Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Taishi Sugawara"'
Characterization of the β-d-Glucopyranoside Binding Site of the Human Bitter Taste Receptor hTAS2R16
Autor:
Taishi Sugawara, Shinji Matsuo, Yoshiro Ishimaru, Keiko Abe, Takanobu Sakurai, Takuya Kobayashi, Takumi Misaka, Tomiko Asakura, Masaji Ishiguro, Keisuke Ito, Katsuyoshi Masuda
Publikováno v:
The Journal of Biological Chemistry
G-protein-coupled receptors mediate the senses of taste, smell, and vision in mammals. Humans recognize thousands of compounds as bitter, and this response is mediated by the hTAS2R family, which is one of the G-protein-coupled receptors composed of
Autor:
So Iwata, Keiko Abe, Taishi Sugawara, Keisuke Ito, Natsuko Tokuda, Hidetsugu Asada, Norimichi Nomura, Takeshi Murata, Takami Yurugi-Kobayashi, Mitsunori Shiroishi, Takumi Misaka, Tatsuro Shimamura, Takuya Kobayashi
Publikováno v:
Biochemical and Biophysical Research Communications. 382:704-710
Human TAS2 receptors (hTAS2Rs) perceive bitter tastants, but few studies have explored the structure-function relationships of these receptors. In this paper, we report our trials on the large-scale preparations of hTAS2Rs for structural analysis. Tw
Autor:
Hidetsugu Asada, Mitsunori Shiroishi, Keisuke Ito, So Iwata, Tatsuro Shimamura, Takeshi Murata, Kiyohiko Igarashi, Takuya Kobayashi, Norimichi Nomura, Taishi Sugawara, Takami Yurugi-Kobayashi, Natsuko Tokuda
Publikováno v:
Protein expression and purification. 79(1)
Anion exchangers are membrane proteins that have been identified in a wide variety of species, where they transport Cl− and HCO 3 - across the cell membrane. In this study, we cloned an anion-exchange protein from the genome of the basidiomycete Ph
Autor:
Hidetsugu Asada, Takanori Nakane, Norimichi Nomura, Takami Yurugi-Kobayashi, Tatsuya Haga, So Iwata, Tomoko Uemura, Taishi Sugawara, Takuya Kobayashi, Hirokazu Tsujimoto, Keisuke Ito, Takeshi Murata, Mitsunori Shiroishi, Tatsuro Shimamura
Publikováno v:
Microbial Cell Factories
Microbial Cell Factories, Vol 10, Iss 1, p 24 (2011)
Microbial Cell Factories, Vol 10, Iss 1, p 24 (2011)
Background Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coup
Autor:
Akiko Shimizu-Ibuka, Hidetsugu Asada, Ayako Koizumi, Takami Yurugi-Kobayashi, Mitsunori Shiroishi, Takumi Misaka, So Iwata, Keiko Abe, Taishi Sugawara, Takuya Kobayashi, Tatsuro Shimamura, Keisuke Ito, Ken Ichiro Nakajima, Tomiko Asakura
Publikováno v:
Biotechnology letters. 33(1)
Purpose of work Soluble protein expression is an important first step during various types of protein studies. Here, we present the screening strategy of secretable mutant. The strategy aimed to identify those cysteine residues that provoke protein m
Autor:
Masaji Ishiguro, Keisuke Ito, Ken Ichiro Nakajima, Takuya Kobayashi, Akiko Shimizu-Ibuka, Takumi Misaka, So Iwata, Katsuyoshi Masuda, Tomiko Asakura, Hidetsugu Asada, Ayako Koizumi, Keiko Abe, Mitsunori Shiroishi, Tatsuro Shimamura, Takami Yurugi-Kobayashi, Taishi Sugawara
Publikováno v:
Biochimica et biophysica acta. 1800(9)
Background Miraculin (MCL) is a taste-modifying protein that converts sourness into sweetness. The molecular mechanism underlying the taste-modifying action of MCL is unknown. Methods Here, a yeast expression system for MCL was constructed to acceler
Autor:
Hirokazu Tsujimoto, Norimichi Nomura, Takeshi Murata, Keisuke Ito, Naoko Katsuta, Takami Yurugi-Kobayashi, Mitsunori Shiroishi, Kazuko Haga, Takuya Kobayashi, Taishi Sugawara, Tatsuro Shimamura, Saeko Funamoto, Tatsuya Haga, Natsuko Tokuda, Hidetsugu Asada, So Iwata
Publikováno v:
Biochemical and biophysical research communications. 380(2)
N -linked glycosylation is the most common post-translational modification of G-protein-coupled receptors (GPCRs) and is correlated to the localization and function of the receptors depending on each receptor. However, heterogeneity of glycosylation
Autor:
So Iwata, Takeshi Murata, Hisayoshi Makyio, Mitsunori Shiroishi, Tatsuro Shimamura, Azusa Kurokawa, Takuya Kobayashi, Natsuko Tokuda, Norimichi Nomura, Keiko Abe, Takumi Misaka, Takami Yurugi-Kobayashi, Taishi Sugawara, Keisuke Ito
Publikováno v:
Biochemical and biophysical research communications. 371(4)
Crystallization of eukaryotic membrane proteins is a challenging, iterative process. The protein of interest is often modified in an attempt to improve crystallization and diffraction results. To accelerate this process, we took advantage of a GFP-fu