Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Takako Awai"'
Autor:
Hiroyuki Hori, Takuya Kawamura, Takako Awai, Anna Ochi, Ryota Yamagami, Chie Tomikawa, Akira Hirata
Publikováno v:
Microorganisms, Vol 6, Iss 4, p 110 (2018)
To date, numerous modified nucleosides in tRNA as well as tRNA modification enzymes have been identified not only in thermophiles but also in mesophiles. Because most modified nucleosides in tRNA from thermophiles are common to those in tRNA from mes
Externí odkaz:
https://doaj.org/article/3198e3c4e8d84be6a8832307881726bc
Autor:
Takuya Kawamura, Anna Ochi, Hiroyuki Hori, Akira Hirata, Chie Tomikawa, Ryota Yamagami, Takako Awai
Publikováno v:
Microorganisms
Microorganisms, Vol 6, Iss 4, p 110 (2018)
Microorganisms, Vol 6, Iss 4, p 110 (2018)
To date, numerous modified nucleosides in tRNA as well as tRNA modification enzymes have been identified not only in thermophiles but also in mesophiles. Because most modified nucleosides in tRNA from thermophiles are common to those in tRNA from mes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed681846f282355dcecbcb0c9383fbb5
https://pubmed.ncbi.nlm.nih.gov/30347855
https://pubmed.ncbi.nlm.nih.gov/30347855
Autor:
Ihsanawati, Anna Ochi, Shigeyuki Yokoyama, Toru Sengoku, Takako Awai, Yoshitaka Bessho, Hiroyuki Hori, Akira Hirata
Publikováno v:
Journal of Biological Chemistry. 286:35236-35246
Archaeal and eukaryotic tRNA (N(2),N(2)-guanine)-dimethyltransferase (Trm1) produces N(2),N(2)-dimethylguanine at position 26 in tRNA. In contrast, Trm1 from Aquifex aeolicus, a hyper-thermophilic eubacterium, modifies G27 as well as G26. Here, a gel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eec2e7c40cccd9ec21266a03ba669740
https://doi.org/10.1074/jbc.m111.253641
https://doi.org/10.1074/jbc.m111.253641
Publikováno v:
Genes to Cells. 13:807-816
Recombinant Aquifex aeolicus TrmD protein has a Cys20-Cys20 disulfide bond between its two subunits. This was demonstrated by SDS-polyacrylamide gel analysis of wild-type enzyme and C20S mutant protein (in which the Cys20 residue is substituted by se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ff779898818dd663b4548c49cc1de17c
https://doi.org/10.1111/j.1365-2443.2008.01207.x
https://doi.org/10.1111/j.1365-2443.2008.01207.x
Publikováno v:
Biochemistry and Biophysics Reports
A significant challenge in the field of in vitro synthetic biology is the construction of a self-reproducing cell-free translation system, which reproduces its components, such as translation proteins, through translation and transcription by itself.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::919635d1fbdc501f015e2bfd192c90eb
https://pubmed.ncbi.nlm.nih.gov/29124177
https://pubmed.ncbi.nlm.nih.gov/29124177
Autor:
Akira Hirata, Takako Awai, Ai Kazayama, Chie Tomikawa, Eri Iwasaki, Hiroaki Kusuba, Ryota Yamagami, Takeshi Yoshida, Hiroyuki Hori
Publikováno v:
Journal of biochemistry. 158(6)
Dihydrouridine (D) is formed by tRNA dihydrouridine synthases (Dus). In mesophiles, multiple Dus enzymes bring about D modifications at several positions in tRNA. The extreme-thermophilic eubacterium Thermus thermophilus, in contrast, has only one du
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac4379797f17e1c50da41c9d1f270476
https://pubmed.ncbi.nlm.nih.gov/26112661
https://pubmed.ncbi.nlm.nih.gov/26112661
Autor:
Yoshitaka Bessho, Takashi Yokogawa, Anna Ochi, Ihsanawati, Hiroyuki Hori, Tsutomu Suzuki, Satoshi Ohno, Takako Awai, Kazuya Nishikawa, Satoshi Kimura, Shigeyuki Yokoyama, Chie Tomikawa
Publikováno v:
The Journal of biological chemistry. 284(31)
Transfer RNA (N2,N2-guanine)-dimethyltransferase (Trm1) catalyzes N2,N2-dimethylguanine formation at position 26 (m(2)(2)G26) in tRNA. In the reaction, N2-guanine at position 26 (m(2)G26) is generated as an intermediate. The trm1 genes are found only
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc3504421c5154eab881382296003dd3
https://pubmed.ncbi.nlm.nih.gov/19491098
https://pubmed.ncbi.nlm.nih.gov/19491098
Publikováno v:
Genes to cells : devoted to molecularcellular mechanisms. 13(8)
Recombinant Aquifex aeolicus TrmD protein has a Cys20-Cys20 disulfide bond between its two subunits. This was demonstrated by SDS-polyacrylamide gel analysis of wild-type enzyme and C20S mutant protein (in which the Cys20 residue is substituted by se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cbeb7676cfaafb5ab79ca78b175e9977
https://pubmed.ncbi.nlm.nih.gov/18651851
https://pubmed.ncbi.nlm.nih.gov/18651851
Publikováno v:
Nucleic Acids Symposium Series. 49:303-304
Aquifex aeolicus is a hyper-thermophilic eubacterium, which probably diverged at the earliest period from the other bacteria on the evolution of life. Therefore, analyses of RNA modification enzymes of this bacterium may supply important information
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc38188ebb44edd195ff7e017f68c5bd
https://doi.org/10.1093/nass/49.1.303
https://doi.org/10.1093/nass/49.1.303
Autor:
Hiroaki Kusuba, Takeshi Yoshida, Eri Iwasaki, Takako Awai, Ai Kazayama, Akira Hirata, Chie Tomikawa, Ryota Yamagami, Hiroyuki Hori
Publikováno v:
Journal of Biochemistry; Dec2015, Vol. 158 Issue 6, p513-521, 9p