Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Takahide Yokoi"'
Autor:
Junko Tanaka, Tatsuo Nakagawa, Kunio Harada, Chigusa Morizane, Hidenori Tanaka, Satoshi Shiba, Akihiro Ohba, Susumu Hijioka, Erina Takai, Shinichi Yachida, Yoshio Kamura, Takeshi Ishida, Takahide Yokoi, Chihiro Uematsu
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)
Abstract A highly sensitive and highly multiplexed quantification technique for nucleic acids is necessary to predict and evaluate cancer treatment by liquid biopsy. Digital PCR (dPCR) is a highly sensitive quantification technique, but conventional
Externí odkaz:
https://doaj.org/article/64bb1301ddb449c4b50b7765f409a5e0
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
Abstract To achieve DNA sequencing with solid-state nanopores, the speed of the DNA in the nanopore must be controlled to obtain sequence-specific signals. In this study, we fabricated a nanopore-sensing system equipped with a DNA motion controller.
Externí odkaz:
https://doaj.org/article/e40174e1af4f461b9338e84bbeab5af8
Autor:
Tatsuo, Nakagawa, Junko, Tanaka, Kunio, Harada, Akiko, Shiratori, Yuzuru, Shimazaki, Takahide, Yokoi, Chihiro, Uematsu, Yoshinobu, Kohara
Publikováno v:
Analytical chemistry. 92(17)
Digital PCR (dPCR) is a promising method for performing liquid biopsies that quantifies nucleic acids more sensitively than real-time PCR. However, dPCR shows large fluctuations in the fluorescence intensity of droplets or wells due to insufficient P
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f5703358f72af26959b687d50f7cb494
https://pubmed.ncbi.nlm.nih.gov/32786457
https://pubmed.ncbi.nlm.nih.gov/32786457
Autor:
Kenichi Takeda, Yusuke Goto, Takahide Yokoi, Rena Akahori, Fujioka Michiru, Tatsuo Nakagawa, Itaru Yanagi, Kazuma Matsui, Takeshi Ishida
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
Scientific Reports
Scientific Reports
Nanopore-based biosensors have attracted attention as highly sensitive microscopes for detecting single molecules in aqueous solutions. However, the ionic current noise through a nanopore degrades the measurement accuracy. In this study, the magnitud
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce5fcd9876f212a1d0fe47712aaba177
https://doi.org/10.1038/s41598-020-65530-y
https://doi.org/10.1038/s41598-020-65530-y
Autor:
Tatsuo Nakagawa, Kunio Harada, Junko Tanaka, Akiko Shiratori, Masao Kamahori, Takahide Yokoi, Yoshinobu Kohara, Chihiro Uematsu, Yuzuru Shimazaki
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
Scientific Reports
Scientific Reports
Digital PCR (dPCR) has been developed as a method that can quantify nucleic acids more sensitively than real-time PCR. However, dPCR exhibits large fluctuations in the fluorescence intensity of the compartment, resulting in low accuracy. The main cau
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47129e377bf63d079bd0b049b5d302cf
https://doi.org/10.1038/s41598-019-38822-1
https://doi.org/10.1038/s41598-019-38822-1
Publikováno v:
Lab on a chip. 17(13)
A five-color fluorescence-detection system for eight-channel plastic-microchip electrophoresis was developed. In the eight channels (with effective electrophoretic lengths of 10 cm), single-stranded DNA fragments were separated (with single-base reso
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c6bb6821d0942d2d1b31413ad216547
https://pubmed.ncbi.nlm.nih.gov/28585967
https://pubmed.ncbi.nlm.nih.gov/28585967
Autor:
Yoshimitsu Yanagawa, Kenichi Takeda, T. Iwasaki, Mayu Aoki, Kazuma Matsui, Itaru Yanagi, Yoshikazu Nara, Rena Akahori, Yusuke Goto, Takahide Yokoi
Publikováno v:
Extended Abstracts of the 2016 International Conference on Solid State Devices and Materials.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f952610c2b1e9f33b877f1950e6978ed
https://doi.org/10.7567/ssdm.2016.h-2-01
https://doi.org/10.7567/ssdm.2016.h-2-01
Publikováno v:
Scientific Reports
The practical use of solid-state nanopores for DNA sequencing requires easy fabrication of the nanopores, reduction of the DNA movement speed and reduction of the ionic current noise. Here, we report an integrated nanopore platform with a nanobead st
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f17bc3e7a5fcd451ac57a09f5bb17564
https://doi.org/10.1038/srep31324
https://doi.org/10.1038/srep31324
Autor:
Kazuma Matsui, Yusuke Goto, Takahide Yokoi, Naomi Manri, Rena Akahori, Kenichi Takeda, Yoshikazu Nara, Mayu Aoki, Tomio Iwasaki, Itaru Yanagi
Publikováno v:
2015 IEEE International Electron Devices Meeting (IEDM).
This paper summarizes the latest developments regarding solid-state nanopores achieved in our laboratory. For direct DNA sequencing with solid-state nanopores, a simple method has been developed to precisely fabricate nanopores with diameters of 1 to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7a54d2ebc7774a460a2b443a2d424170
https://doi.org/10.1109/iedm.2015.7409692
https://doi.org/10.1109/iedm.2015.7409692
Publikováno v:
Biochemical and Biophysical Research Communications. 409:786-791
Adipogenesis is a well-characterized cell differentiation process. A large body of evidence has revealed the core transcription factors and signaling pathways that govern adipogenesis, but cross-talks between these cellular signals and its functional
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f76d432a9a41ddecf1413fd389a6d38
https://doi.org/10.1016/j.bbrc.2011.05.089
https://doi.org/10.1016/j.bbrc.2011.05.089