Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Tae Kwang Ha"'
Autor:
Tae Kwang Ha, Andreu Òdena, Karen Julie la Cour Karottki, Che Lin Kim, Hooman Hefzi, Gyun Min Lee, Helene Faustrup Kildegaard, Lars K. Nielsen, Lise Marie Grav, Nathan E. Lewis
The dominant method for generating Chinese hamster ovary (CHO) cell lines that produce high titers of biotherapeutic proteins utilizes selectable markers such as dihydrofolate reductase (Dhfr) or glutamine synthetase (Gs), alongside inhibitory compou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0f1bc0d7cf24d24464749471b5974c5
https://doi.org/10.22541/au.165606842.29880741/v1
https://doi.org/10.22541/au.165606842.29880741/v1
Publikováno v:
Metabolic Engineering. 57:182-192
Sialic acid, a terminal monosaccharide present in N-glycans, plays an important role in determining both the in vivo half-life and the therapeutic efficacy of recombinant glycoproteins. Low sialylation levels of recombinant human erythropoietin (rhEP
Autor:
Anne Mathilde Davy, Helene Faustrup Kildegaard, Tune Wulff, Hooman Hefzi, Johnny Arnsdorf, Sara Pereira, Tae Kwang Ha, Lasse Ebdrup Pedersen, Mikael Rørdam Andersen, Daniel Ley
Publikováno v:
Ley, D, Pereira, S, Pedersen, L E, Arnsdorf, J, Hefzi, H, Davy, A M, Kwang Ha, T, Wulff, T, Kildegaard, H F & Andersen, M R 2019, ' Reprogramming AA catabolism in CHO cells with CRISPR/Cas9 genome editing improves cell growth and reduces byproduct secretion ', Metabolic Engineering, vol. 56, pp. 120-129 . https://doi.org/10.1016/j.ymben.2019.09.005
Chinese hamster ovary (CHO) cells are the preferred host for producing biopharmaceuticals. Amino acids are biologically important precursors for CHO metabolism; they serve as building blocks for proteogenesis, including synthesis of biomass and recom
Publikováno v:
Cell Culture Engineering
Publikováno v:
Biotechnology advances. 54
Chinese hamster ovary (CHO) cells are the most widely used mammalian host cells for the commercial production of therapeutic proteins. Fed-batch culture is widely used to produce therapeutic proteins, including monoclonal antibodies, because of its o
Publikováno v:
Kwang Ha, T, Hansen, A H, Kildegaard, H F & Min Lee, G 2019, ' BiP Inducer X: An ER Stress Inhibitor for Enhancing Recombinant Antibody Production in CHO Cell Culture ', Biotechnology Journal . https://doi.org/10.1002/biot.201900130
Prolonged endoplasmic reticulum (ER) stress reduces protein synthesis and induces apoptosis in mammalian cells. When dimethyl sulfoxide (DMSO), a specific monoclonal antibody productivity (qmAb )-enhancing reagent, is added to recombinant Chinese ham
Autor:
Jae Seong Lee, Mojtaba Samoudi, Helene Faustrup Kildegaard, Gyun Min Lee, Tae Kwang Ha, Bernhard O. Palsson, Jin Hyoung Park, Nathan E. Lewis
Publikováno v:
ACS synthetic biology, vol 7, iss 12
Lee, J S, Park, J H, Kwang Ha, T, Samoudi, M, Lewis, N E, Palsson, B O, Kildegaard, H F & Min Lee, G 2018, ' Revealing key determinants of clonal variation in transgene expression in recombinant CHO cells using targeted genome editing ', A C S Synthetic Biology, vol. 7, no. 12, pp. 2867-2878 . https://doi.org/10.1021/acssynbio.8b00290
Lee, J S, Park, J H, Kwang Ha, T, Samoudi, M, Lewis, N E, Palsson, B O, Kildegaard, H F & Min Lee, G 2018, ' Revealing key determinants of clonal variation in transgene expression in recombinant CHO cells using targeted genome editing ', A C S Synthetic Biology, vol. 7, no. 12, pp. 2867-2878 . https://doi.org/10.1021/acssynbio.8b00290
Generation of recombinant Chinese hamster ovary (rCHO) cell lines is critical for the production of therapeutic proteins. However, the high degree of phenotypic heterogeneity among generated clones, referred to as clonal variation, makes the rCHO cel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::349431bd58ba933d67d02cb46964dce0
https://europepmc.org/articles/PMC6535434/
https://europepmc.org/articles/PMC6535434/
Publikováno v:
Biotechnology and Bioprocess Engineering. 21:667-675
Lithium chloride (LiCl), which induces cell cycle arrest at G2/M phase, is known as a specific production rate (qp)-enhancing additive in recombinant Chinese hamster ovary (CHO) cell culture. To determine the potential of LiCl as a chemical additive
Autor:
Gyun Min Lee, Tae Kwang Ha
Publikováno v:
Pharmaceutical Bioprocessing. 3:249-261
The biopharmaceutical market is driven by the steady increase in demand for therapeutic proteins produced in mammalian cells. Glutamine is a main nitrogen source and also a main energy source with glucose in mammalian cell cultures for therapeutic pr
Publikováno v:
Biotechnology and Bioengineering. 112:1583-1593
To understand the effects of ammonium on N-glycosylation, recombinant Chinese hamster ovary (rCHO) cells that produce the Fc-fusion protein were cultivated in serum-free suspension cultures with 10 mM ammonium addition. The addition of ammonium to th