Zobrazeno 1 - 10
of 97
pro vyhledávání: '"Takashi Kuwana"'
Publikováno v:
Poultry Science, Vol 100, Iss 1, Pp 319-324 (2021)
The Green-legged Partridgelike fowl is an old Polish indigenous breed of chicken. Primordial germ cells (PGCs) are one of the best sources of precursor cells that can be used for the conservation and proliferation of the endangered breeds of bird. In
Externí odkaz:
https://doaj.org/article/5babc13004e643bc8cd3c2e626cce1df
Publikováno v:
Animals, Vol 10, Iss 10, p 1769 (2020)
The present study had two aims: (1) To develop a culture system that imitates a normal physiological environment of primordial germ cells (PGCs). There are two types of PGCs in chicken: Circulating blood (cPGCs) and gonadal (gPGCs). The culture condi
Externí odkaz:
https://doaj.org/article/e17465143d8a4897a22f5e40c6ff221e
Publikováno v:
The Journal of Poultry Science, Vol 52, Iss 3, Pp 227-231 (2015)
In the present study, chicken primordial germ cells (PGCs) were transfected with GFP gene in vitro or in vivo using the piggyBac transposon vector system. PGCs cultured for 465 days were transfected in vitro, and GFP gene expression was observed in 2
Externí odkaz:
https://doaj.org/article/1d3d76c97f8d420ea7c659579a40f462
Publikováno v:
The Journal of Poultry Science, Vol 49, Iss 2, Pp 116-123 (2012)
The present study was conducted to develop a technique for generating primordial germ cells expressing GFP gene and introducing them into gonads of recipient embryos. Primordial germ cells isolated from embryonic blood were cultured on feeder cells f
Externí odkaz:
https://doaj.org/article/90cf2e79d8054a16bf4cff0aa191a1f6
Autor:
Manabu Onuma, Takashi Kuwana
Publikováno v:
The Journal of Poultry Science, Vol 48, Iss 3, Pp 212-215 (2011)
The vertical transmission rate of Mycoplasma synoviae into primordial germ cells (PGCs) was evaluated. PGCs were collected from eggs (n=54) of M. synoviae positive chickens, with 10 to 214 PGCs obtained per egg. PGCs were mixed with Frey's medium (2m
Externí odkaz:
https://doaj.org/article/b5fa0cd0972740159a3ce470f552abf3
Publikováno v:
The Journal of Poultry Science, Vol 48, Iss 2, Pp 112-118 (2011)
Testicular gonocytes differentiate into spermatogonia and spermatogonial stem cells could be established by culturing spermatogonia in vitro. Spermatogonial stem cells differentiate exclusively into spermatozoa and thus they are very useful for genet
Externí odkaz:
https://doaj.org/article/bdb25a0211e142d787cea98f4c43be46
Publikováno v:
The Journal of Poultry Science, Vol 47, Iss 1, Pp 57-64 (2010)
The present study was carried out to develop a long-term in vitro culture system for chicken primordial germ cells (PGCs). PGCs were obtained from the blood of 2.5-day incubated embryos. GFP gene was transferred into the collected PGCs and then cultu
Externí odkaz:
https://doaj.org/article/941e5d4a7a7f4e41b8452edb6e240f56
Publikováno v:
The Journal of Poultry Science, Vol 47, Iss 1, Pp 85-88 (2010)
Morphological characteristics of symmetrically developed ovaries observed in a 17-day incubated chicken embryo were analysed. The size and shape of the left and right ovaries were almost the same, but in histological analysis they have less developed
Externí odkaz:
https://doaj.org/article/d41e5e8e25114afcba286fe33baf8683
Publikováno v:
The Journal of Poultry Science, Vol 46, Iss 1, Pp 40-45 (2009)
Chicken primordial germ cells (PGCs) circulate in the bloodstream and migrate to germinal ridges. In order to assess the migration frequencies of PGCs to left or right germinal ridges, PGCs were transferred into the bloodstream of recipient embryos a
Externí odkaz:
https://doaj.org/article/7895b0d94f2f4c7db71ad5f060b7c22f
Publikováno v:
The Journal of Poultry Science, Vol 44, Iss 4, Pp 416-425 (2007)
The present study was carried out to develop techniques for introducing exogenous DNA into primordial germ cells (PGCs) and expressing the introduced DNA efficiently in the gonads of developing chicken embryos. PGCs circulating in the bloodstream (st
Externí odkaz:
https://doaj.org/article/d49c38a9e9a842a780dc5136e12a1f7c