Zobrazeno 1 - 10
of 103
pro vyhledávání: '"Takahiro Hohsaka"'
Autor:
Takashi Tamura, Masaaki Inoue, Yuji Yoshimitsu, Ichihiko Hashimoto, Noriyuki Ohashi, Kyosuke Tsumura, Koo Suzuki, Takayoshi Watanabe, Takahiro Hohsaka
Publikováno v:
Bulletin of the Chemical Society of Japan. 95:359-366
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a9a43abc177014d3197667410b713b85
https://doi.org/10.1246/bcsj.20210409
https://doi.org/10.1246/bcsj.20210409
Autor:
Hiroki Nakai, Kinuka Isshiki, Masato Hattori, Hiromasa Maehira, Tatsumi Yamaguchi, Keiko Masuda, Yoshihiro Shimizu, Takayoshi Watanabe, Takahiro Hohsaka, Wataru Shihoya, Osamu Nureki, Yasuhiko Kato, Hajime Watanabe, Tomoaki Matsuura
Publikováno v:
Analytical chemistry. 94(9)
Engineering G-protein-coupled receptors (GPCRs) for improved stability or altered function is of great interest, as GPCRs consist of the largest protein family, are involved in many important signaling pathways, and thus, are one of the major drug ta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7e8348b28aea78def4e575c63aa51ad
https://pubmed.ncbi.nlm.nih.gov/35188389
https://pubmed.ncbi.nlm.nih.gov/35188389
Autor:
Toru Ezure, Kei Nanatani, Yoko Sato, Satomi Suzuki, Keishi Aizawa, Satoshi Souma, Masaaki Ito, Takahiro Hohsaka, Gunnar von Heijine, Toshihiko Utsumi, Keietsu Abe, Eiji Ando, Nobuyuki Uozumi
Publikováno v:
PLoS ONE, Vol 9, Iss 12, p e112874 (2014)
Cell-free protein synthesis is a powerful method to explore the structure and function of membrane proteins and to analyze the targeting and translocation of proteins across the ER membrane. Developing a cell-free system based on cultured cells for t
Externí odkaz:
https://doaj.org/article/852e5f81e4304621b69e70c65a6d5cae
Publikováno v:
Chemical Physics Letters. 698:223-226
We performed metadynamics molecular dynamics simulations to reveal mechanism of antigen-dependent fluorescence response observed for site-specifically fluorescent-labeled single-chain antibody against c-Myc peptide antigen. We found that V H and V L
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ef70c64edd457a0a17ae1da8fc9128ae
https://doi.org/10.1016/j.cplett.2018.03.011
https://doi.org/10.1016/j.cplett.2018.03.011
Autor:
Tadashi Nakaji-Hirabayashi, Takayoshi Watanabe, Kazuaki Matsumura, Takahiro Hohsaka, Sana Ahmed
Publikováno v:
ACS Biomaterials Science and Engineering. 3(8):1677-1689
Physical methodologies such as electroporation and the gene-gun technology have been widely used for transfection; however, their applicability is limited because they lead to cell damage and low cell viability. Therefore, to address these limitation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e98d230a4cb2adab4563c9ab51d98fdd
http://hdl.handle.net/10119/15875
http://hdl.handle.net/10119/15875
Autor:
Shogo Yoshimoto, Katsutoshi Hori, Tomoaki Matsuura, Takahiro Hohsaka, Kosaku Noba, Masahito Ishikawa, Atsuko Uyeda, Takayoshi Watanabe
The bacterial cell surface structure has important roles for various cellular functions. However, research on reconstituting bacterial cell surface structures is limited. This study aimed to bottom-up create a cell-sized liposome covered with AtaA, t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d1bda207c071dd01b82b1611d57988d
https://doi.org/10.26434/chemrxiv.9735161.v1
https://doi.org/10.26434/chemrxiv.9735161.v1
Publikováno v:
Bulletin of the Chemical Society of Japan. 89:573-580
Fluorescence ratio probes are useful tools for quantitative detection of target molecules even if the concentration of probe molecules is unknown. However, a general and widely applicable method fo...
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a36929cb8204fc038bb80d620c6fb04e
https://doi.org/10.1246/bcsj.20150384
https://doi.org/10.1246/bcsj.20150384
Publikováno v:
Journal of Molecular Recognition. 29:485-491
In the present study, a novel molecular biosensor system model was designed by using a couple of the fluorescent unnatural mutant streptavidin and the carbazole-labeled biotin. BODIPY-FL-aminophenylalanine (BFLAF), a fluorescent unnatural amino acid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::200a4ed8ce1bd5fce9f405bf398ccb5b
https://doi.org/10.1002/jmr.2548
https://doi.org/10.1002/jmr.2548
Publikováno v:
Synthetic Biology
Membranes of living cells possess asymmetry. The inner and outer leaflets of the membrane consist of different phospholipid compositions, which are known to affect the function of membrane proteins, and the loss of the asymmetry has been reported to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41e2ad3e36db1cd2434862697a0d481f
https://doi.org/10.1093/synbio/ysy007
https://doi.org/10.1093/synbio/ysy007
Autor:
Kensaku Sakamoto, Kazumasa Ohtake, Takayoshi Watanabe, Tatsuo Yanagisawa, Takahiro Hohsaka, Atsushi Yamaguchi, Yoshihisa Fujiwara, Takayoshi Matsuda, Shigeyuki Yokoyama
Publikováno v:
Bioconjugate Chemistry. 27:198-206
Z-Lysine (ZLys) is a lysine derivative with a benzyloxycarbonyl group linked to the ε-nitrogen. It has been genetically encoded with the UAG stop codon, using the pair of an engineered variant of pyrrolysyl-tRNA synthetase (PylRS) and tRNA(Pyl). In
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c6e80d13af853290121e025f97e2497
https://doi.org/10.1021/acs.bioconjchem.5b00602
https://doi.org/10.1021/acs.bioconjchem.5b00602