Application of Single Molecule Fluorescence Microscopy to Characterize the Penetration of a Large Amphiphilic Molecule in the Stratum Corneum of Human Skin

Autor: Zahra Afraz, Alexander Boreham, Janna Frombach, Ulrike Alexiev, Jens Balke, Tai-Yang Kim, Ulrike Blume-Peytavi, Fiorenza Rancan, Sabrina Hadam, Annika Vogt, Alexander Wolf, Pierre Volz

Publikováno v: International Journal of Molecular Sciences, Vol 16, Iss 4, Pp 6960-6977 (2015)
International Journal of Molecular Sciences
Volume 16
Issue 4
Pages 6960-6977

We report here on the application of laser-based single molecule total internal reflection fluorescence microscopy (TIRFM) to study the penetration of molecules through the skin. Penetration of topically applied drug molecules is often observed to be

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7709eca058566f33da734e2cee1bc40
http://www.mdpi.com/1422-0067/16/4/6960

A simulation-guided fluorescence correlation spectroscopy tool to investigate the protonation dynamics of cytochrome c oxidase

Autor: Ulrike Alexiev, Kristina Kirchberg, Constantin Schneider, Tai-Yang Kim, Alexander Wolf, Pierre Volz

Fluorescence correlation spectroscopy (FCS) is a single molecule based technique to temporally resolve rate-dependent processes by correlating the fluorescence fluctuations of individual molecules traversing through a confocal volume. In addition, ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c41ef6e04774c60ce659b80da884096
https://refubium.fu-berlin.de/handle/fub188/21170

Conformational dynamics of helix 8 in the GPCR rhodopsin controls arrestin activation in the desensitization process

Autor: Ulrike Alexiev, Ramona Schlesinger, Martina Möller, Tai-Yang Kim, Darko Skegro, Georg Büldt, Joachim Granzin, Kristina Kirchberg, Gayathri Dasara Raju

Publikováno v: Proceedings of the National Academy of Sciences. 108:18690-18695

Arrestins are regulatory molecules for G-protein coupled receptor function. In visual rhodopsin, selective binding of arrestin to the cytoplasmic side of light-activated, phosphorylated rhodopsin (P-Rh*) terminates signaling via the G-protein transdu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93cd2a627e1210548aad7d7cbb29a1d1
https://doi.org/10.1073/pnas.1015461108

Exploiting Fluorescence Lifetime Plasticity in FLIM: Target Molecule Localization in Cells and Tissues

Autor: Rainer Haag, Alexander Boreham, Viola Spahn, Lars Mundhenk, Achim D. Gruber, Tai-Yang Kim, Ulrike Alexiev, Christoph Stein, Kai Licha, Pia Welker

Publikováno v: ACS medicinal chemistry letters. 2(10)

The mechanisms of drug–receptor interactions and the controlled delivery of drugs via biodegradable and biocompatible nanoparticulate carriers are active research fields in nanomedicine. Many clinically used drugs target G-protein coupled receptors

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e471a54e91d0bb75db89b20ed167b951
https://pubmed.ncbi.nlm.nih.gov/24900259

Functional interaction structures of the photochromic retinal protein rhodopsin

Autor: Sebastian Haase, Kristina Kirchberg, Tai-Yang Kim, Ulrike Alexiev

Publikováno v: Photochemicalphotobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology. 9(2)

We studied functional interaction structures of the vertebrate membrane photoreceptor rhodopsin containing retinal as a chromophore. Using time-resolved fluorescence depolarization we analyzed real-time dynamics and conformational changes of the cyto

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be6562fc045af563e7b34b2e0876bf7e
https://pubmed.ncbi.nlm.nih.gov/20126799