The asparagine 533 residue in the outer pore loop region of the mouse PKD2L1 channel is essential for its voltage‐dependent inactivation

Autor: Takahiro Shimizu, Taiga Higuchi, Toshihiro Toba, Chie Ohno, Takuto Fujii, Bernd Nilius, Hideki Sakai

Publikováno v: FEBS Open Bio, Vol 7, Iss 9, Pp 1392-1401 (2017)

Voltage‐dependent inactivation of ion channels contributes to the regulation of the membrane potential of excitable cells. Mouse polycystic kidney disease 2‐like 1 (PKD2L1) forms voltage‐dependent nonselective cation channels, which are activat

Externí odkaz: https://doaj.org/article/9b4c822774004391a51b5aa8190bee86

Functional coupling of chloride–proton exchanger ClC-5 to gastric H+,K+-ATPase

Autor: Yuji Takahashi, Takuto Fujii, Kyosuke Fujita, Takahiro Shimizu, Taiga Higuchi, Yoshiaki Tabuchi, Hisato Sakamoto, Ichiro Naito, Koji Manabe, Shinichi Uchida, Sei Sasaki, Akira Ikari, Kazuhiro Tsukada, Hideki Sakai

Publikováno v: Biology Open, Vol 3, Iss 1, Pp 12-21 (2013)

Summary It has been reported that chloride–proton exchanger ClC-5 and vacuolar-type H+-ATPase are essential for endosomal acidification in the renal proximal cells. Here, we found that ClC-5 is expressed in the gastric parietal cells which secrete

Externí odkaz: https://doaj.org/article/e014b0c1547841c2af3174adf66d8b7c

A microsensing system for the in vivo real-time detection of local drug kinetics

Autor: Seishiro Sawamura, Yasuaki Einaga, Yuya Ishii, Kazuya Maeda, Takamasa Yoshida, Takeru Ota, Ian Findlay, Hiroyuki Kusuhara, Karin Hori, Katsumi Doi, Hiroshi Hibino, Fumiaki Nin, Madoka Takai, Shizuo Komune, Yamato Sano, Genki Ogata, Taiga Higuchi, Kai Asai

Publikováno v: Nature Biomedical Engineering
Nature Biomedical Engineering, Nature Publishing Group, 2017, 1 (8), pp.654-666. ⟨10.1038/s41551-017-0118-5⟩

Real-time recording of the kinetics of systemically administered drugs in in vivo microenvironments may accelerate the development of effective medical therapies. However, conventional methods require considerable analyte quantities, have low samplin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a65eb023cb056d0a8361a77463adab78
https://doi.org/10.1038/s41551-017-0118-5

Time-controllable Nkcc1 knockdown replicates reversible hearing loss in postnatal mice

Autor: Ming Xu, Kaoru Ogawa, Junichi Nabekura, Fumiaki Nin, Taiga Higuchi, Takahisa Watabe, Mitsuo P. Sato, Kenji F. Tanaka, Masatsugu Masuda, Hiroshi Hibino, Karin Hori, Miho Watanabe

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Scientific Reports

Identification of the causal effects of specific proteins on recurrent and partially reversible hearing loss has been difficult because of the lack of an animal model that provides reversible gene knockdown. We have developed the transgenic mouse lin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d265afb452e4a2d67a87b756fa816f2
https://doi.org/10.1038/s41598-017-13997-7

Functional coupling of chloride–proton exchanger ClC-5 to gastric H+,K+-ATPase

Autor: Kazuhiro Tsukada, Yuji Takahashi, Kyosuke Fujita, Sei Sasaki, Hideki Sakai, Koji Manabe, Shinichi Uchida, Takahiro Shimizu, Takuto Fujii, Hisato Sakamoto, Akira Ikari, Ichiro Naito, Yoshiaki Tabuchi, Taiga Higuchi

Publikováno v: Biology Open
Biology Open, Vol 3, Iss 1, Pp 12-21 (2013)

Summary It has been reported that chloride–proton exchanger ClC-5 and vacuolar-type H+-ATPase are essential for endosomal acidification in the renal proximal cells. Here, we found that ClC-5 is expressed in the gastric parietal cells which secrete

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aece87c135469a1dfaa9753ef6fd0956
https://doi.org/10.1242/bio.20136205