Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Takaaki Yokomine"'
Autor:
Takaaki Yokomine, Masahiro Sato, Haiying Chi, Mariko Shinohara, Kazuchika Miyoshi, Sonshin Takao, Mitsutoshi Yoshida
Publikováno v:
Journal of Reproduction and Development. 58:69-76
RNA interference (RNAi) technology using small interfering RNAs (siRNA) has been widely used as a powerful tool to knock down gene expression in various organisms. In pig preimplantation embryos, no attempt to suppress the target gene expression with
Autor:
Masahiro Sato, Takaaki Yokomine, Takehiro Himaki, Sonshin Takao, Kazuchika Miyoshi, Mitsutoshi Yoshida
Publikováno v:
Animal Science Journal. 81:558-563
The present study was carried out to examine the effects of post-activation treatment of trichostatin A (TSA), a histone deacetylase inhibitor, on in vitro development and transgene function of somatic cell nuclear transfer (SCNT) embryos derived fro
Autor:
Masahiro Sato, Takaaki Yokomine, Tadaaki Maehara, Masato Ohtsuka, Masayuki Ishihara, Satoshi Watanabe, Shingo Nakamura
Publikováno v:
Molecular Reproduction and Development. 75:1085-1093
Attempts to image reporter gene expression driven by weak promoters are often hampered by the poor transcriptional activity of such promoters. Most tissue-specific promoters are weak compared with stronger but constitutively expressing viral promoter
Autor:
Asuka Nakayama, Shyuichiro Matsubara, Takaaki Yokomine, Mitsutoshi Yoshida, Mariko Shinohara, Eri Akasaka, Sonshin Takao, Masahiro Sato
Publikováno v:
Cloning and Stem Cells. 9:523-534
Porcine embryonic fibroblasts (PEF) are important as donor cells for nuclear transfer for generation of genetically modified pigs. In this study, we determined an optimal protocol for transfection of PEF with the Amaxa Nucleofection system, which dir
Autor:
Takashi Muramatsu, Takaaki Yokomine, Masako Misawa, Satoshi Watanabe, Takashi Matsuzaki, Takayuki Sakurai, Masahiro Sato
Publikováno v:
Glycobiology. 18:9-19
The alphaGal epitope (Galalpha1-3Gal) is a sugar structure expressed on the cell surface of almost all organisms except humans and old-world-monkeys, which express natural anti-alphaGal antibodies. The presence of these antibodies elicits a hyper acu
Publikováno v:
Cytogenetic and Genome Research. 113:75-80
DNA methylation plays an essential role in genomic imprinting observed in eutherian mammals and marsupials. In mouse, one of the two de novo DNA methyltransferases, Dnmt3a, and a related protein, Dnmt3L have been shown to be essential for imprint est
Publikováno v:
Molecular Reproduction and Development. 73:116-122
The DNA methyltransferase-like protein Dnmt3L is necessary for the establishment of genomic imprints in oogenesis and for normal spermatogenesis (Bourc'his et al., 2001; Hata et al., 2002). Also, a paternally imprinted gene, H19, loses DNA methylatio
Autor:
Yoshiyuki Sakaki, Masahira Hattori, Atsushi Toyoda, Yoh Ichi Matsuda, Hiroyuki Sasaki, Masaoki Tsudzuki, Hisao Shirohzu, Takaaki Yokomine, Hisakazu Iwama, Kazuho Ikeo, Tetsuya Hori, Wahyu Purbowasito, Shigeki Mizuno
Publikováno v:
Genome Research. 15:154-165
Previous studies revealed thatIgf2andMpr/Igf2rare imprinted in eutherian mammals and marsupials but not in monotremes or birds.Igf2lies in a large imprinted cluster in eutherians, and its imprinting is regulated by long-range mechanisms. As a step to
Autor:
Takaaki Yokomine, Atsushi Toyoda, Reiko Kato, Chikako Suda, Hisao Shirohzu, Hiroyuki Sasaki, Masahira Hattori, Chiyoko Sato, Yoshiyuki Sakaki, Katsuzumi Okumura, Tsunehiro Mukai, Wahyu Purbowasito
Publikováno v:
DNA Research. 11:325-334
Mammalian genes subject to genomic imprinting often form clusters and are regulated by long-range mechanisms. The distal imprinted domain of mouse chromosome 7 is orthologous to the Beckwith-Wiedemann syndrome domain in human chromosome 11p15.5 and c
Autor:
Hiroyuki Sasaki, Takashi Sado, Ken Tsutsui, Wahyu Purbowasito, Takaaki Yokomine, Chikako Suda, Mohamad Zubair
Publikováno v:
DNA Research. 11:391-407
Mammalian imprinted genes, which are expressed from only one of the parental alleles, have a tendency to form clusters and are regulated by long-range mechanisms. Nuclear matrix-attachment regions (MARs), the anchor points of loop domains, are involv