Zobrazeno 1 - 10
of 742
pro vyhledávání: '"TRIzol"'
Publikováno v:
PeerJ, Vol 12, p e18072 (2024)
Background Extracting high-quality total RNA is pivotal for advanced RNA molecular studies, such as Next-generation sequencing and expression microarrays where RNA is hybridized. Despite the development of numerous extraction methods in recent decade
Externí odkaz:
https://doaj.org/article/bf85f80e9aeb45c8b5c69cb32d357871
Autor:
Shina Sasi, Saranya Krishnan, Preshobha Kodackattumannil, Aysha AL Shamisi, Maitha Aldarmaki, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Publikováno v:
Plant Methods, Vol 19, Iss 1, Pp 1-13 (2023)
Abstract Background High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli. But, the extraction of high-quality RNA is
Externí odkaz:
https://doaj.org/article/ce6bb19f8a864c07a8d9b4b3c5bab770
Publikováno v:
BioTechniques, Vol 74, Iss 5, Pp 203-209 (2023)
High-quality RNA isolation from recalcitrant adipose tissue with high lipid content and low cell numbers is difficult. Many studies have made efforts to optimize methods for isolating RNA from adipose tissue through combinations of column-based kits
Externí odkaz:
https://doaj.org/article/892072aa5dab42d0b69f98cc0266a89a
Publikováno v:
Journal of the Serbian Chemical Society, Vol 88, Iss 7-8, Pp 729-738 (2023)
After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to the limited efficiency of pellet reconstitution/solubilisation. As the pelleted proteins are tightly packed, they are most often solubilised u
Externí odkaz:
https://doaj.org/article/d18124187ff6448e914bcf62b6019d04
Publikováno v:
BMC Biotechnology, Vol 22, Iss 1, Pp 1-7 (2022)
Abstract Background A mixture of phenol and guanidine isothiocyanate (“P/GI”, the principal components of TRIzol™ and similar products) is routinely used to isolate RNA, DNA, and proteins from a single specimen. In time-course experiments of ce
Externí odkaz:
https://doaj.org/article/67d77552a3c3414aae70a8df130ae3a2
Publikováno v:
Plants, Vol 13, Iss 3, p 389 (2024)
Since the development of next-generation sequencing techniques and with the growing interest in transcriptomic studies, there is a demand for high-throughput RNA extraction techniques. General RNA extraction protocols are unreliable when it comes to
Externí odkaz:
https://doaj.org/article/a2d46faa6ab1492c955eeb3cdd70ed31
Akademický článek
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Akademický článek
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Autor:
Amjad Iqbal, Yaodong Yang
Publikováno v:
Methods and Protocols, Vol 6, Iss 5, p 75 (2023)
Procuring high-grade RNA from mature coconut tissues is a tricky and labor-intensive process due to the intricate scaffold of polysaccharides, polyphenols, lipids, and proteins that form firm complexes with nucleic acids. However, we have effectively
Externí odkaz:
https://doaj.org/article/5249cc2abecb4d0d9344a48606d187cd
Autor:
Judith Olejnik, Adam J. Hume, Stephen J. Ross, Whitney A. Scoon, Scott Seitz, Mitchell R. White, Ben Slutzky, Nadezhda E. Yun, Elke Mühlberger
Publikováno v:
Pathogens, Vol 12, Iss 7, p 952 (2023)
The study of highly pathogenic viruses handled under BSL-4 conditions and classified as Select Agents frequently involves the transfer of inactivated materials to lower containment levels for downstream analyses. Adhering to Select Agent and BSL-4 sa
Externí odkaz:
https://doaj.org/article/c26bcb4efaf34936bdb613d5b61dc334