Zobrazeno 1 - 10
of 155
pro vyhledávání: '"Takashi Murachi"'
Autor:
Takashi Murachi
Publikováno v:
Multiphase Biomedical Materials ISBN: 9780429087592
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ca58e7ca1eafcb154d92311d20aed453
https://doi.org/10.1201/9780429087592-9
https://doi.org/10.1201/9780429087592-9
Publikováno v:
Journal of Chromatography A. 597:435-442
Two or three different kinds of immobilized enzymes can be aligned in a minireactor so that sequential enzymatic reactions are carried out from upstream to downstream during flow-injection analysis. A lactate oxidase-catalase reactor, used as a preco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08a8374f137eeb0aedb0a7546ebe78d6
https://doi.org/10.1016/0021-9673(92)80142-h
https://doi.org/10.1016/0021-9673(92)80142-h
Autor:
Takashi Murachi, H. Keino, Shigeo Kashiwamata, S. Aono, Naoki Mizutani, Masayuki Totani, C. Hayakawa, Kimi Watanabe, Masaki Ikemoto
Publikováno v:
European Journal of Pediatrics. 150:800-803
In Japan, hyperargininemia has been reported in only 5 unrelated families and four patients are alive at present. In this study we examined arginase protein in erythrocytes of these Japanese patients using two analytical methods of immunoblotting and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61577d3c5b5e3972d3777a5f5afb9303
https://doi.org/10.1007/bf02026716
https://doi.org/10.1007/bf02026716
Autor:
Takashi Hamamoto, Kiichi Suzuki, Takao Yamamuro, Yasuaki Nakagawa, Takashi Murachi, Katsuji Shimizu, Takao Hamakubo, Woon Joo Lee
Publikováno v:
Journal of Orthopaedic Research. 9:26-36
Calpain is a Ca2(+)-dependent cysteine proteinase that has neutral pH optima. There are two classes of calpains that differ in their optimal calcium ion concentration for enzymatic activity. Calpain I requires a low concentration of Ca2+ for activati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b35ace7e5f96cc942f1bafe00f38615c
https://doi.org/10.1002/jor.1100090105
https://doi.org/10.1002/jor.1100090105
Publikováno v:
Biomedical Chromatography. 4:123-127
Chemiluminometric methods are described for the automated flow injection analysis of NADPH and NADH using an immobilized enzyme column reactor and serum magnesium. This application is for the clinical analysis of NADPH and NADH. The reactor for NADPH
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75f06412c686d6dd8a49fd8b4c9a32f2
https://doi.org/10.1002/bmc.1130040310
https://doi.org/10.1002/bmc.1130040310
Publikováno v:
Journal of Biotechnology. 14:33-41
A highly sensitive FIA system for chemiluminometric determination of reduced coenzyme, NADH, was developed, using immobilized NADH oxidase from Brevibacterium ammoniagenes . The enzyme catalyzed the oxidation of NADH generating hydrogen peroxide whic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f65d7ba7aa8af20bd4e9beeb947e8559
https://doi.org/10.1016/0168-1656(90)90015-4
https://doi.org/10.1016/0168-1656(90)90015-4
Publikováno v:
The Journal of Biochemistry. 107:440-444
Multicatalytic proteinase (MCP) was solubilized from human erythrocyte membrane with 0.1% Triton X-100 and purified to homogeneity using a combination of DEAE-cellulose, hydroxylapatite, and Ultrogel AcA34 chromatographies. This membranous MCP had si
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e88ca27375701c9e57a7c132f6d5a1fb
https://doi.org/10.1093/oxfordjournals.jbchem.a123064
https://doi.org/10.1093/oxfordjournals.jbchem.a123064
Autor:
Hiroyuki Hanzawa, Takashi Murachi, Masakazu Hatanaka, Kiyozo Asada, Yoji Arata, Fusao Kimizuka, Tadao Asano, Masatoshi Maki, Ikunoshin Kato, Takashi Uemori, Tomoko Shimojo
Publikováno v:
Biochemical and Biophysical Research Communications. 166:1485-1493
Expression plasmids were constructed from the cDNA of human calpastatin to examine the contribution to the inhibition of calpain of highly conserved sequences in each of four repetitive domains. A series of deletion derivatives of domain 1 proteins,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7620f53be485fb06cfbbcfca176f88ec
https://doi.org/10.1016/0006-291x(90)91035-q
https://doi.org/10.1016/0006-291x(90)91035-q
Publikováno v:
Biomedical Research. 11:313-317
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3ab543c0021bb397dc31eb3082cf3990
https://doi.org/10.2220/biomedres.11.313
https://doi.org/10.2220/biomedres.11.313
Publikováno v:
Current Eye Research. 9:39-44
The purpose of this study was to provide a direct assay for calpain and its endogenous inhibitor calpastatin in normal rabbit epithelium. Corneal epithelial extracts were fractionated by DEAE (1) chromatography on HPLC. Fractions were analyzed for ca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af24af0c049f7d6934d3d6893b3f5ab2
https://doi.org/10.3109/02713689009000053
https://doi.org/10.3109/02713689009000053