Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Tadas Jakočiūnas"'
Autor:
Stefano Donati, Matthias Mattanovich, Pernille Hjort, Simo Abdessamad Baallal Jacobsen, Sarah Dina Blomquist, Drude Mangaard, Nicolas Gurdo, Felix Pacheco Pastor, Jérôme Maury, Rene Hanke, Markus J. Herrgård, Tune Wulff, Tadas Jakočiūnas, Lars Keld Nielsen, Douglas McCloskey
Publikováno v:
npj Systems Biology and Applications, Vol 9, Iss 1, Pp 1-12 (2023)
Abstract Multi-omics datasets are becoming of key importance to drive discovery in fundamental research as much as generating knowledge for applied biotechnology. However, the construction of such large datasets is usually time-consuming and expensiv
Externí odkaz:
https://doaj.org/article/c89e1030bd6e4de38097125bb0d955c5
Autor:
Vasil D'Ambrosio, Lea G. Hansen, Jie Zhang, Emil D. Jensen, Dushica Arsovska, Marcos Laloux, Tadas Jakočiūnas, Pernille Hjort, Davide De Lucrezia, Serena Marletta, Jay D. Keasling, Michael K. Jensen
Publikováno v:
Synthetic and Systems Biotechnology, Vol 7, Iss 2, Pp 657-663 (2022)
The synthetic biology toolkit for baker's yeast, Saccharomyces cerevisiae, includes extensive genome engineering toolkits and parts repositories. However, with the increasing complexity of engineering tasks and versatile applications of this model eu
Externí odkaz:
https://doaj.org/article/350328b816f64150aa8b75abe0b35547
Autor:
Tadas Jakočiūnas, Andreas K. Klitgaard, Eftychia Eva Kontou, Julie Bang Nielsen, Emil Thomsen, David Romero-Suarez, Kai Blin, Christopher J. Petzold, Jennifer W. Gin, Yaojun Tong, Charlotte Held Gotfredsen, Pep Charusanti, Rasmus J.N. Frandsen, Tilmann Weber, Sang Yup Lee, Michael K. Jensen, Jay D. Keasling
Publikováno v:
Synthetic and Systems Biotechnology, Vol 5, Iss 1, Pp 11-18 (2020)
To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms is requir
Externí odkaz:
https://doaj.org/article/e0f913ee7f3644809c9f5660c13077d9
Publikováno v:
PLoS Genetics, Vol 9, Iss 10, p e1003762 (2013)
Mating-type switching in fission yeast results from gene conversions of the active mat1 locus by heterochromatic donors. mat1 is preferentially converted by mat2-P in M cells and by mat3-M in P cells. Here, we report that donor choice is governed by
Externí odkaz:
https://doaj.org/article/e509af0c255044b09a44722e83531786
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2513
Here, in this chapter, we describe a detailed protocol for the method named Cas9-mediated protein evolution reaction or short CasPER. CasPER is based on the generation of large 300-600-bp mutagenized linear DNA fragments by error-prone PCR which are
Publikováno v:
Methods in Molecular Biology ISBN: 9781071623985
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c2040d268eca95abafacfccea84fa7c7
https://doi.org/10.1007/978-1-0716-2399-2_3
https://doi.org/10.1007/978-1-0716-2399-2_3
Autor:
Julie B. Nielsen, Yaojun Tong, Emil Thomsen, Tadas Jakočiūnas, Christopher J. Petzold, Pep Charusanti, Charlotte Held Gotfredsen, Kai Blin, Jennifer W. Gin, Michael Krogh Jensen, Eftychia E. Kontou, Andreas Klitgaard, Rasmus John Normand Frandsen, Sang Yup Lee, Tilmann Weber, David Romero-Suarez, Jay D. Keasling
Publikováno v:
Synthetic and Systems Biotechnology, Vol 5, Iss 1, Pp 11-18 (2020)
Jakočiūnas, T, Klitgaard, A K, Kontou, E E, Nielsen, J B, Thomsen, E, Romero Suarez, D, Blin, K, Petzold, C J, Gin, J W, Tong, Y, Gotfredsen, C H, Charusanti, P, Frandsen, R J N, Weber, T, Lee, S Y, Jensen, M K & Keasling, J D 2020, ' Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast ', Synthetic and Systems Biotechnology, vol. 5, no. 1, pp. 11-18 . https://doi.org/10.1016/j.synbio.2020.01.004
Synthetic and Systems Biotechnology
Synthetic and systems biotechnology, vol 5, iss 1
Jakočiūnas, T, Klitgaard, A K, Kontou, E E, Nielsen, J B, Thomsen, E, Romero Suarez, D, Blin, K, Petzold, C J, Gin, J W, Tong, Y, Gotfredsen, C H, Charusanti, P, Frandsen, R J N, Weber, T, Lee, S Y, Jensen, M K & Keasling, J D 2020, ' Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast ', Synthetic and Systems Biotechnology, vol. 5, no. 1, pp. 11-18 . https://doi.org/10.1016/j.synbio.2020.01.004
Synthetic and Systems Biotechnology
Synthetic and systems biotechnology, vol 5, iss 1
To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms is requir
Autor:
Vasil, D'Ambrosio, Lea G, Hansen, Jie, Zhang, Emil D, Jensen, Dushica, Arsovska, Marcos, Laloux, Tadas, Jakočiūnas, Pernille, Hjort, Davide, De Lucrezia, Serena, Marletta, Jay D, Keasling, Michael K, Jensen
Publikováno v:
Synthetic and systems biotechnology. 7(2)
The synthetic biology toolkit for baker's yeast
Autor:
Alicia V. Lis, Lasse Ebdrup Pedersen, Michael Krogh Jensen, Tadas Jakočiūnas, Jay D. Keasling
Publikováno v:
Jakočiūnas, T, Pedersen, L E, Lis, A V, Jensen, M K & Keasling, J D 2018, ' CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9 ', Metabolic Engineering, vol. 48, pp. 288-296 . https://doi.org/10.1016/j.ymben.2018.07.001
Here we describe a method for robust directed evolution using mutagenesis of large sequence spaces in their genomic contexts. The method employs error-prone PCR and Cas9-mediated genome integration of mutant libraries of large-sized donor variants in
Autor:
Jay D. Keasling, Marcos Laloux, Michael Krogh Jensen, Tadas Jakočiūnas, Lasse Eggers Pedersen, Emil D. Jensen, Beata Joanna Lehka
Publikováno v:
Nucleic Acids Research
Nucleic acids research, vol 49, iss 15
Jensen, E D, Laloux, M, Lehka, B J, Pedersen, L E, Jakočiūnas, T, Jensen, M K & Keasling, J D 2021, ' A synthetic RNA-mediated evolution system in yeast ', Nucleic Acids Research, vol. 49, no. 15, e88 . https://doi.org/10.1093/nar/gkab472
Nucleic acids research, vol 49, iss 15
Jensen, E D, Laloux, M, Lehka, B J, Pedersen, L E, Jakočiūnas, T, Jensen, M K & Keasling, J D 2021, ' A synthetic RNA-mediated evolution system in yeast ', Nucleic Acids Research, vol. 49, no. 15, e88 . https://doi.org/10.1093/nar/gkab472
Laboratory evolution is a powerful approach to search for genetic adaptations to new or improved phenotypes, yet either relies on labour-intensive human-guided iterative rounds of mutagenesis and selection, or prolonged adaptation regimes based on na