Výsledky vyhledávání - "Takaaki Horie"

Akademický článek

Establishment of a conditional TALEN system using the translational enhancer dMac3 and an inducible promoter activated by glucocorticoid treatment to increase the frequency of targeted mutagenesis in plants.

Autor: Hitomi Onodera, Saeko Shingu, Mariko Ohnuma, Takaaki Horie, Miho Kihira, Hiroaki Kusano, Hiroshi Teramura, Hiroaki Shimada

Publikováno v: PLoS ONE, Vol 13, Iss 12, p e0208959 (2018)

Transcription activator-like effector nuclease (TALEN) is an artificial nuclease that causes DNA cleavage at the target site and induces few off-target reactions because of its high sequence specificity. Powerful and variable tools using TALENs can b

Externí odkaz: https://doaj.org/article/aa85e7a0cc0e45f8ad619d897ed3984f

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Autor: Mariko Ohnuma, Hiroaki Shimada, Hitomi Onodera, Takaaki Horie, Hiroaki Kusano, Hiroshi Teramura, Miho Kihira, Saeko Shingu

Publikováno v: PLoS ONE, Vol 13, Iss 12, p e0208959 (2018)
PLoS ONE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e344e9475c11cb1fcb13658fed827e0
https://doaj.org/article/aa85e7a0cc0e45f8ad619d897ed3984f

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Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato

Autor: Kenji Asano, Miho Kihira, Naoyuki Umemoto, Kazufumi Yazaki, Toshiya Muranaka, Kou Fukumoto, Hitomi Onodera, Hiroaki Shimada, Dai Ichinosawa, Hiroshi Teramura, Takahiro Noda, Takaaki Horie, Takahiro Asahi, Hiroaki Kusano, Hiromi Mutsuro-Aoki, Mariko Ohnuma

Publikováno v: SC30201902220013
NARO成果DBa
Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
Scientific Reports

CRISPR/Cas9 is a programmable nuclease composed of the Cas9 protein and a guide RNA (gRNA) molecule. To create a mutant potato, a powerful genome-editing system was required because potato has a tetraploid genome. The translational enhancer dMac3, co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8dd418d8d6041ee46229a9d24d7ddc0
http://id.nii.ac.jp/1578/00003372/

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