, Martin Büscher, Tetsuichi Wazawa, Masahiro Sonoshita, Hirofumi Shintaku, Takeharu Nagai, Sigurd Braun, Jessica P. Houston, Sherif Rashad, Kuniyasu Niizuma, Keisuke Goda
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract Flow cytometry is a vital tool in biomedical research and laboratory medicine. However, its accuracy is often compromised by undesired fluctuations in fluorescence intensity. While fluorescence lifetime imaging microscopy (FLIM) bypasses thi
Externí odkaz:
https://doaj.org/article/9e141a3a3e5140768e9b58bd2c11814e
Autor:
Hisashi Shidara, Taku Shirai, Ryohei Ozaki-Noma, Susumu Jitsuki, Takeharu Nagai, Kiwamu Takemoto
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-11 (2024)
Abstract Photosensitizing fluorescence protein is a promising tool for chromophore-assisted light inactivation (CALI) that enables specific oxidation and inactivation of intracellular molecules. However, a commonly used monomeric photosensitizing flu
Externí odkaz:
https://doaj.org/article/d9812696471b4205aa7c25cd9515a10f
Autor:
Yusuke Hara, Aya Ichiraku, Tomoki Matsuda, Ayuko Sakane, Takuya Sasaki, Takeharu Nagai, Kazuki Horikawa
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-10 (2024)
Abstract Genetically encoded Ca2+ indicators (GECIs) are versatile for live imaging of cellular activities. Besides the brightness and dynamic range of signal change of GECIs, Ca2+ affinity is another critical parameter for successful Ca2+ imaging, a
Externí odkaz:
https://doaj.org/article/259c0f687ec14fd98ae82919e8daab9f
Autor:
Kai Lu, Tetsuichi Wazawa, Tomoki Matsuda, Daria M. Shcherbakova, Vladislav V. Verkhusha, Takeharu Nagai
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-8 (2024)
Abstract Bacterial phytochromes are attractive molecular templates for engineering fluorescent proteins (FPs) because their near-infrared (NIR) emission significantly extends the spectral coverage of GFP-like FPs. Existing phytochrome-based FPs coval
Externí odkaz:
https://doaj.org/article/6e0be37afb3a45bc93e706a26513c174
Autor:
Hiroko Bannai, Akihiko Takashima, Yoshiyuki Soeda, Hideaki Yoshimura, Gen Matsumoto, Naruhiko Sahara, Michio Hiroshima, Mitsuru Hattori, Takeharu Nagai
Publikováno v:
Biophysics and Physicobiology, Vol 21 (2024)
Externí odkaz:
https://doaj.org/article/35e10b05dbb441129c568e7a67d24e95
Autor:
Takeharu Nagai
Publikováno v:
Biophysics and Physicobiology, Vol 21 (2024)
Externí odkaz:
https://doaj.org/article/f71e607d5d194c9bab2b67ae0b26513e
Autor:
Taro Ichimura, Taishi Kakizuka, Yuki Sato, Yoichiro Fujioka, Yusuke Ohba, Kazuki Horikawa, Takeharu Nagai
Publikováno v:
Biophysics and Physicobiology, Vol 21 (2024)
Singularity biology is a scientific field that targets drastic state changes in multicellular systems, aiming to discover the key cells that induce the state change and investigate the mechanisms behind them. To achieve this goal, we developed a tran
Externí odkaz:
https://doaj.org/article/390c720ed3fe49e0a9819cd39301dc90
Publikováno v:
STAR Protocols, Vol 5, Iss 1, Pp 102844- (2024)
Summary: cIPAD is a fluorescent indicator that allows the visualization of trans-interactions of clustered protocadherin (Pcdh), a cell adhesion molecule that mediates neuronal self-recognition. We describe steps for using HEK293T cells to visualize
Externí odkaz:
https://doaj.org/article/cd10787b880842a588f9cb5739db660e
Autor:
Masakazu Agetsuma, Issei Sato, Yasuhiro R. Tanaka, Luis Carrillo-Reid, Atsushi Kasai, Atsushi Noritake, Yoshiyuki Arai, Miki Yoshitomo, Takashi Inagaki, Hiroshi Yukawa, Hitoshi Hashimoto, Junichi Nabekura, Takeharu Nagai
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-22 (2023)
Abstract Associative learning is crucial for adapting to environmental changes. Interactions among neuronal populations involving the dorso-medial prefrontal cortex (dmPFC) are proposed to regulate associative learning, but how these neuronal populat
Externí odkaz:
https://doaj.org/article/545692dfb8104c3fa738efd0053e7876
Autor:
Mügen Terzioglu, Kristo Veeroja, Toni Montonen, Teemu O Ihalainen, Tiina S Salminen, Paule Bénit, Pierre Rustin, Young-Tae Chang, Takeharu Nagai, Howard T Jacobs
Publikováno v:
eLife, Vol 12 (2023)
Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric fluorescent temperature indicator targeted to mitochondria, the temperature of active mitochondria in four mammalian and one ins
Externí odkaz:
https://doaj.org/article/582ec16ea6924628942e3d6071e8abc5