Rapid, simple, and effective strategy to produce monoclonal antibodies targeting native protein structures using hybridoma technology

Autor: Atsumi Sakaguchi, Yoichiro Tanaka, Eiki Shoji, Rina Sakamaki, Takao Matsuba, Yasuyuki Kurihara

BACKGROUND: Monoclonal antibodies are essential in life science research and developing antibody drugs and test drugs. Various methods have been developed to obtain monoclonal antibodies, among which hybridoma technology continues to be widely used.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0599d4f31d978fdf791b1328b80b6d79
https://doi.org/10.21203/rs.3.rs-2324136/v1

Expression of cathepsin E in pancreas: A possible tumor marker for pancreas, a preliminary report

Autor: R. Thomas Taggart, Takanobu Hayakumo, Takao Matsuba, Masamichi Hirai, Kenji Yamamoto, Kiyoshi Yasukawa, Kouji Uno, Masatsugu Nakajima, Takeshi Azuma, Shigeji Ito

Publikováno v: International Journal of Cancer. 67:492-497

Ductal cancers of the pancreas frequently express markers of gastrointestinal epithelial cells. Cathepsin E (CTSE) is a non-secretory, intracellular, but non-lysosomal proteinase found in the highest concentration in the superficial epithelial cells

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7eb5b5afd91862cccdceaae1a704b60
https://doi.org/10.1002/(sici)1097-0215(19960807)67:4<492::aid-ijc5>3.0.co

Identification by the phage-display technique of peptides that bind to H7 flagellin of Escherichia coli

Autor: Sang-Ho Baik, Shigeaki Harayama, Teruhiko Ide, Takao Matsuba

Publikováno v: Bioscience, biotechnology, and biochemistry. 67(6)

The four peptides interacting with H7 flagellin of Escherichia coli were selected from a phage display library. The library was selected four times, and the interacting phage peptides were competitively eluted with H7 flagellin. An enzyme-linked immu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a69ab5360c12dd2949c78cc7bf71ae7
https://pubmed.ncbi.nlm.nih.gov/12843662

Expression of Human Cathepsin E in Methylotrophic Yeast, Pichia Pastoris

Autor: Takao Matsuba, Takeshi Azuma, Hideo Suzuki, Kenji Yamamoto, Masayuki Yamada, Hitoshi Hori

Publikováno v: Aspartic Proteinases ISBN: 9781461357612

The human gastric cathepsin E (CTSE) was expressed in the methylotrophic yeast Pichia pastoris by placing the CTSE cDNA under the control of the methanol-inducible alcohol oxidase promoter. The human CTSE expressed in P.pastoris was efficiently secre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9cb7911fc7d53cb9483e96b8e636b018
https://doi.org/10.1007/978-1-4615-1871-6_39

Hokuriku-plus familial hypercholesterolaemia registry study: rationale and study design

Autor: Hiroshi Matsumoto, Akihiro Nomura, Hayato Tada, Hirofumi Okada, Atsushi Nohara, Masa-aki Kawashiri, Koji Maeno, Manabu Fujimoto, Shohei Yoshida, Masaya Shimojima, Toyonobu Tsuda, Mika Mori, Shin-ichiro Takashima, Takeshi Kato, Soichiro Usui, Kenji Sakata, Kenshi Hayashi, Noboru Fujino, Akihiro Inazu, Shizuko Takahara, Yasuhito Imai, Takao Matsubara, Kenji Miwa, Masanobu Namura, Hidenobu Terai, Taiji Yoshida, Tsutomu Araki, Masahiro Minamoto, Toru Aburao, Yuji Ito, Chiaki Nakanishi, Suguru Kawasaki, Yasuhiro Todo, Junji Koizumi, Yoshihito Kita, Hiroaki Shintaku, Akihiko Hodatsu, Hidekazu Ino, Toshinori Higashikata, Mutsuko Takata, Katsushi Misawa, Masato Yamaguchi, Yoshihiro Noji, Kazuo Osato, Tomohito Mabuchi, Taro Ichise, Bunji Kaku, Shoji Katsuda, Katsuharu Uchiyama, Kensuke Fujioka, Takuya Nakahashi, Tsuyoshi Nozue, Ichiro Michishita, Kazuo Usuda, Kanichi Otowa, Kazuyasu Okeie, Satoshi Hirota, Isao Aburadani, Keisuke Kurokawa, Osamu Takatori, Shunichiro Hondo, Hiroyuki Oda, Shigeo Takata, Hisayoshi Murai, Masaki Kinoshita, Hideo Nagai, Yoshiteru Sekiguchi, Satoru Sakagami, Wataru Omi, Chikara Fujita, Tatsuo Katsuki, Hiroshi Ootsuji, Atsushi Igarashi, Manabu Nakano, Seiichiro Okura, Yasuhito Mitamura, Naoki Sugimoto, Masakazu Yamamoto, Hironobu Akao, Kouji Kajinami, Masayuki Takamura

Publikováno v: BMJ Open, Vol 10, Iss 9 (2020)

Introduction Familial hypercholesterolaemia (FH) is an autosomal-dominant inherited genetic disease. It carries an extremely high cardiovascular risk associated with significantly elevated low-density lipoprotein (LDL) cholesterol. The diagnostic rat

Externí odkaz: https://doaj.org/article/917dd099aa3a44eba50203d24f5b2dba